| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2002: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Research Abstract |
We found that when present in the culture medium, chick serum (CKS) modulated the phenotypic change from chondrocytes of Meckel's cartilage to adipocytes in vitro as revealed by light and electron microscopy, the incorporation of BrdU and immunocytochemistry. CKS inhibited DNA synthesis in chondrocytes and the proliferation of these cells, while it facilitated the differentiation to adipocytes. Immunocytochemical staining of adipocytes with stage-specific antibodies revealed the presence of immunoreactive uncoupling protein (UGP-1) and peroxisome proliferator-activated receptor (PPAR γ ) in immature adipocytes, and leptin and glucose transporter (Glut-4) in mature adipocytes. The adipocytes that were formed in the present study were multilocular adipocytes that contained many small lipid droplets but, in many ways, they resembled white adipocytes. However, little is known about the effects on the other kinds of mesenchymal cells. Therefore, we further examined adipogenesis in the osteogenic MC 3T3 -E1 cells, subcutaneous fibroblasts, and undifferentiated dental pulp cells. It was suggested that CKS agents involved in adipocyte differentiation are a common inductive factor affecting various mesenchymal cells.
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