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A proof of angiogenesis in bladder cancer using reconstructed bladder mucosa

Research Project

Project/Area Number 13671657
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research Institution佐賀医科大学

Principal Investigator

NAKASHIMA Keiji (2003)  Saga University, Faculty of Medicine, Urology, Assistant Professor, 医学部, 助手 (00346884)

藤山 千里 (2001-2002)  佐賀医科大学, 医学部, 講師 (80209113)

Co-Investigator(Kenkyū-buntansha) NANRI Masaharu  Saga University, Faculty of Medicine, Urology, Assistant Professor, 医学部, 助手 (80346890)
MASAKI Zenjiro  Saga University, Faculty of Medicine, Urology, Professor, 医学部, 教授 (40038716)
中島 啓二  佐賀医科大学, 医学部, 助手 (00346884)
塚原 常宏  佐賀医科大学, 医学部, 助手 (70315208)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordscell culture / bladder cancer / urothelial cell / angiogenesis / collagen / vascular / endothelium fibroblast / VEGF
Research Abstract

Using reconstruction of the urinary bladder mucosa in three-dimensional collagen gel culture, we investigated the role of angiogenic factor in bladder cancer by a series of histological observation.
Normal transitional epithelial cells isolated from porcine bladder were cultured on reconstructed lamia propia using fibroblasts.in type I collagen gel. The inoculated transitional epithelial cells grew, in a stratified fashion and closely resembled urothelium in viva Human urinary bladder carcinoma cell (HUBCC) line (HT-1197, RT-4, T24) was seeded onto this model. HT 1197 cells were infiltrated in lamia propia. Next, vascular endothelium and epidermoid growth factor (EGF) were added into the, collagen gel and cultured. But the proliferations of HUBCC were equal to the fibroblasts alone model.
Then in order to evaluate the angiogenesis in vivo, we transplanted the reconstructed transitional epithelium HUBCC was seeded to rats and tried to observe the angiogenesis in histologically. However all rats died few days later from transplantation and the grafts did not adhere in autopsy. In vitro," HUBCC was seeded onto the reconstructed mucosa again and sections were analyzed for expression of vascular endothelial growth factor (VEGF) protein. However no VEGF protein expression was observed. And expression of hypoxia-inducible factor l a and thymidine phospholylase were not observed in Western blotting.
We were not able to prove the angiogenesis in ladder cancer in this study, so we have to develop new technique.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] K.Nakamura: "Bladder cancer cell implantation in reconstructed bladder in vitro : a model of tumor recurrence"British Journal of Urology International. 89. 119-125 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 塚原常宏: "In vitro 人工尿路上皮作製の試み"日本小児泌尿器科学会雑誌. 11・1. 63 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Zhenxian du: "Hif-la protein expression in human normal, benign and malignant prostate"日本泌尿器科学会雑誌. 94・2. 383 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 塚原常宏: "In vitro 人工尿路上皮再構築の試み"日本泌尿器科学会雑誌. 94・2. 367 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Du Zhenxian: "Expression of hypoxia inducible factor 1α in human normal, benign and malignant prostate tissue"Chinese Medical Journal. 116・12. 1936-1939 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 藤山千里: "人工尿路上皮再構築の試み"日本泌尿器科学会雑誌. 95・2. 148 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] K.Nakamura: "Bladder cancer cell implantation or reconstructed bladder in vitro : a model of tumor recurrence"British Journal of Urology International. 89. 119-125 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Du Zhenxian: "Expression of hypoxia, inducible factor 1α in human normal, benign and malignant prostate tissue"Chinese Medical Journal. 116(12). 1936-1939 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 藤山千里: "人工尿路上皮再構築の試み"日本泌尿器科学会雑誌. 95(2). 148 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] K.Nakamura, C.Fujiyama, Y.Tokuda, H.Sugihara, Z.Masaki: "Bladder cancer cell implantation in reconstructed bladder in vitro : a model of tumour recurrence"British Journal of Urology International. 89. 119-125 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 塚原常宏, 藤山千里, 真崎善二郎: "In vitro人工尿路上皮作成の試み"日本小児泌尿器科学会雑誌. 11・1. 63 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Zhenxian du: "Hif-la protein expression in human normal, benign and malignant prostate"日本泌尿器科学会雑誌. 94・2. 383 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 塚原常宏, 藤山千里, 真崎善二郎: "In vitro人工尿路上皮再構築の試み"日本泌尿器科学会雑誌. 94・2. 367 (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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