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Development of functionally graded DNA films containing cytokines eliciting bone formation

Research Project

Project/Area Number 13672070
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 補綴理工系歯学
Research InstitutionFukuoka Dental College

Principal Investigator

FUKUSHIMA Tadao  Fukuoka Dental College, Dental Materials, Associate Professor, 歯学部, 助教授 (80084250)

Co-Investigator(Kenkyū-buntansha) HAYAKAWA Toru  Nihon University, School of Dentistry at Matsudo, Dental Materials, Assistant Professor, 松戸歯学部, 講師 (40172994)
OKAHATA Yoshio  Tokyo Institute of Technology, Biomolecular Engineering, Professor, 生命理工学部, 教授 (80038017)
INOUE Yusuke  Fukuoka Dental College, Dental Hygiene, Associate Professor, 歯科衛生学科, 助教授 (20105688)
KAWAI Tatsushi  Aichi-Gakuin University, School of Dentistry, Dental Materials, Professor, 歯学部, 教授 (60167351)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,700,000 (Direct Cost: ¥2,700,000)
KeywordsDNA / Lipid / Cytokine / DNA film / DNA-lipid complex / Antibacterial activity / Intercalation / Tensile strength
Research Abstract

The DNA-lipid complexes with and without cytokines BMP and b-FGF were prepared synthetic lipids from the reaction of n-alkyl alcohol (C=4〜C=20) and amino acids (glycine, L-alanine and L-glutamic acids) and the DNA films were obtained by casting the DNA -lipid complexes from chloroform/ethanol solution. The tensile strengths and intercalation behavior of DNA films were investigated. Antibacterials characteristics of DNA-lipid complexes against bacteria (P. gingivalis, S. mutans, S. aureus and P. aeruginosa) were also investigated. Additionally, effect of the DNA -lipid complexes with cytokines BMP and b-FGF on bone formation was evaluated. The DNA-lipid films had tensile strengths of approximately 2MPa, and formed intercalation complexes with ethidium bromide. The formation of the intercalation complex of DNA-lipid film with ethidium bromide was influenced by the length of the alkyl chains of the synthesized lipids. The complexes prepared from DNA with glycine decyl ester or glycine dodecyl ester, and with L-alanine decyl ester or glycine dodecyl ester showed antibacterial activity. However, the DNA-lipid complexes with cytokines BMP and b-FGF did not elicit bone formation.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Y.Inoue: "Antibacterial Characteristics of Newly Developed Amphiphilic Lipids and DNA-lipid, Complexes against Bacteria"Journal of Biomedical Materials Research. 65. 203-208 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y. Inoue, T. Fukushima, T. Hayakawa, H. Takeuchi, M. Kaminishi, K. Miyazaki, Y. Okahata: "Antibacterials characteristics of newly developed amphiphilic lipids and DNA-lipid complexes against bacteria"J Biomed Mater Res. 65A. 203-208 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Inoue, T.Fukushima, T.Hayakawa, H.Takeuchi, H.Kaminishi, K.Miyazaki, Y.Okahata: "Antibacterial Characteristics of Newly Developed Amphiphilic Lipids and DNA-lipid Complexes against Bacteria"Journal of Biomedical Materials Research. 65. 203-208 (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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