| Project/Area Number |
13671588
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Anesthesiology/Resuscitation studies
|
|---|
| Research Institution | Kagawa Medical University |
|---|
Principal Investigator |
OGURA shinji Kagawa Medical University Hospital, Associate professor, 医学部附属病院, 助教授 (30185566)
|
|---|
| Project Period (FY) |
2001 – 2002
|
| Project Status |
Completed (Fiscal Year 2002)
|
| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
|
|---|
| Keywords | Gram-positive bacteria / shock / sepsis / anti-thrombin III / super-antigen / cytokine / AT3 |
|---|
| Research Abstract |
Background: Superantigens are suspected to be the potent and lethal pathogens of gram-positive sepsis, and a new therapy that targeted to superantigens are required. Methods: A mixed infection model was developed in rabbits by the cecal ligation and puncture and the intraperitoneal injection of Staphylococcus aureus, which produces toxic shock syndrome toxin 1 (TSST-1). Animals were also hemoperfused with a superantigen-adsorbing device (SAAD), or a control column. Results: The model animals revealed multiple organ failure and died 6-12 hr after the injection of S. aureus. The plasma levels of TSST-1, but not of lipopolysaccharide (LPS), correlated well with mean arterial pressure (r=-0.63). Plasma TSST-1 level was significantly reduced and shock-onset time was significantly retarded in the SAAD group, although the survival time was not significantly affected. Conclusions: The animal model developed could serve as a model for sepsis. It is suggested that there is the potential application of SAAD in treating superantigen-related sepsis.
|
