| Project/Area Number |
13671660
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
ITOH Naoki Sapporo Medical University, School of Medicine, Associate Professor, 医学部, 助教授 (60193504)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Satoshi Sapporo Medical University, School of Medicine, Senior Instructor, 医学部, 助手 (30332919)
MASUMORI Naoya Sapporo Medical University, School of Medicine, Assistant Professor, 医学部, 講師 (20295356)
TSUKAMOTO Taiji Sapporo Medical University, School of Medicine, Professor, 医学部, 教授 (50112454)
HOTTA Hiroshi Sapporo Medical University, School of Medicine, Senior Instructor, 医学部, 助手 (90336404)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | prostate / inflammatory cell / chemokine / IL-8 / Gatifloxacin(GFLX) |
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| Research Abstract |
(1)The pathological study of inflammatory cell infiltration in the prostate in patients
Patients entered into the study received radical cystoprostatectomy because of invasive bladder cancer in our institute from 1998 to 2000. The infiltrating cell type was assessed by immunostaining using a series of antibodies CD2O, CD45RO, CD68,S-100. The types of infiltrating cells were dominantly T cells. Inflammatory cell infiltration was seen in 32.4% cases.
(2)Antimicrobial agents and suppression of cytokine secretion of prostate cancer cell line.
PC-3,a human prostate cancer cell lines, was used for the experiment. IL-8 concentrations of the supematants were increased depending on the concentrations of Mycoplasma hominis. The mRNA of Toll like receptor-2 and Toll like receptor4 were demonstrated by RT-PCR in PC-3 cell lines. The activities of NF-κB were increased depending on the concentration of Mycoplasma hominisa. These data show that the signals from Toll-like receptor up the activities of NF-κB and lead to produce of IL-8. The influence of GFLX on IL-8 mRNA expression was analyzed using RT-PCR. GFLX significantly attenuated the TNF-α induced IL-8 mRNA level. GFLX suppresses the secretion of IL-8 from PC-3 in a dose-dependent manner. The results may imply that GFLX has an anti-inflammatory effect on chronic prostatitis.
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