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Trial of regeneration of retinal neuronal cells by myoseverin

Research Project

Project/Area Number 13671830
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionNagoya University

Principal Investigator

IWAMOTO Takashi  Nagoya University, Radioisotope Research Center, Researsh Associate, アイソトープ総合センター, 助手 (60223426)

Co-Investigator(Kenkyū-buntansha) MIYAKE Yozo  Nagoya University, Gradute School of Medicine, Professor, 医学系研究科, 教授 (30166136)
HOMAGUCHI Michinari  Nagoya University, Gradute School of Medicine, Professor, 医学系研究科, 教授 (90135351)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywordsmyoseverin / PC12 / retinal pigment epithelial cell / differentiation / regeneration / NGF / bFGF / 神経成長因子 / RalGDS
Research Abstract

Myoseverin is a microtubule-binding molecule that induces the dedifferentiation of C2C12 myotubes to individual muscle cells. Here we present the trial of the neural cell regeneration by myoseverin.
1) Rat pheochromocytoma PC12 cells differentiate to nerve cells by nerve growth factor (NGF). Addition of myoseverin did not promote of dedifferentiation of NGF-treated PC12 cells. However, when myoseverin was added simultaneously with NGF to PC12 cells, extension of axons was inhibited in about 15% cells. But they did not proliferate and finally exhibited apoptic cell death.
2) The induction of RalGDS has been known to inhibit the NGF-induced differentiation of PC12 cells. We found that myoseverin induced the mRNA of RalGDS in PC12 cells. Thus, RalGDS might play an important role in inhibiting NGF-induced differentiation.
3) We tried to clone the additional myoseverin-induced genes in PC12 cells and ribosomal PO gene, cofilin and GADD45β were cloned. We established the individual PC12 cell lines that stably expressed those genes and examined their response to NGF. Those cell lines, however, differentiated upon NGF stimulation as parental PC12 cells.
4) Retinal pigment epithelial (RPE) cells of rat embryo at day 12 of gestation were cultured in vitro. Basic fibroblast growth factor (bFGF) induced cell proliferation, suppressed their pigmentation, and increased the expression of CHX10 and nestin that were molecular markers of neural progenitors. Myoseverin enhanced these bFGF effects up to about 30%.
5) Although bFGF induced the expression of glial fibrillary acidic protein (GFAP) that was a molecular marker of Muller glial cells after 7 days culture, addition of myoseverin inhibited the induction.
Thus, while myoseverin has a cooperative effect on the transdifferentiation of RPE induced by bFGF during an initial stage, probably its toxicity inhibited their further differentiation to mature neural cells.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] TAKESHI SENGA: "Stat3-dependent induction of BATF in M1 mouse myeloid leukemia cells"Oncogene. 21. 8186-8191 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] TAKESHI SENGA: "LSSIG is a novel murine leukocyte-specific GPCR that is induced by the activation of STAT3"Blood. 101. 1185-1187 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] MAKOTO NAKAMURA: "Enhanced S-cone syndrome with subfoveal neovascularization"American Journal of Ophthalmology. 133. 575-577 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] MAKOTO NAKAMURA: "Incomplete congenital stationary night blindness associated with symmetrical retinal atrophy"American Journal of Ophthalmology. 134. 463-465 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] YOZO MIYAKE: "Establishment of the concept of new clinical entities--complete and incomplete form of congenital stationary night blindness"Nippon Ganka Gakkai Zasshi. 106. 737-755 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] TAKESHI SENGA: "Stat3-dependent induction of BATF in Ml mouse myeloid leukemia cells"Oncogene. 21. 8186-8191 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] TAKESHI SENGA: "LSSIG is a novel murine leukocyte-specific GPCR that is induced by the activation of STAT3"Blood. 101. 1185-1187 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] MAKOTO NAKAMURA: "Enhanced S-cone syndrome with subfoveal neovascularization"American Journal of Ophthalmology. 133. 575-577 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] MAKOTO NAKAMURA: "Incomplete congenital stationary night blindness associated with symmetrical retinal atrophy"American Journal of Ophthalmology. 134. 463-465 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] YOZO MIYAKE: "Establishment of the concept of new clinical entities-complete and incomplete form of congenital stationary night blindness"Nippon Ganka Gakkai Zasshi. 106. 737-755 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] TAKESHI SENGA: "Stat3-dependent induction of BATF in M1 mouse myeloid leukemia cells"Oncogene. 21. 8186-8191 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] TAKESHI SENGA: "LSSIG is a novel murine leukocyte-specific GPCR that is induced by the activation of STAT3"Blood. 101. 1185-1187 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] MAKOTO NAKAMURA: "Enhanced S-cone syndrome with subfoveal neovascularization"American Journal of Ophthalmology. 133. 575-577 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] MAKOTO NAKAMURA: "Incomplete congenital stationary night blindness associated with symmetrical retinal atrophy"American Journal of Ophthalmology. 134. 463-465 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] YOZO MIYAKE: "Establishment of the concept of new clinical entities--complete and incomplete form of congenital stationary night blindness"Nippon Ganka Gakkai Zasshi. 106. 737-755 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] TAKESHI SENGA: "Jak/Stat3-dependent activation of the RalGDS/Ral pathway in MI mouse myeloid leukemia cells"Journal of Biological Chemistry. 276(35). 32678-32681 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] TAKASHI IWAMOTO: "STAT and SMAD signaling in cancer"Histology and Histopathology. In press.

    • Related Report
      2001 Annual Research Report
  • [Publications] MAKOTO NAKAMURA: "Japanese X-linked juvenile retionoschisis : conflict of genotype with novel mutations in the XLRS1 gene"Archives of Ophthalmology. 119(10). 1553-1554 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] NAGAKO KONDO: "Acute idiopathic blind spot enlargement syndrome : prolonged retinal dysfunction revealed by multifocal electroretinogram technique"American Journal of Ophthalmology. 132(1). 126-128 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] YASUYOSHI SOHARA: "Hyaluronan Activates Cell Motility of v-Src-transformed Cells via Ras-Mitogen-activated Protein Kinase and Phosphoinositide 3-Kinase-Akt in a Tumor-specific manner"Molecular Biology of the Cell. 12(6). 1859-1868 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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