| Co-Investigator(Kenkyū-buntansha) |
SONODA Kenzo Department of Obstetrics and Gynecology, Graduate School of Medical Sciences, 医学部附属病院, 助手 (30294929)
FUKUSHIMA Kotaro Department of Obstetrics and Gynecology, Graduate School of Medical Sciences, 医学研究院, 助手 (40304779)
MIYAMOTO Shingo Department of Obstetrics and Gynecology, Graduate School of Medical Sciences, 医学部附属病院, 助手 (40209945)
HIRAKAWA Toshio Department of Obstetrics and Gynecology, Graduate School of Medical Sciences, 医学部附属病院, 講師 (20218770)
KOBAYASHI Hiroaki Department of Obstetrics and Gynecology, Graduate School of Medical Sciences, 医学部附属病院, 助手 (70260700)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Research Abstract |
To clarify the mechanism of canceration from trophoblastic disease to choriocarcinoma, we examined expressions of TNF-α, Rcas1, Fas Ligand in normal villi, hydatidiform mole, invasive mole, and choriocarcinoma. The results are as follows: (1) The expression of Fas Ligand, which appears in normal villi, is disappeared in circumferential proliferation of hydatidiform mole. (2) No expression of Fas Ligand in invasive mole and choricarcinoma. (3) No expression of TNF-α is observed in normal villi, hydatidiform mole, invasive mole, and choriocarcinoma. (4) No expression of Rcas1 is observed in normal villi, hydatidiform mole. (5) The expression of Rcas1 is observed in invasive mole and choriocarcinoma. These results suggested that apoptosis induced factors may involve in canceration from trophoblastic disease to choriocarcinoma.
Apoptosis induced factors, TNF-α, Rcas1, and Fas Ligand are secreted by shedding on cell membrane. The expressions of TNF-α, Rcas1, and Fas Ligand are controlled by
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G Protein Coupled Receptor (GRCR) on cell membrane. Edg2 receptor (endothelial differentiation gene -2 rec eptor), one of GRCR, which is observed high expression in chorionic villi, play a central role of angiogenesis. In view of the above, we try to investigate expressions of Edg2, Edg4, and Edg7 in normal villi, hydatidiform mole, invasive mole, and choriocarcinoma. Firstly to make clear about the biological significance of Edgs, we analyzed the alterations of Edg2, Edg4, and Edg7 from non-cancerous state to cancerous state. The results are as follows: (1) The expression of Edg2 is decreased and the expressions of Edg4 and Edg7 are increased from non-cancerous state to cancerous state. (2) We analyzed the correlation coefficiency in the expression between Edg2, Edg4, or Edg7 and angiogenetic factors, IL-8 or VEGF. A significant corr elation is found in the expression between Edg4 and VEGF (γ=0.613, P<0.0001). The correlation coefficient in the expression between Edg7 and VEGF (γ=0.434, P<0.001). These results suggested that signals mediated by Edgs may be involved in production of angiogenetic facto rs and cancer behavior. Less
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