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Molecular bases of the invasion of extavillous trophoblast to the decidua in human placenta

Research Project

Project/Area Number 13671714
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research Institutionkobe University

Principal Investigator

MATSUO Hiroya  Kobe University School of Medicine, Professor, 医学部, 教授 (60229432)

Co-Investigator(Kenkyū-buntansha) MARUO Takeshi  Kobe University School of Medicine, Professor, 医学部, 教授 (60135811)
中後 聡  神戸大学, 医学部, 助手 (50283891)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsplacenta / EVT / thyroid hormone / apoptosis / CAM / matrigel / invasion / decidua / Bcl-2蛋白 / Fas / 絨毛外トロホブラスト / トロホブラスト / 妊娠中毒症 / 増殖能
Research Abstract

First, we have demonstrated the expression of apoptosis-regulating proteins, Fas, Fas-L and Bcl-2 protein, and apoptosis in EVT of term placental bed. Apoptosis was more abundant in the invasive phenotype of EVT than in the proliferative phenotype of EVT and this may be indispensable for the exquisite regulation of EVT invasion to the decidua. The increased occurrence of apoptosis in the invasive phenotype of EVT was consistent with the abundant expression of Fas and Fas-L and less expression of Bcl-2 protein in those cells. It seems likely that apoptosis in EVT along the invasion to the decidua may be regulated by the interaction of these determinants.
Furthermore, we investigated on the effect of T_3 on the invasive potential of EVT through Matrigel transwells and CAMs (matrix metalloproteinase (MMP) -2, -3, tissue inhibitor metalloproteinase (TIMP) -1, fetal fibronectin (FFN) and integrin α5 β1) expression with using cultured early placental EVT.
An optimal concentration of T_3 (10^<-8>mol/L) increased the number of invading cells and the expressions of cell adhesion molecules in cultured early placental EVT. The optimal concentration of T_3 (10^<-8>mol/L) may be a key factor up-regulating the invasive potential of EVT to the decidua.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Matsuo H: "Prediction of the change in bone mineral density induced by gonadotropin-releasing hormone agonist treatment for endometriosis"Fertility Sterility. 81. 149-153 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takekida S, Matsuo H, T, Maruo T: "GnRH agonist action on granulosa cells at varying follicular stages"Mol Cell Endocrinol. 202. 155-164 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamanaka Y, Matsuo H, Nakago S, Maruo T: "Effects of estriol on cell viability and 1,25-Dihydroxyvitamin D3 receptor mRNA expression in cultured human osteoblast-like cells(HOS)."Gyynecol Endocrinl. 17. 455-461 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Murakoshi H, Matsuo H, Maruo T: "Expression of Fas/Fas-ligand, Bcl-2 protein and Apoptosis in Extravillous Trophoblast along Invasion to the Decidua in Human Term Placenta."Endocrine Journal. 50. 199-207 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shirosita Y, Matuo H: "The relationship between clinical characteritics and pregnancy outcome in preeclanpsia"J Perinat Med. 31. 194-194 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 松尾博哉, 丸尾 猛: "子宮筋腫の発育に関わる分子内分泌機構.In : Annual Review内分泌・代謝2004(金澤康徳, 武谷雄二編)"中外医学社. 5 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Marukoshi, M., Matsuo, H., Maruo, T.: "Expression of Fas/Fas-Ligand, Bcl-2 Protein and Apoptosis in Extravillous Trophoblast along Invasion To the Decidua in Human Term Placenta."Endocrine Journal. Vol.50. 199-207 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shirosita, Y., Matsuo, H.: "The relationship between clinical characteristics And pregnancy outcome in preeclanpsia."J Perinat Med.. Vol.31. 194-194 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamanaka, Y., Matsuo, H., Nakago, S., Maruo, T.: "Effects of estriol on cell Vibility and 1,25-Dihydroxyvitamin D3 receptor mRNA expression in Cultured human osteoblast-like cells(HOS)."Gyynecol Endocrinl. Vol.17. 455-461 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Matsuo, H.: "Prediction of the change of bone mineral density induced By gonadotropin-releasing hormoneagonist treatment for endometriosis."Fertility Sterility. Vol.81. 149-153 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Y.Yamanaka, H.Matsuo, T.Maruo: "Effects of estriol on cell viability and 1,25-dihydroxyvitamin D3 receptor mRNA expression in cultured human osteoblast-like cells"Gynecol Endocrinol. 17. 455-461 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] HOMARE MURAKOSHI, HIROYA MATSUO, TAKESHI MARUO: "Expression of Fas/Fas-Ligand, Bcl2 Protein and Apoptosis in Extravillous Trophaoblast along Invasion to the Decidua in Human Term Placenta"Endocrine Journal. 50. 199-207 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shigeki Takekida, Hiroya Matsuo, Takeshi Maruo: "GnRH agonist action on granulose at varying follicular stages"Molecular and Cellular Endocrinology. 202. 155-164 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 安川 純代, 松尾 博哉: "包括的健康関連QOL尺度SF-36を指標いした更年期障害患者に対するホルモン補充療法の有用性の評価の試み"産婦の進歩. 55. 1-10 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hiroya Matsuo: "Prediction of the change in bone mineral density induced by gonadotropin-releasing hormone agonist treatment for endometriosis"FERTILTY AND STERILITY. 81. 149-153 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 松尾 博哉, 丸尾 猛: "子宮筋腫と性ステロイド"産婦人科治療. 86. 251-256 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Wang Y, Matsuo H, Gao Z, Nakago S, Maruo T: "Down-regulation of proliferation and up-regulation of apoptosis by gonadotropin-releasing hormone agonist in cultured uterine leiomyoma cells"Endocrine Journal Of Endocrinology. 146. 447-456 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ishihara N, Matsuo H, Murakoshi H, Fernandez J, Samoto T, Maruo T: "Increased apoptosis in the syncytiotrophoblast in human term placentas complicated by either preeclampsia or intrauterine growth retardation"Am J Obstet Gynecol. 186. 158-166 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Gao Z, Matsuo H, Wang Y, Nakago S, Maruo T: "p53 tumor suppressor protein content in human uterine leiomyomas and its down-regulation by 17β-estradiol"J Clin Endocrinol Metab. 87. 3915-3920 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Oki N, Matsuo H, Murakoshi H, Fernandez J, Maruo T: "Effect of 3,5,3'-triiodothyronine of cell adhesion molecule expression in cultured early placental extravillous trophoblast"Placenta. 23. A2 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 千々和真理, 松尾博哉, 丸尾猛: "低骨密度若年女性の骨代謝動態の特徴"日本更年期医学会雑誌. 10. 207-215 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 安川純代, 松尾博哉: "包括的健康関連QOL尺度SF-36を指標にした更年期障害患者に対するホルモン補充療法の有用性の評価の試み"産婦人科の進歩. 55. 1-10 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 松尾博哉, 丸尾猛: "婦人科検査マニュアル 内分泌機能検査-甲状腺ホルモン"医学書院(倉智智久). 5 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kurach O: "Tumor necrosis factor-α expression in human uterine leiomyoma and its down-regulation by progesterone"J Clin Endocrinol Metab. 86. 2275-2280 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Gao Z: "Up-regulation by insulin-like growth factor I of proliferaing cell nuclean antogen and Bcl-2 protein expression in human uterine leiomyoma cells"J Clin Endocrinol Metab. 86. 5593-5599 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Matsuo H: "Expreddion patterns of Fas/Fash, Bil-2 protein and apoptesis of extravillous trophoblast(EVT) along with the invasive pathway"Placenta. 22. A7 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Murakoshi H: "Increased apoptosis in the extravillous trophoblasts in human term placentas complicated by ceither preeclampsia or IUGR"Placenta. 22. A7 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 松尾博哉: "栄養代謝関連因子ならびにアポトーシス関連因子によるヒト絨毛細胞増殖能と機能発現の調節:3の絨毛病態への関わり"日本産科婦人科学会雑誌. 5・3. 1637-1647 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ishihara N: "Increased apoptosis in the syacytiotrophoblast in human term placentas complicated by either preeclampsia or IUGR"Am J obstet Gynecol. 186. 158-166 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 松尾博哉: "妊娠の生物学"永井書店. 396 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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