| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2001: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Research Abstract |
We examined the effects of FOS consumption on gastrectomy-evoked osteopenia and disorders of dentin formation in rats. Twenty-eight 35-d-old male Sprague Dawley rats were equally divided into two groups; sham-operated (SH) and gastrectomized (GX), and sham-operation or total gastrectomy was performed. Four weeks after each surgery, the rats were divided into two sub-groups ; with or without 7.5% fructooligosaccharide (FOS)-feeding (SH, SH+FOS, GX and GX+FOS) for 6 weeks. Backscattered electron images of the mandibular sections were taken to calculate trabecular bone area (TBA), cortical bone area (CBA) and total scan area (TA). Thereafter, the dentin apposition rate in maxilla, and the enamel, dentin and dental pulp area in mandibula were calculated using a fluorescent microscope. TBA and CBA in GX rats were markedly decreased. FOS-feeding significantly counteracted this reduction, but not to the level seen in SH rats, TA in GX groups was significantly decreased. The dentin and enamel areas were significantly reduced in GX rats, and the dental pulp area in GX+FOS rats was significantly reduced.The rate of dentin formation was identical among the groups,except the GX group. These results suggest that FOS consumption partially restored osteopenia and completely improved the reduction of dentin formation following gastrectomy.
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