| Project/Area Number |
13672078
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE (2002)
Tohoku University (2001) |
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Principal Investigator |
FUMINORI Katou HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE, School of Medicine, Associate Professor (60204492)
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| Co-Investigator(Kenkyū-buntansha) |
大谷 明夫 東北大学, 大学院・医学系研究科, 助教授 (30133987)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Oral / Transplanted skin / Chemokine / Dendritic cell |
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| Research Abstract |
Macrophage-derived chemokine (MDC/CCL22) is chemokine ligand for its specific receptor CC chemokine receptor 4 (CCR4) and another chemokine ligand, CCL19 (EBI1 ligand chemokine ; ELC), is the shared receptor for CCR7. We investigated the expression of wo chemokin systems, CCL22/CCR4 and CCL19/CCR7 in the intraorally transplanted skin and lymph node to know the dynamics in the defense system of the intraorally transplanted skin.
Reverse transcriptase-polymerase chain reaction analysis revealed mRNA expression for MDC and CCR4 in the inflamed skin and neck lymph nodes (LNs), but not in normal skin. Meanwhile, RT-PCR analysis revealed the expression of CCL19 and CCR7 in LNs, but not in the inflamed skin. Immunohistochemically, MDC+ cells and CCR4+ cells were located both in the dermis of inflamed skin and the T cell area of LNs. MDC+ cells were identified to be DCs both in inflamed skin and LNs. The majority of CCR4+ cells were CD4+ T cells, accounting for approximately one-third of total CD4+ T cells in the inflamed skin. The majority of DC-Lamp(+) mature DCs in the T-cell area of LNs expressed CCL19 and were surrounded by CCR7(+) lymphocytes. In contast, the majority of DC-Lamp(+) mature DCs in inflamed skin were totally negative for CCL19 and were surrounded by CCR7(-) T cells.
The present study revealed a difference in the function of mature DCs between LNs and chronically inflamed skin.
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