• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Basic Research of Photodynamic Therapy on Vascular Endothelial Cells.

Research Project

Project/Area Number 13671849
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionOsaka City University

Principal Investigator

OBANA Akira  Department of Ophthalmology and Visual Sciences, Osaka City University Graduate School of Medicine, Associate Professor, 大学院・医学研究科, 助教授 (40194625)

Co-Investigator(Kenkyū-buntansha) NAKAJIMA Susumu  Health Care Administration Center, Obihiro University of Agriculture and Veterinary Medicine, Professor, 保健管理センター, 教授 (70091577)
GOHTO Yuko  Department of Ophthalmology and Visual Sciences, Osaka City University Graduate School of Medicine, Research Associate, 大学院・医学研究科, 助手 (80305630)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2002: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
KeywordsPhotodvnamic Therapy / ATX-S10 / Verteporfin / Vascular Endothelial Cell / Retinal Pigment Epithelial Cell / Apoptosis / Choroidal Neovascularization / Age-related Macular Degeneration / ネクローシス / 培養内皮細胞 / 培養網膜色素上皮細胞 / 細胞内局在部位 / 殺細胞効果
Research Abstract

Phototoxicity of photodynamic therapy (PDT) using ATX-S10(Na) (ATX) and Verteporfin (BPD) was evaluated by MTS Assay on vascular endothelial cells and retinal pigment epithelial cells. PDT was conducted under two kinds of treatment conditions, short dye exposure PDT in which the cells were exposed to each photosensitizer for 5 minutes followed by laser irradiation without washing out the photosensitizer in the medium, and long dye exposure PDT, in which the cells were exposed to photosensitizers for more than 60 minutes, washed with phosphate-buffered saline, followed by laser irradiation using fresh medium. A diode laser of 670 nm wavelength was used for ATX-S10(Na) excitation and that of 689 nm for BPD.
The degree of phototoxicity depended on the dye concentration and radiant exposure. In short-exposure PDT on human microvascular endothelial cells (CryoHMVEC-Ad) with a laser dose of 50 J/cm^2, the ED_<90> was 6.3 μg/ml of ATX-S10(Na) and 0.04 μg/ml of BPD, while in long-exposure PDT t … More he ED_<90> was 50.0 μg/ml of ATX and 0.04 μg/ml of BPD. Laser and dye doses to obtain ED_<90> became higher when the medium was supplemented with higher concentration of serum. In short-exposure PDT, the ED_<90> of monkey choroid-retina vascular endothelial cells (CRL-1780) was 100 μg/ml of ATX-S10(Na) and 0.08 μg/ml of BPD and an radiance of 100 J/cm^2. In comparison of phototoxicity on human retinal pigment epithelial cells (ARPE-19) and vascular endothelial cells, phototoxicity on vascular endothelial cells was significantly higher than that on the retinal pigment epithelial cells with short-exposure PDT using ATX, however, long-exposure PDT with ATX and short- and long-exposure PDT with BPD failed to obtain higher phototoxicity on vascular endothelial cells than on the retinal pigment epithelial cells. The uptake aid subcellular localization of ATX and BPD was examined by measuring fluorescence of each dye. ATX was mainly localized in the lysosomes and BPD in cytoplasma diffusely.
Therefore, BPD had a higher phototoxicity than ATX. Some reasons were speculated for high phototoxibly of BPD, such as different amount and speed of uptake of the photosensitizer into the cells. In short-exposure PDT with ATX, vascular endothelial cells were selectively injured without damage on the retinal pigment epithelial cells, in contrast, with other PDT conditions, it seemed difficult to destroy vascular endothelial cells without damage on the retinal pigment epithelial cells since some dye was taken up into the retinal pigment epithelial cells.
The mode of cell death by PDT was observed by TUNEL staining and a laser scanning cytometer. Short-exposure PDT with ATX predominantly induced necrosis, while long-exposure PDT resulted in apoptosis. Therefore, the dye that located on or surrounding the cell membrane seems to predominantly induced necrosis of the target cell, while the dye that accumulated in lysosomes predominantly induced apoptosis.
ATX-S10 had a phototoxic effect on human skin malignant melanoma cells. Most of the dead cells appeared apoptotic with weak treatment conditions that induced low cytotoxicity. In contrast, most of them appeared necrotic with treatment conditions that induced more than 90% cytotoxicity. Less

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (35 results)

All Other

All Publications (35 results)

  • [Publications] Satosi Nagata, Akira Obana, Yuko Gohto, Susumu Nakajima: "Necrotic and apoptotic cell death of human malignant melanoma cells following photodynamic therapy using an amphiphilic photosensitizer, ATX-S10(Na)"Lasers in Surgery and Medicine. (印刷中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 尾花明, 郷渡有子, 中島進, 阪田功, 宮木末雄: "眼科領域における光線力学療法"レーザー学会学術講演解第22年次大会講演予稿集. 219-220 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 郷渡有子, 尾花明, 中島進: "原価における新しいレーザー医療-最新技術を応用した診断と治療法の紹介-"日本レーザー医学会誌. 22. 200 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Akira Obana, Yuko Gohto, Susumu Nakajima: "Subcellular localization of three photosensitizers in vascular endothelial cells"Clinical and Basic Applications of Photodynamic Medicine. IPA 8^<th> World Congress of Photodynamic Medicine. Abstract. 66 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yuko Gohto, Akira Obana, Yufang Huang, Susumu Nakajima: "In Vitro Photodynamic Effects of ATX-S10(Na) and Mode of Death of Vascular Endothelial Cells"Clinical and Basic Applications of Photodynamic Medicine. IPA 8^<th> World Congress of Photodynamic Medicine. Abstract. 66 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yuko Gohto, Akira Obana, Yufang Huang, Susumu Nakajima: "In vitro photodynamic effects of ATX-S10(Na) and mode of death of vascular endothelial cells"Investigative Ophthalmology and Visual Science. 42. 436 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Akira Obana, Yuko Gohto, Susumu Nakajima: "Subcellular localization of three different photosensitizers in vascular endothelial cells"Investigative Ophthalmology and Visual Science. 42. 437 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Akira Obana, Yuko Gohto, Susumu Nakajima: "Subcellular localization of ATX-S10(Na) in vascular endothelial cells"14^<th> World Congress of The International Society for Laser Surgery and Medicine. (CD-R). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 尾花 明: "PDTハンドブック 光線力学療法のアドバンストテクニック 6.加齢黄斑変性に対するPDTの適応と実際"医学書院、監修加藤治文、編集奥仲哲弥. 101 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Akira Obana: "Photodyanmic therapy of ocular diseases. PDT for experimental chroidal neovascularization using ATX-S10(Na)"Lippincott Williams & Wilkins, ed. ES Gragoudas, JW Miller, L Zografos(編集中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Satosi Nagata, Akira Obana, Yuko Gohto, Susumu Nakajima: "Necrotic and apoptotic cell death of human malignant melanoma cells following photodynamic therapy using an amphiphilic photosensitizer, ATX-S10(Na)"Lasers in Surgery and Medicine. on print. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Akira Obana, Yuko Gohto, Susumu Nakajima, Isao Sakata, Sueo Miyaki: "Photodynamic therapy in Ophthalmology"Proceedings of 22th Congress of Laser Association. 219-220 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yuko Gohto, Akira Obana, Susumu Nakajima: "New Laser Midicine in Ophthalmology. Diagnosis and Treatment by Modern Technology"Journal of Japan Society for Laser Surgery and Medicine. 22. 200 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Akira Obana, Yuko Gohto, Susumu Nakajima: "Subcellular localization of three photosensitizers in vascular endothelial cells"Clinical and Basic Applications of Photodynamic Medicine. IPA 8th World Congress of Photodynamic Medicine. Abstract:66. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yuko Gohto, Akira Obana, Yufang Huang, Susumu Nakajima: "In Vitro Photodynamic Effects of ATX-S10(Na) and Mode of Death of Vascular Endothelial Cells"Clinical and Basic Applications of Photodynamic Medicine. IPA 8th World Congress of Photodynamic Medicine. Abstract:66.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yuko Gohto, Akira Obana, Yufang Huang, Susumu Nakajima: "In vitro photodynamic effects of ATX-S10(Na) and mode of cell death on vascular endothelial cells"Investigative Ophthalmology and Visual Science. 42. 436 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Akira Obana, Yuko Gohto, Susumu Nakajima: "Subcellular localization of three different photosensitizaers in vascular endothelial cells"Investigative Ophthalmology and Visual Science. 42. 437 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Akira Obana, Yuko Gohto, Susumu Nakajima: "Subcellular localization of ATX-S10(Na) in vascular endothelial cells"14th World Congress of The International Society for Laser Surgery and Medicine. CD-R. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Akira Obana: "Application and Practice of PDT on Age-related Macular Degeneration, PDT Handbook. Advanced Technique of Photodynamic Therapy, Ed by Hirohumi Kato, Tetsuya Okunaka"Igaku-Shoin. 75-83 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Akira Obana: "6. PDT for experimental choroidal neovascularization using ATX-S10(Na), Photodynamic therapy of ocular diseases, Ed by ES Gragoudas, JW Miller, L Zografos"Lippincott Williams & Wilkins. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Satosi Nagata, Akira Obana, Yuko Gohto, Susumu Nakajima: "Necrotic and apoptotic cell death of human malignant melanoma cells following photodynamic therapy using an amphiphilic photosensitizer, ATX-S10(Na)"Lasers in Surgery and Medicine. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Akira Obana, Yuko Gohto: "Scanning laser system for photodynamic therapy of choroidal neovascularization"Lasers in Surgery and Medicine. 30. 1-6 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 尾花明, 郷渡有子, 中島進, 阪田功, 宮木末雄: "眼科領域における光線力学療法"レーザー学会学術講演会第22年次大会講演予稿集. 219-220 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 郷渡有子, 尾花明, 中島進: "眼科における新しいレーザー医療-最新技術を応用した診断と治療法の紹介-"日本レーザー医学会誌. 22. 200 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Akira Obana, Yuko Gohto, Susumu Nakajima: "Subcellular localization of three photosensitizers in vascular endothelial cells"Clinical and Basic Applications of Photodynamic Medicine. IPA 8^<th> World Congress of Photodynamic Medicine. Abstract. 66 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yuko Gohto, Akira Obana, Yufang Huang, Susumu Nakajima: "In Vitro Photodynamic Effects of ATX-S10(Na) and Mode of Death of Vascular Endothelial Cells"Clinical and Basic Applications of Photodynamic Medicine. IPA 8^<th> World Congress of Photodynamic-Medicine. Abstract. 66 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yuko Gohto, Akira Obana, Yufang Huang, Susumu Nakajima: "In vitro photodynamic effects of ATX-S10(Na) and mode of cell death on vascular endothelial cells"Investigative Ophthalmology and Visual Science. 42. 436 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Akira Obana, Yuko Gohto, Susumu Nakajima: "Subcellular localization of three different photosensitizaers in vascular endothelial cells"Investigative Ophthalmology and Visual Science. 42. 437 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Akira Obana, Yuko Gohto, Susumu Nakajima: "Subcellular localization of ATX-S10(Na) in vascular endothelial cells"14^<th> World Congress of The International Society for Laser Surgery and Medicine. (CD-R). (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 尾花 明: "PDTハンドブック光線力学療法のアドバンストテクニック 6.加齢黄斑変性に対するPDTの適応と実際"医学書院(監修加藤治文, 編集奥仲哲弥). 101 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Akira Obana: "Photodynamic therapy of ocular diseases. PDT for experimental choroidal neovascularization using ATX-S10(Na)"Lippincott Williams & Wilkins, ed. ES Gragoudas, JW Miller, L Zografos (編集印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 尾花明, 郷渡有子, 中島進, 阪田功, 宮木末雄: "眼科領域における光線力学療法"レーザー学会学術講演会第22年次大会講演予稿集. 219-220 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Akira Obana, Yuko Gohto, Susumu Nakajima: "Subcellular localization of ATX-S10(Na) in vascular endothelial cells."14^<th> World Congress of The International Society for Laser Surgery and Medicine. (CD-R). (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Akira Obana, Yuko Gohto, Susumu Nakajima: "Subcellular localizatin of three photosensitizers in vascular endothelial cells"Clinical and Basic Applications of Photodynamic Medicine. IPA 8^<th> World Congress of Photodynamic Medicine. Abstract. 66 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yuko Gohto, Akira Obana, Yufang Huang, Susumu Nakajima: "In Vitro Photodynamic Effects of ATX-S10(Na)and Mode of Death of Vascular Endothelial Cells"Clinical and Basic Applications of Photodynamic Medicine. IPA 8^<th> World Congress of Photodynamic Medicine. Abstract. 66 (2001)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi