| Project/Area Number |
13671817
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | AKITA UNIVERSITY |
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Principal Investigator |
SAKURAGI Shozo Akita University School of Medicine, Proffesor, 医学部, 教授 (80006767)
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| Co-Investigator(Kenkyū-buntansha) |
KONNO Seiki Akita University School of Medicine Research Associate, 医学部, 助手 (00312710)
YAMAKI Kunihiko University School of Medicuie, Associate Proffesor, 医学部, 助教授 (20125751)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | VKH disease / Tyrosinase family proteins / T cell / T cell clone / animal model / Dendritic Cell / Tyrosinase related protein 1 / Animal model / Tyrosinase TRP1 / T cell clonl / 前房水 / auto reactive T cell |
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| Research Abstract |
We had established animal model of Vogt-Koyanai-Harada (VKH) disease. This model was induced by immunization of tyrosinase family proteins and the histologic findings of this disease was almost identical to that of human VKH disease.
We had done lymphocyte proliferation assay against tyrosinase family proteins using PBMC of acute and untreated stage of VKH disease patients. These lymphocytes showed proliferative response against one or more of the peptides derived from tyrosinase and/or TRP1. From these facts, we proposed that the target antigens of VKH disease are tyrosinase family proteins.
Then, we established T cell clone from PBMC of the VKH disease patients. The TCCs from VKH disease patients showed reactivity against the peptides that can interact with the HLA BRB1*04Q5. These TCCs were Thl cells and may play important role to the induction of the disease.
Finally, we established TCCs from aqueous humors and cerebrospinal fluid (CSF) of VKH disease patients. We could establish TCCs from 7 of the fresh, and untreated stage of VKH disease patients. Many TCCs were established and 30 clones, 21 from aqueous humor and 9 from CSF were tested the reactivity against tyrosinase family proteins. Surprisingly, 10/21 of TCCs from aqueous humor and 2/9 of TCCs from CSF showed proliferative response against tyrosinase or TRP1. These ratios were very high. We are now analyzing the T cell receptor (TCR) with RT-PCR SSCP. These methods may reveal the T cells that cause the autoimmune disease.
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