Mechanisms of cytotoxicity by initiators for dental polymerizing resin systems and application for photodiamic therapy.
| Project/Area Number |
13671948
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Meikai University |
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Principal Investigator |
ATSUMI Toshiko Meikai U., Sch. Dent., associate professor, 歯学部, 助教授 (60049385)
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| Co-Investigator(Kenkyū-buntansha) |
FUJISAWA Seiichiro Meikai U., Sch. Dent., Professor, 歯学部, 教授 (40014162)
SUGITA Kenji Meikai U., Sch. Dent., Lecturer, 歯学部, 講師 (90171157)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | camphorquinone / 9-fluorenone / photosensitizer / free radical / cytotoxicity / human pulp fibroblasts / reactive oxygen species / liposomes / クルクミン / DPPH / 細胞障害性 / アポトーシス |
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| Research Abstract |
Camphorquinone (CQ) is widely used as a photo-initiator in dental materials ; however, its cytotoxicity against human pulp fibroblasts (HPF) and particularly the effects of 2-dimethylaminoethyl methacrylate (DMA), a reducing agent, and visible-light (VL) irradiation on it remains unknown. So we investigated the cytotoxic and reactive oxygen species (ROS)-producing effects of CQ with or without DMA, in the presence or absence of VL on HPF cells. Also, the free-radical production activity of CQ was measured by 2 different methods (using diphenylpicryl hydrazyl, DPPH, and galvinoxyl). Furthermore, the phase-transition properties of dipalmitoylphosphatidyl choline (DPPC) liposomes, as a model for biomembranes, induced by CQ were investigated by differential scanning calorimetry (DSC). These findings were compared with those of 9-fluorenone (9F), an aromatic photo-initiator with long conjugated groups. CQ with VL irradiation increased the radical production, whereas 9F with VL irradiation increased ROS production, as well as changes in the DPPC phase-transition properties. The cytotoxicity of CQ toward HPF cells was smaller than that of 9F despite greater radical production. The addition of DMA to photosensitizer enhanced the free-radical production without in creasing ROS and cytotoxicity. In conclusion, CQ/DMA is valuable for polymerization of dental resins.
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Report
(4 results)
Research Products
(9 results)
