• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Regulation of osteoprotegerin localization and expression in bone remodeling

Research Project

Project/Area Number 13671903
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionOkayama University

Principal Investigator

NAKAMURA Hiroaki  Graduate School of Medicine and Dentistry, Associate Professor, 大学院・医歯学総合研究科, 助教授 (50227930)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsOsteoprotegerin / Osteoclasts / Osteoblasts / Bone Remodeling / Proteoglycan / Immunohistochemistry / セメントライン
Research Abstract

Osteoprotegerin (OPG), a soluble member of the tumor necrosis factor receptor family, works as a decoy by binding to receptor activator of nuclear factor (NF)-kB ligand (RANKL) and thus inhibits osteoclastogenesis by interrupting RANKL-mediated signaling. We investigated the localization of OPG in rat tibia using a specific peptide antibody and its expression by in situ hybridization in order to clarify the role of OPG in bone remodeling. Western blotting showed that the antibody reacted with a 90 kD band in lysate from rat tibiae. Immunohistochemical observation revealed that OPG reactivity was mainly seen on bone surfaces under osteoclasts. In bone matrices, OPG was also localized on cartilage/bone interfaces and cement lines. However, labeling was hardly detected in the region of contact between osteoclasts and stromal cells. Some osteoblasts and osteocytes showed weak labeling. These findings suggest that OPG derived from osteoblast-lineage cells and/or serum may be concentrated on resorbed bone surfaces, and subsequently on cement lines. OPG may play an important role in the prevention of excess bone resorption by inhibiting differentiation and activity of osteoclasts in bone remodeling. Since OPG has high affinity for heparin, we also compared the localization of proteoglycans, such as hyaluronate, heparan sulfate, keratan sulfate and chondroitin sulfate proteoglycans. However, their patterns are different from OPG. The mechanism of OPG localization in bone matrix should be addressed in future research.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Nakamura H.et al.: "Immunolocalization of keratan sulfate proteoglycan in rat calvaria"Arch.Histol.Cytol.. 64(1). 111-120 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takeyama M.et al.: "Immunohistochemical demonstration or cathepsin B and L in periodontal ligament(PDL) of the rat molar"Jpn J.Oral Biol.. 43(2). 194-202 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Khairul Matin et al.: "Recombinant human bone morphogenetic protein-2 impact on residual ridge resortpion after tooth extraction. An experimental study in rat"Int.J.Oral Maxillofac.Implants. 16(3). 400-411 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamamoto T.et al.: "Ultracytochemical study of medullary bone calcification in estrogen injected male Japanese quail"Anat.Rec.. 264(1). 25-31 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ueno T.et al.: "Cellular origin of endochondral ossification from grafted periosteum"Anat.Rec.. 264. 348-357 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakamura H.et al.: "Localization of osteoprotegerin(OPG) on bone surfaces and cement lines in rat tibia"J.Histochem.Cytochem.. 50(7). 945-953 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakamura, H. et al.: "Immunolocalization of keratan sulfate proteoglycan in rat calvaria"Arch.Histol.Cytol.. 64(1). 111-120 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takeyama, M. et al.: "Immunohistochemical demonstration of cathepsin B and L in periodontal ligament (PDL) of the rat molar"Jpn J.Oral Biol.. 43(2). 194-202 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Khairul Matin et al.: "Recombinant human bone morphogenetic protein-2 impact on residual ridge resorption after tooth extraction. An experimental study in rat"Int.J.Oral Maxillofac.Implants. 16(3). 400-411 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamamoto, T. et al.: "Ultracytochemical study of medullary bone calcification in estrogen injected male Japanese quail"Anat.Rec.. 264(1). 25-31 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ueno, et al.: "Cellular origin of endochondral ossification from grafted periosteum"Anat.Rec.. 264. 348-357 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakamura, H. et al: "Localization of osteoprotegerin (OPG) on bone surfaces and cement lines in rat tibia"J.Histochem.Cytochem.. 50(7). 945-953 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakamura, H.et al.: "Localization of osteoprotegerin (OPG) on bone surface and cement lines in rat tibia"J.Histochem.Cytochem.. 50(7). 945-953 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakamura, H.et al.: "The role of Osteoclast extracellular signal-regulated kinase (ERK) in cell survival and maintenance or cell-polarity"J.Bone Miner.Res.. 17(in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 中村浩彰, 山本敏男: "骨芽細胞と破骨細胞の相互作用"CLINICAL CALCIUM. 13(in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 中村浩彰 他: "破骨細胞におけるMAPkinase系ERKに関する形態学的解析"Acta Anatomica Nipponica. 77(1). 34 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 中村浩彰 他: "破骨細胞のExtracellular signal regulated kinase (ERK)に関する形態学的解析"日本骨代謝学会 プログラム抄録集. 101 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 辻 岳人, 中村浩彰 他: "四肢短縮小人症Ellis-van Cleveled症候群の遺伝子であるEVC遺伝子の軟骨細胞における発現"日本骨代謝学会 プログラム抄録集. 112 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 香川智正, 中村浩彰 他: "ラット頭蓋骨骨延長モデルの確立と骨形成過程の組織学的研究"日本骨代謝学会 プログラム抄録集. 154 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 中村浩彰 他: "エナメル芽細胞におけるExtracellular signal-regulated kinase (ERK)に関する形態学的研究"歯基礎誌. 44(5). 70 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 仲田直樹, 中村浩彰 他: "骨芽細胞と破骨細胞の相互作用"歯基礎誌. 44(5). 89 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 中村浩彰: "破骨細胞におけるシグナル伝達系"Jap.J. Pharm. 85. 469 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakamura, H.: "Immunolocalization of keratan sulfate proteoglycan in rat calvaria"Arch.Histol.Cytol. 64(1). 111-120 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamamoto, T. et al.: "Ultracytochemical study of medullary bone calcification in estrogen injected male Japanese quail"Anaf.Rec. 264(1). 25-31 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 中村浩彰: "骨組織におけるオステオプロテジェリン局在"Acta Anatomica Nipponica. 76(1). 137 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 中村浩彰: "MAP kinase系ERKは破骨細胞の波状縁形成を制御する"日骨代謝誌. 19(2). 77 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 中村浩彰: "吸収骨表面,セメントラインにおけるオステオプロテジェリン局在"歯基礎誌. 43(5). 131 (2001)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi