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Immunopathological aspects of the interruption of germ cell transport and on testicular blood flow in mice.

Research Project

Project/Area Number 13671655
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionTokyo Medical University

Principal Investigator

ITOH Masahiro  Tokyo Med. Univ., Medicine, Proffessor, 医学部, 教授 (00232471)

Co-Investigator(Kenkyū-buntansha) SUNA Shigeru  Kagawa University, Faculty of Medicine, Dept.public health, Research Assistant, 医学部, 助手 (40253265)
NAKAMURA Yoichi  Tokyo Med.Univ., Medicine, Senior Lecturer, 医学部, 講師 (40147210)
TERAYAMA Hayato  Tokyo Med.Univ., Medicine, Research Assistant, 医学部, 助手 (00384983)
NAITO Munekazu  Tokyo Med.Univ., Medicine, Research Assistant, 医学部, 助手 (10384984)
飯村 彰  神奈川歯科大学, 歯学部, 助手 (30211022)
林 省吾  東京医科大学, 医学部, 助手 (60349496)
竹内 義喜  香川大学, 医学部, 教授 (20116619)
市川 早苗  東京医科大学, 医学部, 助教授 (60112786)
Project Period (FY) 2001 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordstestis / ischemia / inflammation / germ cell transport / spermatogenesis
Research Abstract

1. Primary functions of the testis are spermatogenesis and testosterone secretion.
2. The spermatozoa are very special in that they appear most late in human body. Therefore, their autoantigens are apt to be recognized as foreign bodies because of the appearance of them long after the period of immune tolerance.
3. It has been considered that the spermatozoa are preserved from the immune system by the blood-testis barrier formed by inter-Sertoli cell junctions. However, our studies revealed that the blood-testis barrier is opened to the immune system at the tubuli recti and rete testis regions. Therefore, the sperm antigens are accessible to the immune cells under normal condition.
4. Treatment with anti-cytokines (IL-6 and IL-10) antibodies and a immunomodulater (deoxyspergualin) augmented or inhibited the testicular autoimmunity.
5. Experimental autoimmune orchitis has been most studied in a field of investigation of the testicular autoimmunity. In the present study, we tried to evaluate autoimmune aspects of testes in which their germ cell transport or blood flow is experimentally interrupted in mice.
6. In another study, we found that traumatic injury to a testis on one side induced lymphocytic infiltration and aspermatogeneis in testis on opposite side. However, in experiments of interruption of germ cells transport or blood flow in a testis on one side, no inflammatory or degenerative changes were found in a testis on opposite side. In further studies, we combine these experiments with sensitization with sperm autoantigens or pretreatment with endocrine disruptors to investigate whether the additional treatments modulate immune state of the testes.

Report

(5 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (15 results)

All 2005 2003 2002 2001 Other

All Journal Article (11 results) Publications (4 results)

  • [Journal Article] Tissue microcircumstances for leukocytic infiltration into the testis and epididymis in mice2005

    • Author(s)
      Itoh M, Terayama H, Naito M, Ogawa Y, Tainosho S
    • Journal Title

      Journal of Reproductive Immunology (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] 精巣機能とサイトカイン2003

    • Author(s)
      伊藤正裕
    • Journal Title

      Hormone Frontier in Gynecology 10

      Pages: 51-57

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Proinflammatory effects of exogenously administered IL-10 in experimental autoimmune orchitis2003

    • Author(s)
      Kaneko T, Itoh M, Nakamura Y, Iimura A, Hayashi S, Takahashi K, Stivala F, Bendtzen K, Nicoletti F
    • Journal Title

      Cytokine 22

      Pages: 50-53

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Testicular functions and its cytokines2003

    • Author(s)
      Itoh M
    • Journal Title

      Hormone Frontier in Gynecology Vol.10

      Pages: 51-57

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Proinflammatory effects of exogenously administered IL-10 in experimental autoimmune orchitis2003

    • Author(s)
      Kaneko T, Itoh M, Nakamura Y, Iimura A, Hayashi S, Takahashi K, Stivala F, Bendtzen K, Nicoletti F
    • Journal Title

      Cytokine vol.22

      Pages: 50-53

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Prevention of murine experimental autoimmune orchitis by recombinant human interleukin-62002

    • Author(s)
      Li L, Itoh M, Ablake M, Macri B, Bebdtzen K, Nicoletti F
    • Journal Title

      Clinical Immunology 102(2)

      Pages: 135-137

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Short-term prophylaxis with deoxyspergualin prevents testicular autoimmunity in mice2002

    • Author(s)
      Ablake M, Itoh M, Kaneko T, Iimura A, Nakamura Y, Meroni P, Nicoletti F
    • Journal Title

      European Journal of Pharmacology 450

      Pages: 209-212

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Prevention of murine experimental autoimmune orchitis by recombinant human interleukin-62002

    • Author(s)
      Li L, Itoh M, Ablake M, Macri B, Bendtzen K, Nicoletti F
    • Journal Title

      Clinical Immunology Vol.102 (2)

      Pages: 135-137

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Prevention of murine experimental autoimmune orchitis by recombinant human interleukin-62002

    • Author(s)
      Li L, Itoh M, Ablake M, Macri B, Bendtzen K, Nicoletti F
    • Journal Title

      Clinical Immunology 102(2)

      Pages: 135-137

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 生殖免疫-場の観察2001

    • Author(s)
      伊藤正裕
    • Journal Title

      東京医科大学雑誌 59(6)

      Pages: 439-446

    • NAID

      10010012437

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Reproductive Immunology2001

    • Author(s)
      Itoh M
    • Journal Title

      The Journal of Tokyo Medical University 59(6)

      Pages: 439-446

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Kaneko T, Itoh M, Nakamura Y, Iimura A, Hayashi S 他4名: "Proinflammatory effects of exogenously administered IL-10 in experimental autoimmune orchitis."Cytokine. Vol.22. 50-53 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ablake M, Itoh M, Terayama H, Hayashi S, Shoji S 他3名: "Di-(2-ethylhexyl) phthalate induces severe aspermatogenesis in mice, however, subsequent antioxidant vitamins supplementation accelerates regeneration of the seminiferous epithelium."International Journal of Andrology. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Li L, Itoh M, Ablake M 他3名: "Prevention of murine experimental autoimmune orchitis by recombinant human interleukin-6"Clinical Immunology. Vol.1 102(2). 135-137 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ablake M, Itoh M, Kaneko T, Iimura A, Nakamura Y 他2名: "Short term prophylaxis with deoxyspergualin prevents testicular autoimmunity in mice"European Journal of Phamacology. (in press). (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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