| Project/Area Number |
13671549
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kansai Medical University |
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Principal Investigator |
NAKAMURA Masaya Kansai Medical University, Faculty of Medicine, Assistant Professor, 医学部, 講師 (20237431)
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| Co-Investigator(Kenkyū-buntansha) |
IIDA Hirokazu Kansai Medical University, Faculty of Medicine, Professor, 医学部, 教授 (60176047)
AKAGI Shigeo Kansai Medical University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (80148501)
MIYASHIMA Shigeo Kansai Medical University, Faculty of Medicine, Instructor, 医学部, 助手 (90288837)
ESUMI Takashi Kansai Medical University, Faculty of Medicine, Instructor, 医学部, 助手 (20351504)
IKEHARA Susumu Kansai Medical University, Faculty of Medicine, Professor, 医学部, 教授 (90108986)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | IBM-BMT / leg-transplantation / fludarabine phosphate / 下肢移植 / トレランス / Fludarabine phosphate |
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| Research Abstract |
We have recently established a new method for bone marrow transplantation(BMT) in mice : bone marrow cells(BMCs) are directly injected into the intra-bone marrow(IBM) cavity(IBM-BMT). In the present study, we carried out allogeneic leg transplantation in rats in conjunction with IBM-BMT. Brown Norway(BN: RTIA^n) rats were intravenously injected with 50mg/kg of fludarabine phosphate, followed by sublethal fractionated irradiation(4.5Gy×2) one day before IBM-BMT. The hind limb from a Fischer344(F344:RTIA^1) rat was transplanted on day 0, and BMCs(3×10^7 cells/100μ1) obtained from the donor F344 rat were injected into the bone marrow cavity of the left tibia of the recipient BN rat. The hematolymphoid cells in the recipient BN rats were completely reconstituted by the cells of the donor F344 rat. The limbs transplanted from the donor F344 rats were accepted for >1yr without any clinical signs of rejection. The lymphocytes of the BN rats showed tolerance to both donor-type and recipient-type major histocompatibility complex(MHC) determinants in mixed lymphocyte reaction, but showed a significant response to the third party MHC determinations. Using a combination of the injection of fludarabine, low-dose irradiation, and IBM-BMT, we have succeeded in allogeneic limb transplantation without using any immunosuppressants after the operation.
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