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Study on expression profile for heterogeneiety in Japanese and Chinese ovarian cancer tissues

Research Project

Project/Area Number 13671751
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionJTKEI UNIVERSITY SCHOOL OF MEDICINE

Principal Investigator

OKAMOTO Aikou  JIKEI UNIVERSITY SCHOOL OF MEDICINE, Assistant professor, 医学部, 講師 (20204026)

Co-Investigator(Kenkyū-buntansha) OCHIAI Kazunori  JIKEI UNIVERSITY SCHOOL OF MEDICINE, Professor, 医学部, 教授 (20152514)
TAKANO Hirokani  JIKEI UNIVERSITY SCHOOL OF MEDICINE, Assistant, 医学部, 助手 (50226809)
TAKAKURA Satoshi  JIKEI UNIVERSITY SCHOOL OF MEDICINE, Assistant, 医学部, 助手 (60256401)
TANAKA Tadao  JIKEI UNIVERSITY SCHOOL OF MEDICINE, Professor, 医学部, 教授 (50110929)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥2,800,000 (Direct Cost: ¥2,800,000)
Keywordsovanan cancer / chemotherapy reistance / cDNA microanay / Laser capture microdissection (LMD) / cDNA マイクロアレイ / Laser Capture Microdissection (LMD) / 卵巣がん / Laser Capture Microdissection(LPM)
Research Abstract

We screened 500 genes to identify genes associated with chemotherapy resistance by cDNA array analysis in ovarian cancer surgical specimens, and 68 genes showing different expression with the degree of more than 4 times were selcted. Moreover, we established 2 kinds of Paclitaxel resistarit ovarian cancer cell lines derived an ovarian cancer cell lines named 2008, and screened 9121 genes by cDNA microarray. Three genes were selected by these analyses. We microdlssected cancerous lesion and ovarian surface epithelium using Laser capture microdissection (LMD), extracted total RNA, amplified RNA by T7 based amplification, and examined expressions of 3 genes by RT-PCR. Interestingly, basic transcription element binding protein (BTEB1) gene showed higher expression in Paplitaxel resistant surgical specimens. We transfected BTEB1 cDNA in ovarian cancer cell line, 2008, and confirmed the increase of resistance to Paclitaxel with the rate of more than 4 times. cDNA microarray analysis is being performed using this transfectant and 2008.
We established cDNA microarray method using LMD and T7 based amplification with single adaptor. Using this method, we examine the expression profiles by histological types, grades, staging, chemothrapy sensitivities. There are synchronous lesions including normal surface epithelium, adenoma, borderline malignancy, and cancer in some mucinous adenocarcinemas. These samples are ideal for clarify the mechanism of ovarian carcinogenesis. We have collected these samples, and LMD-cDNA microarray is being performed.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] 岡本愛光, 山本佳津恵, 斉藤美里, 磯西成治, 大竹康之, 落合和徳, 田中忠夫: "卵巣がんの抗がん剤耐性化機構に関与する遺伝子"Oncology & Chemotherapy. 17. 261-264 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 岡本愛光, 高野浩邦, 高倉聡, 磯西成治, 田中忠夫, 落合和徳: "卵巣癌の取り扱いUpdate 上皮性卵巣がんの遺伝子変化とその臨床応用への展望"日本婦人科腫瘍学会雑誌. 20. 47-58 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamamoto K, Okamoto A, Isonishi S, Ochiai K, Ohtake Y.: "A novel gene, CRR9, which was up-regulated in CDDP-resistant ovarian tumor cell line, was associated with apoptosis"Biochem Biophys Res Commun. 280. 1148-1154 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamamoto K, Okamoto A, Isonishi S, Ochiai K, Ohtake Y.: "Heat shock protein 27 was up-regulated in cisplatin resistant human ovaarian tumor cell line and associated with the cisplatin resistance"Cancer Letters. 168. 173-181 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shinozaki H, Okamoto A, Shimizu K, Saito M, Yokota J, Ochiai K.: "Absence of p51 alteration in human ovarian cancer"Int J Oncol. 18. 549-552 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takakura S, Kohno T, Manda R, Okamoto A, Tanaka T, Yokota J.: "Genetic alterations and expression of the protein phosphatase 1 genes in human cancers"Int. J. Oncol. 18. 817-824 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takaktira S, Kohno T, Manda R, Okamoto A. Tanaka T,and Yokota J.: "Genetic alterations and expression of the protein phosphatase 1 genes in human cancers."Int. J. Oncol. 18. 817-824 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shinozaki H, Okamoto A. Shimizu K, Saito M, Yokotk J, Ochiai K.: "Absence ofp51 alteration in human ovarian cancer."Int. J. Oncol. 41. 549-552 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamamoto K, Okamoto A. Isonishi S, Ochiai K, Ohtake Y.: "A noval gene, CRR9, which was up-regulated in CDDP-resistant ovarian tumor cell line, was associated with apoptosis."Biockem Biophys Res Commun. 280. 1148-1154 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamamoto K, Okamoto A, . Isonishi S, Ochiai K,Ohtake Y.: "Heat shock protein 27 was up-regulated in cisplatin resistant human ovarian tumor cell line and associated with the cisplatin resistance."Cancer Letters. 168. 173-181 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takakura S, Kohno T, Manda R, Okamoto A, Tanaka T, Yokota J: "Genetic alterations and expression of the protein phosphatase 1 genes in human cancers"Int. J. Oncol. 18. 817-824 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 岡本愛光, 山本佳津恵, 斉藤美里, 磯西成治, 大竹康之, 落合和徳, 田中忠夫: "卵巣がんの抗がん剤耐性化機構に関与する遺伝子"Oncology & Chemotherapy. 17. 261-264 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 岡本愛光, 高野浩邦, 高倉聡, 磯西成治, 田中忠夫, 落合和徳: "卵巣癌の取り扱いUpdate上皮性卵巣がんの遺伝子変化とその臨床応用への展望"日本婦人科腫瘍学会雑誌. 20. 47-58 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yamamoto K, Okamoto A, Isonishi S, Ochiai K, Ohtake Y.: "A novel gene, CRR9, which was up-regulated in CDDP-resistant ovarian tumor cell line, was associated with apoptosis"Biochem Biophys Res Commun. 280. 1148-54 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamamoto K, Okamoto A, Isonishi S, Ochiai K, Ohtake Y.: "Heat shock protein27 was up-regulated in cisplatin resistant human ovarian tumor cell line and associated with the cisplatin resistance"Cancer Letters. 168. 173-181 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shinozaki H, Okamoto A, Shimizu K, Saito M, Yokota J, Ochiai K.: "Absence of p51 alteration in human ovarian cancer"Int.J.Oncology. 18. 549-552 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ono K, Tanaka T, Tsunoda T, Kitahara O, Kihara C, Okamoto A, Ochiai K, Takagi T, Nakamura Y.: "Identification by cDNA microarray of genes involved in ovarian carcinogenesis"Cancer Res. 60. 5007-5011 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takakura S, Kohno T, Shimizu K, Ohwada S, Okamoto A, Yokota J.: "Somatic mutations and genetic polymorphisms of the PPP1R3 gene in patients with several types of cancers"Oncogene. 19. 836-840 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Saito M, Okamoto A, Kohno T, Takakura S, Shinozaki H, Isonishi S, Yasuhara T, Tanaka T, Yoshimura T, Ohtake Y, Ochiai K, Yokota J.: "Allelic imbalance and mutations of the PTEN gene in ovarian cancer"Int J Cancer. 85. 160-165 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kohno T, Takakura S, Yamada T, Okamoto A, Tanaka T, Yokota J.: "Alterations of the PPP1R3 gene in human cancer"Cancer Res. 59. 4170-4174 (1999)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takano H, Okamoto A, Fukushima K, Ochiai K, Tanaka T.: "Low specificity of cytokeratin 19 mRNA expression in the peripheral blood cells from patients with ovarian tumors"Oncol Rep. 7. 1023-1025 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tagashira M, Kitagawa T, Isonishi S, Okamoto A, Ochiai K, Ohtake Y.: "Mithramycin represses MDR1 gene expression in vitro, modulating multidrug resistance"Biol Pharm Bull. 23. 926-929 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tagashira M, Kitagawa T, Nozato N, Isonishi S, Okamoto A, Ochiai K, Ohtake Y.: "Two novel C-glycosides of aureolic acid repress transcription of the MDR1 gene"Chem Pharm Bull. 48. 575-578 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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