| Project/Area Number |
13671791
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Kagoshima University |
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Principal Investigator |
FUKUIWA Tatsuya Kagoshima University, Graduate School of Medical and Dental Sciences, Research Associate, 大学院・医歯学総合研究科, 助手 (60325785)
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| Co-Investigator(Kenkyū-buntansha) |
KURONO Yuichi Kagoshima University, Graduate School of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (80153427)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | mucosal immunology / intranasal administration / Haemophilus influenzae / adjuvant / mouse |
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| Research Abstract |
A new recombinant adjuvant derived from mutant CT A1 subunit (mCTA1) was developed and was fused with recombinant P6, one of the outer membrane proteins of nontypeable Haemophilus influenzae (NTHi) (mCTA1-P6). The effects of this antigen in inducing muosal immune responses by intranasal immunization were examined in mice.
Antigen-specific IgG antibodies in the serum were induced by intraperitoneal injection with 500ug of mCTA1-P6 and 500ug of recombinant P6 together with 10 ug of cholera toxin. ELISPOT assay showed that mononuclear cells producing specific IgG in the spleen were induced by mCTA1-P6 as well as recombinant P6 together with cholera toxin. These results indicated that mCTA1-P6 is effective to induce specific immune responses. From these findings 500ug of mCTA1-P6 and 500ug of rP6 together with 10ug of CT was used for intranasal immunization.
In intranasal immunization, ELISA showed that antigen-specific IgA antibodies were increased in nasal wash after immunization with mCTA1-P6 and rP6 together with CT. Specific IgG antibodies in serum were increased in both groups. ELISPOT assay showed that the number of IgG producing cells in spleen and IgA producing cells in submandibular glands and intestine were significantly increased after intranasal immunization with mCTA1-P6 and rP6 together with CT. Interestingly, the numbers of IgG producing cells were higher in intranasally immunized group than in intraperitoneally immunized group. Serum IgG1 antibody titers were significantly higher than IgG2a antibody titers in both groups. However, agglutination activities and antibody titers against whole cells of NTHi were not observed.
Those findings suggest that mCTA1-P6 might be one of the vaccine candidates for NTHi infection.
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