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Role of leptin and interleukin-8 in human ovary

Research Project

Project/Area Number 13671716
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionTottori University School of Medicine

Principal Investigator

IRIE Takashi  Tottori Univ., Dept.Obstet.Gunecol., Assistant Professor, 医学部附属病院, 講師 (70273894)

Co-Investigator(Kenkyū-buntansha) HARADA Tasuku  Tottori Univ., Dept.Obstet.Gunecol., Assistant Professor, 医学部, 講師 (40218649)
KIGAWA Junzo  Tottori Univ., Dept.Obstet.Gunecol., Associate Professor, 医学部, 助教授 (00177784)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsleptin / estrogen / granulosa cell / KGN cells / IL-8 / アポトーシス
Research Abstract

We evaluated the expression, regulation, and role of leptin and interleukin (IL-8) in human ovary. Follicular fluid and granulosa lutein cells (GLCs) were collected during IVF cycles. KGN cells, the human granulosa cell tumor cell line, were also used. The levels of leptin and IL-8 in follicular fluid and IL-8 protein production were determined using ELISA. The levels of leptin in follicular fluid showed a positive correlation with those of IL-8. Reverse transcription polymerase chain reaction showed the presence of leptin receptor mRNA and IL-8 mRNA in GLCs and KGN cells. In contrast, IL-8 receptor mRNA was not detected in these cells. The addition of E2 and FSH significantly increased the IL-8 protein production in KGN cells, whereas there were no effects on the IL-8 protein production. in GLCs. On the other hand, IL-8 protein production was not influenced by leptin in KGN cells and GCLs. The expression of IL-8 in GLCs and KGN cells was increased by addition of IL-1beta and TNFalpha. Interleukin-8 increased the proliferation of ovarian stromal cells. The expression of pIkappaB in KGN cells was induced by IL-1beta, and the effects were reduced by APDC. Interleukin 8 induced by IL-1beta via activation of NF-kappaB in granulosa cells may have a role in the periovulatory period of follicular maturation.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Taniguch F: "Hepatocyte growth factor promotes cell proliferation and inhibits progesterone secretion via PKA and MAPK pathways in a human granulosa cell line."Mol Reprod Dev. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Iwabe T: "Gonadotropin-releasing hormone agonist treatment reduced serum interleukin-6 concentrations in patients with ovarian endometriomas."Fertil Steril. 80. 300-304 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujii A: "Interleukin-8 gene and protein expression are up-regulated by interleukin-1 beta in normal human ovarian cells and a granulosa tumor cell line."Fertil Steril. 79. 151-157 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sato S: "Timing of G-CSF administration based on the circadian rhythm in patients with ovarian cancer."Am J Clin Oncol. 25. 289-290 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ito M: "Hepatocyte growth factor and stem cell factor involvement in paracrine interplays of theca and granulose cell in the human ovary."Fertil Steril. 75・5. 973-979 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 入江 隆: "臨床エビデンス婦人科学(肥満とやせ)"メディカルレビュー社. 699 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Taniguch F: "Hepatocyte growth factor promotes cell proliferation and inhibits progesterone secretion via PKA and MAPK pathways in a human granulosa cell line."Mol Reprod Dev.. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Iwabe T: "Gonadotropin-releasing hormone agonist treatment reduced serum interleukin-6 concentrations in patients with ovarian endometriomas."Fertil Steril.. 80(2). 300-304 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujii A: "Interleukin-8 gene and protein expression are up-regulated by interleukin-1beta in normal human ovarian cells and a granulosa tumor cell line."Fertil Steril.. 79(1). 151-157 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sato S: "Timing of G-CSF administration based on the circadian rhythm in patients with ovarian cancer."Am J Clin Oncol.. 25(3). 289-290 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ito M: "Hepatocyte growth factor and stem cell factor involvement in paracrine interplays of theca and granulose cell in the human ovary."Fertil Steril.. 75(5). 973-979 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Taniguch F: "Hepatocyte growth factor promotes cell proliferation and inhibits progesterone secretion via PKA and MAPK pathways in a human granulosa cell line."Mol Reprod Dev. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Iwabe T: "Gonadotropin-releasing hormone agonist treatment reduced serum interleukin-6 concentrations in patients with ovarian endometriomas."Fertil Steril. 80. 300-304 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 入江 隆: "臨床エビデンス婦人科学(肥満とやせ)"メディカルレビュー社. 699 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Fujii A: "Interleukin-8 gene and protein expression are up-regulated by interleukin-1beta in normal human ovarian cells and a granulosa tumor cell line"Fertil Steril. 79・1. 151-157 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ito M: "Hepatocyte growth factor and stem cell factor involvement In paracrine interplays of theca and granulose cell in the human ovary"Fertil Steril. 75・5. 973-979 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Masahiro T: "Presence of stem cell factor in follicular fluid and Its expression in the human ovary"Fertil Steril. 73・6. 1259-1260 (2000)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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