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INVESTIGATION OF NEW MAMBRANE MUCIN PRODUCED BY THE OCULAR SURFACE EPITHELIUM

Research Project

Project/Area Number 13671838
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionOsaka University

Principal Investigator

WATANABE Hiroshi  Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (60252673)

Co-Investigator(Kenkyū-buntansha) NISHIDA Kohji  Osaka University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (40244610)
井上 幸次  大阪大学, 医学系研究科, 助教授 (10213183)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Keywordsmucin / cornea / conjunctiva / Mucin / 角膜上皮 / IFN-g
Research Abstract

MUC 1, 4 and 5AC have been reported to express in the cornea and the conjunctiva. There exists some trans - membrane mucins capable of maintaining tear film over the ocular surface epithelium. However, based on knock out studies in mice, there are other mucins than MUC 1, and MUC4 having the function. Recently, MUC16 has been reported and this MUC16 may have the function to maintain tear film over the ocular surface. By real-time PCR on reverse transcription products from RNA isolated from human corneal and conjunctival tissues, MUC16 mucin gene was determined. Further studies by in situ hybridization and immunohistochemistry disclosed MUC16 mRNA was localized to the apical cell layers of the cornea. MUC16 protein was obsertved in apical cells of the corneal stratified cells. Whether MUC1 and MUC16 mucins carry the H185 carbohydrate epitope was performed by one- or two-step immunoprecipitation assays and immunodepletion experiments followed by Western blot analysis. Immunofluorescence microscopy demonstrated exact colocalization of the MUC16 mucin and the H185 carbohydrate epitope in the cornea. Immunoprecipitated products recognized by the H185 antibody mucin was recognized by the antibody recognizing MUC16 and Immunoprecipitated MUC16 mucin was recognized by the H185 antibody. These studies showed that MUC16 mucin carries the H185 epitope. Immunodepletion with H185 antibody has no reactivity to the antibodies by MUC16. No cross-reactivity between immunoprecipitated MUC1 and the H185 antibody was disclosed. This study demonstrates that the membrane-associated mucin MUC16 is expressed by the corneal epithelium. Moreover, MUC16 carries the H185 carbohydrate epitope.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (7 results)

All 2003 2002 Other

All Journal Article (4 results) Publications (3 results)

  • [Journal Article] Diagnosing dry eye using a blue-free barrier filter2003

    • Author(s)
      Koh S, Watanabe H, et al.
    • Journal Title

      American Journal of Ophthalmology 136

      Pages: 513-519

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] 蛍光濾過フィルターを用いた角結膜フルオレセイン染色所見の有効な観察法2003

    • Author(s)
      坂東勝美, 西田幸二, 前田直之, 渡辺仁, 田野保雄
    • Journal Title

      日本眼科紀要 54

      Pages: 416-419

    • NAID

      10011438187

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Usefulness of a Fluorescence Barrier Filter For Fluorescein Staining of the Ocular Surface.2003

    • Author(s)
      Bando K, Nishida K, Maeda N, Watanabe H, Tano Y.
    • Journal Title

      Folia Ophthalmologica Japonica 54

      Pages: 416-419

    • NAID

      10011438187

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Journal Article] Significance of mucin on the ocular surface.2002

    • Author(s)
      Watanabe H.
    • Journal Title

      Cornea 21

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Koh S, Watanabe H, et al.: "Diagnosing dry eye using a blue-free barrier filter"American Journal of Ophthalmology. 136(3). 513-519 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Bessho K, Maeda N, Watanabe H et al.: "Fourier analysis of corneal astigmatic changes following photorefractive keratectomy"Seminar in Ophthalmology. 18(1). 23-28 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hayashi H, Watanabe H et al.: "Sclerotic scattering illumination during phototherapeutic keratectomy for better visualization of corneal opacities"American Journal of Ophthalmology. 135(4). 559-561 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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