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Development of improving method for respiratory function in newborn diaphragma hernia

Research Project

Project/Area Number 13671863
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatric surgery
Research InstitutionYAMAGATA UNIVERSITY

Principal Investigator

YAMAGIWA Iwao  Yamagata Univ, Med, Assistant Professor, 医学部, 助教授 (60182424)

Co-Investigator(Kenkyū-buntansha) SHIMAZAKI Yasuhisa  Yamagata Univ, Med, Professor, 医学部, 教授 (60116043)
INUI Kiyoshige  Yamagata Univ, Med, Assistant Professor, 医学部, 講師 (70250941)
渡辺 隆夫  山形大学, 医学部, 助教授 (60138922)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥2,600,000 (Direct Cost: ¥2,600,000)
Keywordsdiaphragma hernia / KGF / cell transplantation
Research Abstract

Animal model of diaphragmatic hernia was introduced by administration of nitrofen. Type II alveolar cell was transfected with plasmid encoding HGF, or KGF gene by lipofection method. The efficacy of transfection was 10-20%. The excretion of introduced proteins from cells in the culture was recognized by SDS PAGE. Rats were anestetized by pentobarbital and intratracheal intubarion was carried out. 1 x 10^<-6> cells transfected with specific genes were injected from periferal veins. These cells were trapped in the microcirculation of lung tissue. Cells were survived in the lung tissue. Rats of diaphragmatic hernia model were used for studying the efficacy of the cell transplantation from peripheral vein. KGF transfected cells improved the saturation of rats after injection from the veins.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

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