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Experimental and clinical research for effect of fetal anesthesia to neonatal stress response

Research Project

Project/Area Number 13671869
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatric surgery
Research InstitutionKyushu University

Principal Investigator

YAMANOUCHI Takeshi  Kyushu University, Faculty of medicine, Ass., 医学部付属病院, 助手 (50294921)

Co-Investigator(Kenkyū-buntansha) MASUMOTO Kouji  Kyushu University, Faculty of medicine, Ass., 医学部付属病院, 助手 (20343329)
TAGUCHI Tomoaki  Kyushu University, Faculty of medicine, Ass.Prof., 医学部付属病院, 助教授 (20197247)
SUITA Sachiyo  Kyushu University, Faculty of medicine, Prof., 大学院・医学研究院, 教授 (30038856)
UESUGI Toru  Kyushu University, Faculty of medicine, The medical stuff, 医学部付属病院, 医員
NAKAMURA Masatoshi  Kyushu University, Faculty of medicine, The medical stuff, 医学部付属病院, 医員
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordsfetal anesthesia / stress response / cytokine / cortisol / congenital diaphragmatic hernia / neonatal surgery / mechanical ventilation
Research Abstract

<Experimental research>
Neonatal rats in fetal anesthesia group (group FA) received an intra-abdominal injection with morphine hydrochloride (3mg/kg) to their mothers before caesarean section. Neonatal rats in control group (group C) received no morphine hydrochloride treatment. Caesarean sections were performed under general anesthesia with ether and pentobarbital sodium in both groups. Neonatal rats were intubated and managed under mechanical ventilation for 3 hours. Blood samples and bronchoalveolar lavage fluid (BAL) were collected at birth and 3 hours after birth. Serum levels of IL-6 and IL-10 and TNF-α levels of BAL were measured by ELISA.
The levels of serum IL-6 were not different between two groups at birth (74.1 ± 19.8 pg/ml in group C vs 81.7±46.5pg/ml in group FA), however they were significantly elevated in group C 3 hours after birth than those in group FA (183.0 ± 66.5 pg/ml vs 86.4 ± 46.8pg/ml respectively). The levels of serum IL-10 were not different between two groups … More . TNF-α concentrations in BAL at birth were elevated in group C than group FA, however the difference were not significant.
<Clinical research>
Neonates in group FA were consisted of CDH patients who received fetal anesthesia by giving morphine hydrochloride (20-30mg) to their mothers before caesarean section and were repaired within 24 hours after birth. Neonates in group non-FA consisted of surgical neonates born by caesarean section without fetal anesthesia and operated within 24 hours after birth. The degree of surgical stress evaluated by Oxford Surgical Stress Scale were not different between two groups. Measured parameters were the perioperative levels of serum CRP, cortisol and IL-6.
Serum CRP levels in group non-FA reached the peak value (23.68 ± 0.84 mg/dl) on postoperative day 2, however the peak value (0.77 ± 0.21 mg/dl) in group FA was significantly lower than that in group FA. Serum cortisol levels in group non-FA reached the peak value (25.0 ± 2.5 μg/dl) just after surgery and decreased in six hours, however serum cortisol levels in group FA were not elevated during study period. The serum levels of IL-6 in group non-FA showed biphasic peaks just after operations and 12 hours after operations, however serum IL-6 levels in group FA was not elevated after operations. Stress response induced by neonatal surgery were suppressed by fetal anesthesia. Less

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Nakamura M, Suita S, Yamanouchi T, Masumoto K, Ogita K, Taguchi S, Uesugi T: "Cortisol and cytokine responses after surgery in different age groups of pediatric patients"Pediatric Surgery International. 19(3). 194-199 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Suita S, Yamanouchi T, Masumot K, Ogita K, Nakamura M, Taguchi S.: "Changing profile of parenteral nutrition in pediatric surgery : a 30-year experience at one institute"Surgery. 131(1Suppl). S275-S282 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Suita S.: "Mass screening for neuroblastoma in Japan : lessons learned and future directions"Journal of Pediatric Surgery. 37(7). 949-954 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Taguchi T, Suita S, Ohkubo K, Ono J.: "Mutations in the sulfonylurea receptor gene in relation to the long-term outcome of persistent hyperinsulinemic hypoglycemia of infancy"Journal of Pediatric Surgery. 37(4). 593-598 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Taguchi T, Yamada T, Nakao M, Ogita K, Nishimot Y, Suita S.: "Cytokine response after segmental small intestinal transplantation : comparison of jejunal and ileal grafts"Transplantation Proceedings. 34(3). 1052 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 山内 健, 荻田桂子, 中村晶俊, 田口匠平, 上杉 達, 高田斉人, 水田祥代: "小児における術後の細胞外液量の評価に対するMEBIAの有用性の検討"栄養-評価と治療. 20(6). 73-78 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 山内 健, 水田祥代: "コメディカルのための静脈・経腸栄養手技マニュアル 3-II 小児に対する静脈栄養の特徴と実際(本郷充彦 日本静脈経腸栄養学会編)"株式会社 南江堂. 106-133 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakamura M, Suita S, Yamanouchi T, Masumoto K, Ogita K, Taguchi S, Uesugi T.: "Cortisol and cytokine responses after surgery in different age groups of pediatric patients"Pediatric Surgery International. 19(3). 194-199 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Suita S, Yamanouchi T, Masumtoo K, Ogita K, nakamura M, Taguchi S.: "Changing profile of parenteral nutrition in pediatric surgery : a 30-year experience at one institute"Surgery. 131(1,Suppl). S275-S282 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Suita S.: "Mass screening for neuroblastoma in Japan : lessons learned and future directions"Journal of Pediatr Surgery. 37(7). 949-954 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Taguchi T, Suita S, Okubo K, Ono J.: "Mutations in the sulfonylurea receptor gene in relation to the long-term outcome of persistent hyperinsulinemic hypoglycemia of infancy"Journal of Pediatr Surgery. 37(4). 593-598 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Taguchi T, Yamada T, Nakao M, Ogita K, Nishimoto Y, Suita S.: "Cytokine response after segmental small intestinal transplantation : comparison of jejunal and ileal grafts"Transplant Proceeding. 34(3). 1052 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakamura M, Suita S, Yamanouchi T, Masumoto K, Ogita K, Taguchi S, Uesugi T: "Cortisol and cytokine responses after surgery in different age groups of pediatric patients"Pediatric Surgery International. 19(In press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Suita S, Yamanouchi T, Masumoto K, Ogita K, Nakamura M, Taguchi S.: "Changing profile of parenteral nutrition in pediatric surgery : a 30-year experience at one institute"Surgery. 131(1 Suppl). S275-S282 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Suita S.: "Mass screening for neuroblastoma in Japan : lessons learned and future directions"Journal of Pediatr Surgery. 37(7). 949-954 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Taguchi T, Suita S, Ohkubo K, Ono J.: "Mutations in the sulfonylurea receptor gene in relation to the long-term outcome of persistent hyperinsulinemic hypoglycemia of infancy"Journal of Pediatr Surgery. 37(4). 593-598 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Taguchi T, Yamada T, Nakao M, Ogita K, Nishimoto Y, Suita S.: "Cytokine response after segmental small intestinal transplantation comparison of jejunal and ileal grafts"Transplant Proceeding. 34(3). 1052 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 水田祥代, 山内 健: "静脈栄養の実際-外科的疾患"周産期医学. 32(11). 1512-1516 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 山内 健, 水田祥代: "コメディカルのための静脈・経腸栄養手技マニュアル 3-II 小児に対する静脈栄養の特徴と実際"株式会社 南江堂、本郷充彦 日本静脈経腸栄養学会編. 27 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Suita S, Yamanouchi T, Masumoto K, Ogita K, Nakamura M, Taguchi S.: "Changing profile of parenteral nutrition in pediatric surgery : A 30-year experience at one institute"Surgery. 131. S275-S282 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Taguchi T, Suita S.: "Segmental small-intestinal transplantation : A comparison of jejunal and ileal grafts"Surgery. 131. S294-S300 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakamura M, Suita S, Yamanouchi T, Kubota M, Shono K, Ogita K, Taguchi S: "Effect of Liver Transplantation on Nutritional Status in Patients with Biliary Atresia. -How to Assess ? -"Asian Journal of Surgery. 24. 347-352 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 山内 健, 水田祥代, Tibboel D: "新生児,乳児における腸粘膜Potential Differenceを用いた腸管機能評価"静脈・経腸栄養. 16(2). 61-66 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 山内 健, 水田祥代: "侵襲期の栄養アセスメント-小児手術"栄養・評価と治療. 18(3). 403-408 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 中村晶俊, 山内 健, 田口智章, 荻田桂子, 水田祥代: "先天性横隔膜ヘルニアに合併した新生児遷延性肺高血圧症に対しPohsphodiesterase III阻害薬が有効であった一例"日小外会誌. 37. 1073-1077 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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