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Risk assessment of peri-inplantitis patients with chair-side MMP-8 assay system

Research Project

Project/Area Number 13672031
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 補綴理工系歯学
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

ARAKAWA Hikaru  Okayama University Graduate School of Medicine and Dentistry, Research Associate, 大学院・医歯学総合研究科, 助手 (30304314)

Co-Investigator(Kenkyū-buntansha) KASAI Teruo  Okayama University, Dental Hospital, Research Associate, 歯学部附属病院, 助手 (00335613)
YATANI Hirofumi  Okayama University Graduate School of Medicine and Dentistry, Professor, 大学院・医歯学総合研究科, 教授 (80174530)
KUBOKI Takuo  Okayama University Graduate School of Medicine and Dentistry, Assistant Professor, 大学院・医歯学総合研究科, 助教授 (00225195)
完山 学  岡山大学, 歯学部・附属病院, 講師 (90294420)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2001: ¥3,100,000 (Direct Cost: ¥3,100,000)
KeywordsDental Implants / Peri-implantitis / Matrix metalloproteinases / Gingival crevicular fluids / bone-resorption / Western blotting / Risk marker / MMPs / ELISA法 / PCR法 / コラゲナーゼ
Research Abstract

Matrix metalloproteinases (MMPs) are known to play an important role in pathological tissue destruction in the periodontal tissues. Therefore, MMPs related to bone matrix degradation could be a possible marker for ongoing bone destruction around the peri-implantitis implants. In this study, four peri-implantitis patients (male/female : 2/2 ; mean age : 68.5^^+__-6.7 yrs ; 9 implants ) and age- and sex- matched four control patients (male/female : 2/2, mean age : 66.0^^+__-5.8 yrs, 8 implants) were selected from 64 consecutive patients who were installed implant-supported superstructures in our school from 1990 to 2000. Inclusion criteria for selection of the peri-implantitis and control patients in this study were having an implant "with accumulated vertical bone loss (ABL) of more than 0.6 mm per year" and "with ABL of less than 0.2 mm per year" after superstructure installation, respectively. Bone levels around implants were assessed by dental x-ray films taken annually and mean valu … More es of three calibrated observers readings were used as outcome. Ongoing vertical bone loss per year (OBL) after superstructure installation was also calculated. Gingival crevicular fluids (GCFs) were collected at the mesio-buccal comer of the gingival crevices by using absorbent paper points. Then the samples were dissolved in 0.1% phoshate buffered saline with Tween 20 (PBS-T) and stored at -20℃. MMPs in GCFs were detected by Western blotting using anti-human monoclonal antibodies specific for MMP-1, -8 and -13 (Fuji Chemical Industry, Japan) with positive controls. As the results, in the peri-implantitis patients, MMP-8 was detected in three actively bone-resorbing implants whose OBL at GCF sampling was more than 0.8 mm and not detected in the other six implants whose OBL at GCF sampling was less than 0.3 mm, while no MMP-8 was detected in the controls. MMP-1 and -13 could not be detected in any GCFs in this study. These results suggest that MMP-8 might be a marker for prediction of ongoing bone destruction around the peri-implantitis implants. Less

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] 荒川 光 ほか: "口腔インプラントの生存に関する疫学調査:オッセオインテグレーションの獲得と維持からみた評価"日本口腔インブラント学会誌. 15・1. 67-74 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 荒川 光: "高度の歯槽骨吸収を伴った再植歯を抜歯後に口腔インプラントで治療を行った一症例"日本補綴歯科学会雑誌. 46・5. 758-759 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 荒川 光 ほか: "口腔インプラント患者の分子生物学的マーカによるリスク評価-口腔インプラント周囲歯肉溝滲出液中のMMPsの検出-"日本口腔インプラント学会誌. 14・1. 99 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Arakawa et al.: "Matrix Metalloproteinases in Gingival Crevicular Fluids of Peri-implantitis Patients"Journal of Dental Research 80(Special Issue). 212 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Arakawa et al: "A clinical follow-up study on acquisition and maintenance of osseointeguration in relation to the risk factors for osseodisintegration"J.Jpn.Soc.Oral Implant. 15, (in Japanese). 67-74 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Arakawa: "Oral implant treatment after extracing replanted teeth with severe root and bone resorption : A case report"J Jpn Prosthodont Soc. 46, (in Japanese). 758-759 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Arakawa et al: "Risk assessment of peri-inplantitis patients with Matrix metalloproteiases in gingival crevicular fluids"J.Jpn.Soc.Oral Implant. 14, (in Japanese). 99 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Arakawa et al: "Matrix Metalloproteinases in Gingival Crevicular Fluids of Peri-implantitis Patients"J.Dent.Res.. 80. 212 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 荒川 光 ほか: "口腔インプラントの生存に関する疫学調査:オッセオインテグレーションの獲得と維持からみた評価"日本口腔インプラント学会誌. 15・1. 67-74 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 荒川 光ほか: "オッセオインテグレーションの獲得と維持からみた口腔インプラントの臨床予後評価"日本補綴歯科学会雑誌. 46特別号. 58 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 荒川 光 ほか: "インプラント周囲炎リスクマーカーとしてのMMP-8の可能性"日本IMZ・フリアリット-2インプラント研究会 第7回学術大会・症例検討会抄録集. 7. 15 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Arakawa et al.: "Matrix Metalloproteinases in Gingival Crevicular Fluids of Peri-implantitis Patient"Journal of Dental Research 80. (Special Issue). 212 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 荒川 光 ほか: "口腔インプラントの生存に関する疫学調査:オッセオインテグレーションの獲得と維持からみた評価"日本口腔インプラント学会誌. 15・1(印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 荒川 光 ほか: "インプラント周囲炎リスクマーカーとしてのMMP-8の可能性"日本IMZ・フリアリット-2インプラント研究会 第7回学術大会・症例検討会抄録集. 15 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 荒川 光: "高度の歯槽骨扱収を伴った再植歯を抜歯後に口腔インプラント治療を行った一症例"日本補綴歯科学会雑誌. 45特別号. 214 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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