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Molecular biological analysis of oral multiple tumor and multiple precancer

Research Project

Project/Area Number 13672072
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

SATOH Akira  Hokkaido Univ., Grad. School of Dental Medicine, 大学院・歯学研究科, 助手 (90271684)

Co-Investigator(Kenkyū-buntansha) CHIBA Itsuo  Health Sciences Univ. Hokkaido School of Dentistry, 歯学部, 教授 (50250460)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsmultiple primary tumors / oral cancer / precancer / loss of heterozygosity / microsatellite alterations / 二次癌 / 口腔扁平上皮癌 / 前癌病変 / field cancerization theory
Research Abstract

Multiple primary tumors in patients with head and neck squamous cell carcinoma has become an increasingly important factor in clinical treatment decision. Currently, clinical and histologic parameters are used to determine whether or not multiple primary tumor is present Recent studies suggest that many multiple primary tumors in the upper aerodigestive tract have a common clonal origin, challenging the longstanding multiclonal origin concept. To determine genetic relationships among multiple oral cancerous and precancerous lesions, we analysed 100 lesions from 26 Japanese patients. Lesion development was synchronous and metachronous. We looked for patterns of microsatellite alterations using seven markers at chromosomes 3pl4, 9p21, and 17pl3, where microsatellite alteration occurs early in oral carcinogenesis. Loss of heterozygosity was found in 52.6%(41/78), 62.53% (60/96), and 59.3% (32/54) of informative multiple oral cancerous and precancerous lesions at 3p14, 9p21, and 17p13, respectively. Microsatellite instability was observed in 11, 26 and 13% of the samples at 3p14, 9p21, and 17pl3 markers, respectively. Patterns of microsatellite alterations were concordant in only nine (14%) of 63 lesions from four (18%) of 22 patients who initially presented with noninvasive lesions. However, two of four patients with invasive cancer as indexed lesion showed 16 (43%) clonally related multiple oral cancerous and precancerous lesions among 37 lesions (P=0.003). The results suggest that the majority of multiple oral cancerous and precancerous lesions arise from clonally independent cells affected by field cancerization. However, the probability of mucosal spread of clonal malignant or premalignant cells may increase along with malignant progression.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Jang S.J.et al.: "Multiple oral squamous epithelial lesions : are they genetically related?"Oncogene. 20. 2235-2242 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamazaki, Y. et al.: "Characteristics of p53 mutational spectrum in oral squamous cell carcinoma"Japanese Journal of Oral and Maxillofacial. 47. 389-399 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamazaki, Y: "Specific regions(L2,L3,LSH)of p53 mutations as poor prognostic factors in oral squamous cell carcinoma"Japanese Journal of Oral and Maxillofacial. 47. 649-655 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Chiba I: "Prevention of Betel Quid Chewer's Oral Cancer in the Asian-Pacific Area"Asian Pacific Journal of Cancer Prevention. 2. 263-269 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Topcu Z et al.: "CYP2A6 gene deletion reduces oral cancer risk in betel quid chewers in Sri Lanka"Carcinogenesis. 23. 595-598 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Jang, S. J. et al.: "Multiple oral squamous epithelial lesions : Are they genetically related?"Oncogene. 20. 2235-2242 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamazaki, Y. et al.: "Characteristics of p53 mutational spectrum in oral squamous cell carcinoma"Japanese Journal of Oral and Maxilofacial Surgery. 47. 389-399 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamazaki, Y.: "Specific regions (L2, L3, LSH) of p53 mutations as poor prognostic factors in oral squamous cell carcinoma"Japanese Journal of Oral and Maxilofacial Surgery. 47. 643-655 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Chiba, I.: "Prevention of betel quid chewers' oral cancer in the Asian-Pacific area"Asian Pacific Journal of Cancer Prevention. 2. 263-269 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Topcu, Z., et al.: "CYP2A6 gene deletion reduces oral cancer risk in betel quid chewers in Sri Lanka"Caccinogenesis. 23. 595-598 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Jang, SJ, et al.: "Multiple oral squamous epithelial lesions : Are they genetically related?"Oncogene. 20. 2235-2242 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Topcu, Z, et al.: "CYP2A6 gene deletion reduces oral cancer risk in betel quid chewers in Sri Lanka"Carcinogenesis. 23(4). 595-598 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yamazaki, Y, et al.: "Specific p53 mutations predict poor prognosis in oral squamous cell carcinoma"Oral Oncogene. 39(2). 163-169 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Chiba, I.: "Prevention of betel quid chewers' oral cancer in the Asian-Pacific Area"Asian Pacific Journal of Cancer Prevention. 2. 263-269 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Jang, SJ., et al.: "Multiple oral squamous epithelial les! ons! Are they genetially related?"Oncogene. 20. 2235-2242 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Topcu, Z., et al.: "CYP2A6 gene deletion reduces oral cancer risk"Carcinogenesis. (in press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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