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Human follicular development flollowing xenotransplantation of human pnarian cortex into immune deficient mice

Research Project

Project/Area Number 13671688
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionTohoku University

Principal Investigator

TERADA Yukihiro  Tohoku University, Hospital, Associate Professor, 医学部附属病院, 助教授 (10260431)

Co-Investigator(Kenkyū-buntansha) MURAKAMI Takashi  Tohoku University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (20240666)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2001: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsOvarian transplatation / Human follicle / Immune dificient mouse / Steroidgenic enzymes / human ovary / transplantation / NOD SCID mouse / steroidgenesis / ovarian tumor / 卵巣凍結保存 / 卵胞発育
Research Abstract

We studied the immunohistochemical expression and localization of steroidogenic enzymes in the graft of non-pathological human ovary following xenogenic transplantation into a non-obese diabetic severe combined immune deficient (NOD-SCID) mice. Human follicles following xenotransplantation into NOD-SCID mice were studied by immunohistochemistry antibodies against steroidgenic enzymes. In the pre antral follicles of these grafts, steroidogenic enzymes, p450 scc,p450 c17 and 3β-HSD were present in only the the theca cell layer, observations which are consistent with the findings of non-pathological human ovarian cortex. Cytochrome p450 aromatase and estrogen receptor, however, were not detected in these grafts, and these follicles did not possess specific feature of a dominant follicle. These findings suggest that the expression of steroidogenic enzymes in humnan follicles following xenogenic transplantation into NOD-SCID mice. is similar to that of non-pathological human ovaries. Howeve … More r, these follicles do not possess features of dominant follicles. which are known to develop into the corpus luteum. Furthermore, we succeed the successful generation of mature human graafian follicles following xenotransplantation of a human ovarian cortex into a NOD/SCID/g cnull mouse. Ten weeks after transplantation, we stimulated the ovary tissue through daily intraperitoneal injections of human menopausal gonadotropin for 14 days. Ovarian grafts were removed from recipient mice twelve weeks after transplantation. In 2 out of 8 transplanted mice. human graafian follicles l6-18 mm in diameter were obtained from transplanted tissues within the ovarian bursa. High levels of cytochrome p450 aromatase and estroqen receptor expression were observed in both the granulosa and the human graafian follicle theca layer developed in tne murine ovarian bursa. These results suggest that these follicles have similar characteristics as the dominant follicles of a normal premenopausal cycling human ovary. Less

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Sato Y, Terada Y, et al..: "Immunohistochemical localization of steroidogenic enzymes in human follicle following xenotransplantation of the human ovarian cortex into NOD-SCID mice"Molecular Reproduction and Development. 65. 67-72 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Terada Y. et al.: "Intracytoplasmie sperm injection (ICSI) : Stilleto Conception or a Stab in the Dark. (Review)"Archieves of Audiology. 49. 169-177 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakamuta S, Terada Y.et al.: "Anakysis of the human sperm centrosomal function and the oocyte activation in a case of globozoospermia by ICSI into bovine oocytes"Human Reproduction. 17. 2930-2934 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Rawe V, Terada Y, et al.: "A pathology of the sperm centriole responsible for defective sperm aster formation, sygamy and cleavage"Human Reproduction. 17. 2344-2349 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Terada Y, et al.: "Human sperm aster formation after intracytoplasmic sperm injection with rabbit and bovine eggs"Fertil Sieril. 77. 1283-1284 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sato Y., Terada Y., Utsunomiya H., Koyanagi Y, Okamura K.: "Immunohistochemical localization of steroidogenic enzymes in human follicle following xenotransplantation of the human ovarian cortex into NOD-SCID mice."Molecular Reproduction and Development. 65. 67-72 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Terada Y., Nakamura S., Morita J., Hewitson L., Sinerly CR., Murakami T., Yaegashi N., Schatten G., Okamura K.: "lntracytoplasmic sperm injection (ICSI) : Stilleto Conception or a Stab in ths Dark."Archieves ofl Andrology (Review). 49. 169-177 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakamura S., Tearada Y., Horiuchi T., Emuta C., Murakami T., Yaegashi N., Okamura K.: "Anakysis of the human sperm centrosomal function and the oocyte activation in a case of globozoospermia by ICSI into bovine oocytes."Human Reproduction. 17. 2930-2934 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Rawe V., Terada T., Nakamura S., Chillik CF., Brugo Olmedo S., Chemes HE.: "A pathology of the sperm centriole responsible for defective sperm aster formation, sygarmy and cleavage."Human Reproduction. 17. 2344-2349 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Terada Y., Nakamura S., Hewitson L., Sinerly CR., Horiuchi T., Murakami T., Okamura K., Schatten G.: "Human sperm aster formation after intracytoplasmic sperm injection with rabbit and bovine eggs."Fertil Steril. 77. 1283-1284 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sato Y.Terada Y.et al.: "Immunohistochemical localization of steroidogenic enzymes in human follicle following xenotransplantation of the human ovarian cortex into NOD-SCID mice"Molecular Reproduction and Development. 65. 67-72 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Terada Y, et al.: "Intracytoplasmic sperm injection (ICSI) : Stilleto Conception or a Stab in the Dark? (Review)"Archieves of Andrology. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakamura S.Terada Y, et al.: "Anakysis of the human sperm centrosomal function and the oocyte activation in a case of globozoospermia by ICSI into bovine oocytes"Human Reproduction. 17. 2930-2934 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Rawe V, Terada Y, et al.: "A pathology of the sperm centriole responsible for defective sperm aster formation, sygamy and cleavage"Human Reproduction. 17. 2344-2349 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Terada Y, et al.: "Human sperm aster formation after intracytoplasmic sperm injection with rabbit and bovine eggs"Fertility and Sterility. 77. 1283-1284 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 寺田幸弘 他: "卵巣移植の免疫免疫抑制マウスへのヒト卵巣皮質の移植系について"産婦人科治療. 84巻. 1093-1096 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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