• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Application of 5-aminorevulinic acid for diagnosis and treatment of oral cancer

Research Project

Project/Area Number 13672080
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionUniversity of Tsukuba

Principal Investigator

ONIZAWA Kojiro  University of Tsukuba, Institute of Clinical Medicine, Assistant Professor, 臨床医学系, 講師 (60194578)

Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsFluorescence / Oral Cancer / Aminorevulinic acid / Photodynamic Therapy / Autofluorescence
Research Abstract

This study was carried out to investigate application of 5-anunorevulinic acid (ALA) for diagnosis and treatment of oral cancer. The relationship between autofluorescence and AL.A induced fluorescence was analyzed biochemically, and effect of photodynamic therapy using AIA on nude mouse tumor was evaluated histologically. The autofluorescence arising in human oral cancer was reported to be associated with protoporphyrin, and similar poiphyrins were observed in oral cancers induced in hamsters and oral-cancer cell lines. After topical or systemic administration of ALA, red fluorescence which was similar to autofluorescence in human cancer was observed in oral mucosa and cancer induced in hamster. Red fluorescence was also observed in slap and tumor formed by human oral-cancer cell lines under administration of AIA. The fluorescent substance associated with ALA was suggested to be protoporphyrin, which was consistent with those caused autofluorescence in human cancer. The enhanced intensity of red fluorescence was also demonstrated in cancer tissues of hamster or nude mouse under administration of ALA, and ALA could be applied to fluorescence diagnosis for oral cancer to enhance intensity of autofluorescence in early-staged or small-sized cancer. Although ALA-based photodynamic therapy was evaluated in nude mouse honor under various experimental conditions, tumor necrosis was observed in only superficial area and nodular tumor did not show any regression after the therapy. Further experiments would be necessary before clinical application of ALA-based photodynamic therapy for nodular cancer tissue

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Onizawa K., et al.: "Analysis of fluorescence in oral squamous cell carcinoma"Oral Oncology. 38. 343-348 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Onizawa K., et al.: "Characterization of autofluorescence in oral squamous cell carcinoma"Oral Oncology. 39. 150-156 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Onizawa K.et al.: "Analysis of fluorescence in oral squamous cell carcinoma"Oral Oncology. 38. 343-348 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Onizawa K.et al.: "Characterization of autofluorescence in oral squamous cell carcinoma"Oral Oncology. 39. 150-156 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Onizawa, K., et al.: "Characterization of autofluorescence in oral squamous cell carcinoma"Oral Oncology. 39・2. 150-156 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Onizawa K., et al.: "Analysis of fluorescence in oral squamous cell carcinoma"Oral Oncology. 38・4. 343-348 (2002)

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi