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Establishment of telomerase-based novel gene therapy and diagnosis for gynecologic tumors

Research Project

Project/Area Number 13671702
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionKanazawa University

Principal Investigator

KYO Satoru  Kanazawa University Obstetrics and Gynecology Asist. Prof., 大学院・医学系研究科, 講師 (50272969)

Co-Investigator(Kenkyū-buntansha) TANAKA Masaaki  Kanazawa University Obstetrics and Gynecology Asist. Prof., 医学部附属病院, 助手 (70283140)
INOUE Masaki  Kanazawa University Obstetrics and Gynecology Prof., 大学院・医学系研究科, 教授 (10127186)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsTelomerase / Gene therapy / Diagnosis / hTR / hTERT / Gynecologic tumors / 遺伝子診断hTERT
Research Abstract

To examine the possibility of novel tumor markers, telomerase activity was measured by the TRAP assay using blood samples from patients with various gynecologic tumors. To purify the epithelial components in blood samples, beads-based method was used, in which blood cells were incubated with beads that were conjugated with specific anti-epithelial antigen. Approximately, 20% of patients exhibited telomerase activity in this method, all of them had advanced gynecologic tumors, such as cervical and ovarian cancers, while none of 40 normal healthy volunteers showed no telomerase activity. Interestingly, telomerase activity observed in these patients decreased after initial treatment, such as chemotherapy and radiotherapy, suggesting that it might reflect disease progression.
We also examined the possibility of telomerase-based screening method for endometrial cancers using exfoliated endometrial scarping samples. We could achieved more than 80% of sensitivity to detect endometrial cancers using TRAP assay with endometrial scarping samples. Combination of this method with classical cytologic smear test may improve sensitivity and specificity to screen endometrial cancers.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Kyo S, Masutomi K, Maida M, et al.: "Significance of immunological detection of hTERT : re-evaluation of expression and localization of hTERT"Am. J. Pathol.. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tanaka M, Kyo S, Kanaya T, et al.: "Evidence of monoclonal composition of human endometrial epithelial glands and mosaic pattern of clonal distribution in luminal epithelium"Am. J. Pathol.. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kyo S, Inoue M.: "Complex regulatory mechanisms of telomerase activity in normal and cancer cells : How can we apply them for cancer therapy?"Oncogene. 21. 688-697 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Maida Y, Kyo S, Kanaya T et al.: "Is the telomerase assay useful for screening of endometrial lesions"Int. J. Cancer. 100. 714-718 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yatabe N, Kyo S, Kondo S. et al.: "2-5A antisense therapy directed against human telomerase RNA inhibits telomerase activity and induces apoptosis without telomere impairment in cervical cancer cells"Cancer Gene Ther.. 9. 624-630 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Maida M, Kyo S, Kanaya T. et al.: "Direct activation of telomerase by EGF through Etsーmediated transa ctivation of TERT via MAP kinase signaling pathway"Oncogene. 21. 4071-4079 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kyo S, Masutomi K, Maida M, et al.: "Significance of immunological detection of hTERT : re-e valuation of expression and localization of hTERT"Am. J. Pathol.. in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tanaka M, Kyo S, Kanaya T.et al.: "Evidence of monoclonal composition of human endom etrial epithelial glands and mosaic pattern of clonal di stribution in luminal epithelium"Am. J. Pathol.. in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kyo S and Inoue M: "Complex regulatory mechanisms of telomerase activity in normal and cancer cells : How can we apply the m for cancer therapy?"Oncogens. 21. 688-697 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Maida Y, Kyo S, Kanaya T et al.: "Is the telomerase assay useful for screening of endo metrial lesions"Imt. T. Cancer. 100. 714-718 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yatabe N, Kyo S, Kondo S. et at.: "2-5A antisense therapy directed against human telomerase RNA inhibits telomerase activity and induces apoptosis without telomere impairment in cervical cancer cells."Cancer Gene Ther.. 9. 624-630 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Maida M, Kyo S, Kanaya T. et al.: "Direct activation of telomerase by EGF through Ets - mediated transactivation of TERT via MAP kinase sign aling pathway"Oncogene. 21. 4071-4079 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kyo S, Jnoue M: "Complex regulatory mechanisms of telomerase activity in normal and cancer cell : How can we apply them for cancer therapy?"Oncogene. 21. 688-697 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kyo S, Inone M: "How to inhibit telomerase activity for cancer therapy"Curr. Med. Chem Anti-Cancer Agents. 2. 613-626 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Komata T, Kyo S et al.: "Caspace-8 gene therapy using human telomerase reverse transcrptase promoter for malignant glioma cells"Human Gene Ther. 13. 1015-1025 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Wang Z, Kyo S et al.: "Tamoxifen regulates human telomerase reverse transcriptase (hTERT) gene expression differently in breast and endometrial cancer cells"Oncogen. 21. 3517-3524 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Maida M, Kyo S et al.: "Direct activation of telomerase by EGF through Ets-mediated transactivation of TERT via MAP kinase signaling pathway"Oncogene. 21. 4071-4079 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yatabe N, Kyo S et al.: "2-5A antisense therapy directed against human telomerase RNA inhibits telomerase activity and induces apoptosis without telomere impairment in cervical cancer cells"Cancer Gene Ther. 9. 624-630 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Maida Y, Kyo S et al.: "Is the telomerase assay useful for screening of endometrial lesions?"Int. J. Cancer. 100. 714-718 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kyo S, et al.: "Complex feyrdatory mechanisms of tehmernse athority in normal and cancer cells"Onwglne. 21. 688-697 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Maida Y., Kyo S., et al.: "Direct activation of telomerse by EGF through Ets-medintic transnitirtion of TERT ria MAP Kinase Signaling PwAh wny"Onwglne. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Takakura M., Kyo S., et al.: "elomerase activation by histone deacetylase inhibitor in normal cells"Nucleic Acids Reserch. 29. 3006-3011 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Oh ST. Kyo S, et al.: "Telomerase Activation by Human Papillomavirus Type 16 E6 Protein: Induction of Human Telomerase Reverse Transcriptase Expression through Myc and GC-Rich Sp1 Binding Sites"Jounal of Virology. 75. 5559-5566 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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