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Tissue Engineering with leg lengthening system : involvement of reactive oxygen species.

Research Project

Project/Area Number 13671551
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionKinki University

Principal Investigator

HAMANISHI Chiaki  Kinki Univ., School Med., Dept. Orthop.Surg., Professor, 医学部, 教授 (00164921)

Co-Investigator(Kenkyū-buntansha) MORI S.  Kinki Univ., School Med., Dept. Orthop.Surg., Assistant, 医学部, 助手 (80351584)
OTANI K.  Kinki Univ., School Med., Dept. Orthop.Surg., Lecturer, 医学部, 講師 (20258031)
FUKUDA K.  Kinki Univ., School Med., Dept. Orthop.Surg., Professor, 医学部, 教授 (50201744)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2003: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 2002: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 2001: ¥3,100,000 (Direct Cost: ¥3,100,000)
KeywordsChondrocyte / Mechanical stress / nitric oxide / 骨芽細胞 / フリーラジカル
Research Abstract

Many tissues or organs including bone and joint adopted their morphology under the influence of the environmental mechanical loading. The purpose of this study is to solve this mysterious process and apply it for therapeutic aspect as tissue engineering. We introduce the distraction osteogenesis, which afford to correct the deformity and lengthen the limb. Although mechanical stretch on bone, tendon and vessel is possible to apply, lag period in the bone maturation is necessary. To improve this system, we investigate the role of reactive oxygen species(ROS) under the influence of mechanical stress.
We used in vivo model of leg-lengthening *and found the characteristic morphology, such as the invasion of endothelial cells and apoptosis of mesenchymal cells. We first focused on the distribution of apoptosis of the osteoblast in this model. Recent observations revealed the significant role of ROS in ischemia-reperfusion induced cell apoptosis. We then examine the effect of mechanical stress on generation of the ROS and apoptosis of osteoblast. Cyclic tensile stretch loaded oh these cells clearly enhanced ROS synthesis in a time-and magnitude-dependent fashion. The disruption of micro filaments abolished the stress-induced ROS synthesis. Rotenone, an inhibitor of the mitochondrial electron transport chain, enhanced stress-induced ROS synthesis. These data suggest that actin filament and mitochondria are involved in this action.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] N.Yamamoto, C.Hamanishi, et al.: "Cyclic tensile stretch stimulates the release of reactive oxygen species from osteoblast-like cells"Calcif Tissue Int.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Matsukawa, C.Hamanishi, et al.: "Enhancement of nitric oxide and proteoglycan synthesis due to cyclic tensile strain loaded on chondrocytes attached to fibronecti"Inflamm Res. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Matsushita, C.Hamanishi, et al.: "Effect of ebselen, a scavenger of reactive oxygen species, on chondrocyte metabolism"Modern Rheum. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamazaki K, C.Hamanishi, et al.: "Cyclic tensile stretch loaded on bovine chondrocytes causes depolymerization of hyaluronan"Arthritis Rheum.. 48・11. 3151-3158 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamazaki K, Hamanishi C., et al.: "Reactive oxygen species depolymerize hyaluronan : involvement of the hydroxyl radical"Pathophysiology. 9・47. 215-220 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kikuyama A, Fukuda K, et al.: "Hydrogen peroxide induces apoptosis of osteocytes : involvement of calcium ion and caspase activity"Calcif Tissue Int. 71・3. 243-248 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] N.Yamamoto, K.Fukuda, T.Matsushita, M.Matsukawa, F.Hara, C.Hamanishi: "Cyclic tensile stretch stimulates the release of reactive oxygen species from osteoblast-like cells."Calcif Tissue Int. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] M.Matsukawa, K.Fukuda, K.Yamasaki, K.Yoshida, H.Munakata, C.Hamanishi: "Enhancement of nitric oxide and proteoglycan synthesis due to cyclic tensile strain loaded on chondrocytes attached to fibronectin"Inflamm Res. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] T.Matsushita, K.Fukuda, H.Yamamoto, K.Yamazaki, T.Tomiyama, M.Oh, C.Hamanishi: "Effect of ebselen, a scavenger of reactive oxygen species, on chondrocyte metabolism"Modern Rheum. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Yamazaki, K.Fukuda, M.Matsukawa, F.Hara, T.Matsushita, N.Yamamoto, K.Yoshida, H.Munakata, C.Hamanishi: "Cyclic tensile stretch loaded on bovine chondrocytes causes depolymerization of hyaluronan : involvement of reactive oxygen species."Arthritis Rheum.. 48(11). 3151-3158 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Yamazaki, K.Fukuda, M.Matsukawa, F.Hara, K.Yoshida, M.Akagi, H.Munakata, C.Hamanishi: "Reactive oxygen species depolymerize hyaluronan : involvement of the hydroxyl radical."Pathophysiology.. 9(4). 215-220 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] A.Kikuyama, K.Fukuda, S.Mori, M.Okada, H.Yamaguchi, C.Hainanishi: "Hydrogen peroxide induces apoptosis of osteocytes : involvement of calcium ion and caspase activity."Calcif Tissue Int. 71(3). 243-248 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] N Yamamoto, C Hamanishi, et al.: "Cyclic tensile stretch stimulates the release of reactive oxygen species from osteoblast-like cells"Calcif Tissue Int.. in press.

    • Related Report
      2003 Annual Research Report
  • [Publications] M Matsukawa, C Hamanishi, et al.: "Enhancement of nitric oxide and proteoglycan synthesis due to cyclic tensile strain loaded on chondrocytes attached to fibronectin"Inflamm Res. in press.

    • Related Report
      2003 Annual Research Report
  • [Publications] T Matsushita, C Hamanishi, et al.: "Effect of ebselen, a scavenger of reactive oxygen species, on chondrocyte metabolism"Modern Rheum. in press.

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamazaki K, C.Hamanishi, et al.: "Cyclic tensile stretch loaded on bovine chondrocytes causes depolymerization of hyaluronan"Arthritis Rheum. 48・11. 3151-3158 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamazaki K, Hamanishi C, et al.: "Reactive oxygen species depolymerize hyaluronan : involvement of the hydroxyl radical"Pathophysiology. 9・47. 215-220 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kikuyama A, Fukuda K, et al.: "Hydrogen peroxide induces apoptosis of osteocytes : involvement of calcium ion and caspase activity."Calcif Tissue Int. 71・3. 243-248 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kikuyama A, Fukuda K, et al.: "Hydrogen peroxide induces apoptosis of osteocytes : involvement of calcium"Calcif Tissue Int.. 71・3. 243-248 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sohen S, Fukuda K et al.: "Activation of histamine H1 receptor results in enhanced proteoglycan synthesis by human articular chondrocyte : involvement of protein kinase C and intracellular Ca(2+)"Pathophysiology. 8・2. 93-98 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fukuda K. et al.: "Sodium hyaluronate inhibits interleukin-1-evoked"Osteoarthritis Cartilage. 9・4. 390-392 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nishisaka F, Fukuda K, et al.: "Interleukin-4 reversed the Interleukin 1-inhibited proteoglycan synthesis"Pathophysiology. 7・4. 289-293 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hara F.Fukuda K.et al.: "Cyclic tensile stretch inhibition of nitric oxide release from osteoblast-like cells is both G protein and actin-dependent"J Orthop Res.. 19.1. 126-131 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Asada S, Fukuda K, et al.: "Hydrogen peroxide induces apoptosis of chondrocytes ; involvement of calcium ion and extracellular signal-regulated protein kinase"Inflamm Res. 50・1. 19-23 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sohen S, Hamanishi C, et al.: "Activation of histamine H1 receptor results in enhanced proteoglycan synthesis by human articular chondrocyte"Pathophysiology. 8(2). 93-98 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Fukuda K, Hamanishi C, et al.: "Sodium hyaluronate inhibits interleukin-1-evoked reactive oxygen species of bovine articular chondrocytes"Osteoarthritis Cartilage. 9(4). 390-392 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hara F, Hamanishi C, et al.: "Cyclic tensile stretch inhibition of nitric oxide release from osteoblast-like cells is both G protein and actin-dependent"J Orthop Res. 19(1). 126-131 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Asada S, Hamanishi C, et al.: "Hydrogen peroxide induces apoptosis of chondrocytes ; involvement of calcium ion and extracellular signal-regulated protein kinase"Inflamm Res. 50(1). 19-23 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nishisaka F, Hamanishi C, et al.: "Interleukin-4 reversed the Interleukin-1-inhibited proteoglycan synthesis through the inhibition of NO release"Pathophysiology. 7(4). 289-293 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nonaka T, Hamanishi C, et al.: "Hyaluronic acid inhibits the expression or u-PA, PAT-T, and u-PAR in human synovial fibroblasts of osteoarthritis and rheumatoid arthritis"J Rheum. 27(4). 997-1004 (2000)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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