| Project/Area Number |
13671825
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SUZUKI Yasuyuki School of Medicine, Department of Ophthalmology, Lecturer, 医学部附属病院, 講師 (80196881)
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| Co-Investigator(Kenkyū-buntansha) |
KUNIMATSU Shiho School of Medicine, Department of Ophthalmology, research associate, 医学部附属病院, 助手 (80301563)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | Muller cell / hypoxia / high-pressure / neurotrophic factor / nitric oxide / nitric oxide synthetase / betaxolol / iganidipine / 網膜神経保護 / 高圧ストレス |
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| Research Abstract |
We investigated the neuroprotective function of Muller cell using rat cultured Muller cell. Muller cells from 3-day-old Wister rat were isolated and cultured with DMEM with 10% fetal bovine serum. Change of the mRNA and protein expression level of brain-derived neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF) and glial cell line-derived neurotrophic factor (GDNF) was examined under hypoxic stress, high-pressure stress, and applying several drugs. The expression level of mRNA and protein was determined by the reverse transcriptase-polymerase chain reaction (RT-PCR), real-time PCR and Western blot analysis. As for the high-pressure stress, its effect on nitric oxide (NO) production was also investigated. Under hypoxia or high-pressure stress, the expression level of BDNF, CNTF and GDNF mRNA was decreased. After removal of the stress, the expression level recovered gradually and finally increased compared with control. The expression level of NO synthetase-2 and NO production was increased under high-pressure stress. Betaxolol and iganidipine up-regulated BDNF, CNTF, and GDNF mRNA. Western blot analysis showed the BDNF and GDNF protein was also up-regulated. These observations suggest that Muller cells have influence on retinal ganglion cells by changing neurotrophic factor expression under several conditions. Some drugs were proved to be effective in up-regulating the neurotrophic factor in Muller cells, then may have neuroprotecting function for retinal ganglion cells.
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