• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Effect on optic nerve of silicone oil tamponade in rabbits

Research Project

Project/Area Number 13671842
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionNAGASAKI UNIVERSITY

Principal Investigator

GONG Huaqing (2002)  Dept. of Ophthalmology and Visual Sciences, Lecturer, 医学部附属病院, 講師 (70304938)

齋藤 了一 (2001)  長崎大学, 医学部・附属病院, 講師 (80253649)

Co-Investigator(Kenkyū-buntansha) AMEMIYA Tsugio  Dept. of Ophthalmology and Visual Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (60026862)
宮 華青  長崎大学, 医学部・附属病院, 講師 (70304938)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsSilicone oil Tamponade / energy dispersive Xray analysis / gas mediated vitreous compression / シリコンオイル / 眼内タンポナーゼ / 分析電子顕微鏡
Research Abstract

Purpose : Side effects after intravitreal use of silicone oil (SO) are not yet well defined and elucidated. The object of this study is to examine the influence and toxicity of silicone oil on the optic nerve after vitrectomy with SO tamponade. Methods : We injected medical grade SO and emulsified SO into rabbit eyes after gas-mediated vitreous compression and examined them by light microscopy (LM), transmission electron microscopy (TEM), and energy dispersive X-ray analysis (EDXA) (point analysis and area analysis) 6 months after injection. We compared the findings in the non-treated eyes and eyes with only gas-mediated vitreous compression with those in SO-injected eyes. Results : Vacuole-like structures were seen in the optic nerve posterior to the lamina cribrosa. In group G the myelin structures were shown by TEM to be destroyed and replaced by glial tissue, while in groups M6 and E6, severe destruction of the myelin sheath (myelinolysis) was observed Silicone was identified at the electron dense edges of the vacuoles by EDXA point analysis, but not in the vacuoles without electron dense deposits. Dots of Si K alpha were not seen in the control groups, and dense dots were observed in SO injected groups, by EDXA area analysis. Conclusions : Some of the vacuoles might be artifacts caused by insufficient fixation or the operative procedure, but TEM showed almost no artifacts in the contrl optic nerve. Thus, most vacuoles may be SO storage sites. SO uptake into the optic nerve might play a role in the pathogenesis of optic nerve atrophy after SO injection.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] 齋藤了一: "Long-term effect on optic nerve of silicone oil tamponade in rabbits : histological and EDXA findings"Eye. 16. 171-176 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A Saitoh: "Long-term effect on optic nerve of silicone oil tamponade in rabbits ; histological and EDXA findings"Eye. 16. 171-176 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 齋藤了一: "Long-term effect on optic nerve of silicone oil temponade in rabbits : histological and EDXA findings"Eye. 16. 171-176 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] A.SAITOH, H.TANIGUCHI, H.GONG, A.OHIRA, T.AMEMIYA: "Long-term effect on optic nerve of silicone oil tamponade in rabbits ; histological and EDXA findings"(印刷中). (2002)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi