| Project/Area Number |
13671578
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Osaka University |
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Principal Investigator |
FUJINO Yuji OSAKA UNIVERSITY HOSPITAL, ASSISTANT PROFESSOR, 医学部附属病院, 助手 (50252672)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIMURA Masaji OSAKA UNIVERSITY HOSPITAL, LECTURER, 医学部附属病院, 講師 (10172701)
UCHIYAMA Akinori OSAKA UNIVERSITY HOSPITAL, ASSISTANT PROFESSOR, 医学部附属病院, 助手 (00324856)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | colored-microsphere / microbubble / liver blood flow / diaphragm blood flow / mechanical ventilation / pressure support ventilation / pressure controlled ventilation / 肝組織血流 / マイクロバブル法 / マイクロスフェア法 / 臓器血量 / 蛍光マイクロスフェア法 |
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| Research Abstract |
At first organ blood flow measurements were conducted in a rabbit model using the colored-microsphere method to establish the liver tissue blood flow measurement by the micobubble method. Although mechanical ventilation reduces the respiratory muscle workload, the effects of ventilatory settings on blood flows of respiratory muscle and major organs have been little studied. Using the different-colored microspheres method, we measured the regional blood flow of respiratory muscle and major organs at different mechanical ventilatory settings in a rabbit model. Ten New Zealand white rabbits were anesthetized by continuous infusion of ketamine and propofol and then tracheostomized. A 19G catheter was inserted into the left ventricle via the left carotid artery to inject colored microspheres. The right femoral artery was cannulated for blood sampling. The regional blood flows in diaphragm (Diaphcost, crur), kidney and liver were studied during pressure support ventilation (PSV) of 0, 6, 12 cmH_2O and then pressure controlled ventilation (PCV) of 6 and 12 cmH_2O after the administration of muscle relaxant. Blood flows in diaphragm during PSV6, PCV 6, 12cmH_2O were significantly smaller than that during PSV0cmH_2O (p<0.05). Blood flows in other organs including kidney and liver did not change significantly among ventilatory settings. We speculate that the difference of blood flow in diaphragm was derived from the inspiratory asynchrony between the ventilator and the animal. With this study we could establish a method to measure organ blood flow. Now we are trying to establish the microbubble method to measure regional liver tissue blood flow using the values measured by the coloredmicrosphere method as control values.
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