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MODULATION OF INTRACELLULAR DISTRIBUTION AND EXPRESSION OF INWARD RECTIFYING POTASSIUM CHANNEL IN RETINAL PIGMENT EPITHELIUM

Research Project

Project/Area Number 13671836
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionOSAKA UNIVERSITY

Principal Investigator

IKUNO Yasushi (2002-2003)  Osaka University Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (50294096)

日下 俊次 (2001)  大阪大学, 医学系研究科, 講師 (60260387)

Co-Investigator(Kenkyū-buntansha) MATSUSHITA Kenji  Osaka University Graduate School of Medicine, Medical Staff, 医学部附属病院, 医員(臨床研究)
OHJI Masahito  Osaka University Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (90252650)
生野 恭司  大阪大学, 医学系研究科, 助手 (50294096)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsRetinal pigment epithelium / Kir7.1 / K+ channel / Kir4.1 / Anchoring protein / Patch cramp method / Na+K+pump / Na+-K+チャンネル / K+チャンネル / 内向き整流性K+チャネル / バッチランプ法 / K+チャネル
Research Abstract

We investigated if Kir7.1 potassium channel in RPE cells. We first performed RT-PCR method to detect mRNA of Kir7.1, and found its expression in cultured RPE cells. We immunized rabbits and prepared polyclonal anti-Kir7.1 antibody. Using this antibody, we detected protein expression of Kir7.1 by western-blotting. We then investigated the localization of Kir7.1 in RPE cells. Immunohistochemistiry was performed, and we found Kir7.1 was localized at the foot of microvilli of the apical base.
We then investigated the localization of other Kir family including Kir4.1 and Kir5.1 in the retina using immunohistochemistry. Kir5.1 was localized in the Muller cells and a part of neural cells of the retina. Its localization in Muller cells was diffuse in the cell body. While, Kir4.1 was localized at the end foot or around the vessel of Muller cells. Immunoprecipitation of Kir4.1 presented Kir5.1, indicating that Kir4.1 and Kir5.1 forms heterogenic tetramer at the cell body and the distal side of Muller cells.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (47 results)

All Other

All Publications (47 results)

  • [Publications] Fujikado T., Ikuno Y et al.: "Reading ability after macular translocation surgery with 360-degree retinotomy."American Journal of Ophthalmology. 134. 849-856 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikuno Y, et al.: "Retinal glial cells stimulate microvascular pericyte proliferation via fibroblast growth factor and platelet-derived growth factor in vitro."Japanese Journal of Ophthalmology. 46. 413-418 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikuno Y, Ohji M et al.: "Sutureless contact lens ring system during vitrectomy."American Journal of Ophthalmology. 133. 847-848 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Matsushita K, et al.: "Intramolecular interaction of SUR2 subtypes for intracellular ADP-Induced differential control of K(ATP) channels."Circulation Research. 90. 554-561 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Zheng Y, Ikuno Y, Ohji M, et al.: "Involvement of rho-kinase pathway in contoractile activity of rabbit RPE cells in vivo and in vitro."Investigative Ophthalmology Visual Science. 45. 668-674 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikuno Y, et al.: "Early macular holes with retinoschisis in highly myopic eyes."American Journal of Ophthalmology. 136. 741-744 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikuno Y, et al.: "Optical coherence tomographic findings of macular holes and retinal detachment after vitrectomy in highly myopic eyes"American Journal of Ophthalmology. 136. 477-481 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakata K, Ohji M, Ikuno Y et al.: "Sub-retinal hemorrhage during internal limiting membrane peeling for a macular hole."Grafe's Archive for Clinical and Experimental Ophthalmology. 241. 582-584 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kusaka S, Ohji M, Ikuno Y et al.: "Macular hole secondary to fungal endophthalmitis."Archives of Ophthalmology. 121. 732-733 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Zheng Y, Ikuno Y, Ohji M et al.: "Platelet-derived growth factor receptor kinase inhibitor AG1295 and inhibition of experimental proliferative vitreoretinopathy"Japanese Journal of Ophthalmology. 47. 158-165 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sou R, Ohji M, Ikuno Y et al.: "Optical coherence tomographic evaluation of a surgically treated traumatic macular hole secondary to Nd:YAG laser injury."American Journal of Ophthalmology. 135. 537-539 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Chachin A, Matsushita K et al.: "Nateglinide, a D-phenylalanine derivative lacking either a sulfonylurea or benzamido moiety, specifically inhibits pancreatic beta-cell-type K (ATP) channels"Journal of Pharmacological Experimental Therapy. 1025-1032 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikuno Y et al.: "An in vivo gene therapy approach for experimental proliferative vitreoretinopathy using the truncated platelet-derived growth factor alpha receptor."Investigative Ophthalmology Visual Science. 43. 2406-2411 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikuno Y et al.: "PI3K and PLC-gamma play a central role in experimental PVR"Investigative Ophthalmology Visual Science. 43. 483-489 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohji M, Ikuno Y et al.: "Comparison of three techniques of foveal translocation in patients with subfoveal choroidal neovascularlization resulting from age-related macular degeneration."American Journal of Ophthalmology. 132. 888-896 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikuno Y et al.: "TGFbeta1-dependent contraction of fibroblasts is mediated by PDGFalpha receptor"Investigative Ophthalmology Visual Science. 43. 41-46 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Inanobe A, Matsushita K et al.: "Interaction between the RGS domain of RGS4 with G protein alpha subunits mediates the voltage-dependent relaxation of the G protein-gated potassium channel."Journal of Physiology. 535. 133-143 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kusaka S, Matsushita K et al.: "Functional Kir7.1 channels localized at the root of apical processes in rat retinal pigment epithelium."Journal of Physiology. 531. 123-136 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Zheng Y, Bando H, Ikuno Y, Oshima Y, Sawa M, Ohji M, Tano Y.: "Involvement of rho-kinase pathway in contractile activity of rabbit RPE cells in vivo and in vitro."Investigative Ophthalmology Visual Science. 45. 668-674 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikuno Y, Tano Y.: "Early macular holes with retinoschisis in highly myopic eyes."American Journal of Ophthalmology. 136. 741-744 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikuno Y, Sayanagi K, Oshima T, Gomi F, Kusaka S, Kamei M, Ohji M, Fujikado T, Tano Y.: "Optical coherence tomographic findings of macular holes and retinal detachment after vitrectomy in highly myopic eyes."American Journal of Ophthalmology. 136. 477-481 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kusaka S, Hayashi N, Ohji M, Ikuno Y, Gomi F, Tano Y.: "Macular hole secondary to fungal endophthalmitis."Archives of Ophthalmology. 121. 732-733 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nakata K, Ohji M, Ikuno Y, Kusaka S, Gomi F, Tano Y.: "Sub-retinal hemorrhage during internal limiting membrane peeling for a macular hole."Graefe's Archives for Clinical and Experimental Ophthalmology. 241. 582-584 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Zheng Y, Ikuno Y, Ohj M, Kusaka S, Jiang R, Cekic O, Sawa M, Tano Y.: "Platelet-derived growth factor receptor kinase inhibitor AG1295 and inhibition of experimental proliferative vitreoretinopathy."Japanese Journal of Ophthalmology. 47. 158-165 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sou R, Kusaka S, Ohji M, Gomi F, Ikuno Y, Tano Y.: "Optical coherence tomographic evaluation of a surgically treated traumatic macular hole secondary to Nd : YAG laser injury."American Journal of Ophthalmology. 135. 537-539 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Fujikado T, Asonuma S, Ohji M, Kusaka S, Hayashi A, Ikuno Y, Kamei M, Oda K, Tano Y.: "Reading ability after macular translocation surgery with 360-degree retinotomy."American Journal of Ophthalmology. 134. 849-856 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikuno Y, Hibino S, Bando H, Kawasaki Y, Nakamura T, Tano Y.: "Retinal glial cells stimulate microvascular pericyte proliferation via fibroblast growth factor and platelet-derived growth factor in vitro."Japanese Journal of Ophthalmology. 46. 413-418 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikuno Y, Ohji M, Kusaka S, Gomi F, Nakata K, Futamura H, Tano Y.: "Sutureless contact lens ring system during vitrectomy."American Journal of Ophthalmology. 133. 847-848 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohji M, Fujikado T, Kusaka S, Hay ash i A, Hosohata J, Ikuno Y, Sawa M, Kubota A, Hashida N, Tano Y.: "Comparison of three techniques of foveal translocation in patients with subfoveal choroidal neovascularization resulting from age-related macular degeneration."American Journal of Ophthalmology. 132. 888-896 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Chachin M, Yamada M, Fujita A, Matsuoka T, Matsushita K, Kurachi Y.: "Nateglinide, a D-phenylalanine derivative lacking either a sulfonylurea or benzamido moiety, specifically inhibits pancreatic beta-cell-type K(ATP) channels."Journal of Pharmacological Experimental Therapy. 304. 1025-1032 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Matsushita K, Kinoshita K, Matsuoka T, Fujita A, Fujikado T, Tano Y, Nakamura H, Kurachi Y.: "Intramolecular interaction of SUR2 subtypes for intracellular ADP-Induced differential control of K(ATP) channels."Circulation Research. 90. 554-561 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikuno Y, Kazlauskas A.: "An in vivo gene therapy approach for experimental proliferative vitreoretinopathy using the truncated platelet-derived growth factor alpha receptor."Investigative Ophthalmology Visual Science. 43. 2406-2411 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikuno Y, Leong FL, Kazlauskas A.: "PI3K and PLCgamma play a central role in experimental PVR."Investigative Ophthalmology Visual Science. 43. 483-489 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ikuno Y, Kazlauskas A.: "TGFbeta1-dependent contraction of fibroblasts is mediated by the PDGFalpha receptor."Investigative Ophthalmology Visual Science. 43. 41-46 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Inanobe A, Fujita S, Makino Y, Matsushita K, Ishii M, Chachin M, Kurachi Y.: "Interaction between the RGS domain of RGS4 with G protein alpha subunits mediates the voltage-dependent relaxation of the G protein-gated potassium channel."Journal of Physiology. 535. 133-143 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kusaka S, Inanobe A, Fujita A, Makino Y, Tanemoto M, Matsushita K, Tano Y, Kurachi Y.: "RFunctional Kir7.1 channels localized at the root of apical processes in rat retinal pigment epithelium."Journal of Physiology. 531. 27-36 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Matsushita K et al.: "Intramolecular interaction of SUR2 subtypes for intracellular ADP-Induced differential control of K(ATP) channels"Circulation Research. 90. 554-561 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Chanchin M, Matsushita K et al.: "Nateglinide, a D-phenylalanine derivative lacking either a sulfonylurea or benzamido moiety, specifically inhibits pancreatic beta-cell-type K(ATP) channel"Journal of Pharmacology Experimental Therapy. 304. 1025-1032 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Zheng Y, Ikuno Y et al.: "Platelet-derived growth factor receptor kinase inhibitor AG1295 and inhibition of experimental proliferative vitreoretinopathy"Japanese Journal of Ophthalmology. 47. 158-165 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ikuno Y et al.: "Optical coherence tomographic findings of macular holes and retinal detachment after vitrectomy in highly myopic eyes"American Journal of Ophthalmology. 136. 477-481 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ikuno Y et al.: "Early macular holes with retinoschisis in highly myopic eyes"American Journal of Ophthalmology. 136. 741-744 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kusaka S, Ikuno Y et al.: "Macular hole secondary to fungal endophthalmitis"Archives of Ophthalmology. 121. 732-733 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Fujikado T., Ikuno Y, et al.: "Reading ability after macular translocation surgery with 360-degree retinotomy"American Journal of Ophthalmology. 134. 849-856 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ikuno Y, et al.: "Retinal glial cells stimulate microvascular pericyte proliferation via fibroblast growth factor and platelet-derived growth factor in vitro"Japanese Journal of Ophthalmology. 46. 413-418 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ikuno Y, Ohji M, et al.: "Sutureless contact lens ring system during vitrectomy"American Journal of Ophthalmology. 133. 847-848 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Matsushita K, et al.: "Intramolecular interaction of SUR2 subtypes for intracellular ADP-Induced differential control of K(ATP) channels"Circulation Research. 90. 554-561 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kusaka S, et al.: "Functional Kir7.1-channels localized at the root of apical processes in rat retinal pigment epithelium"The Journal of Physiology(London). 531. 27-36 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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