| Project/Area Number |
13672092
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Shimane Medical University |
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Principal Investigator |
NARIAI Yoshiki Shimane University, Faculty of Medicine, Assistant Professor, 医学部, 助手 (60333465)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUDA Hajime Shimane University, Faculty of Medicine, Senior Resident, 医学部, 医員
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2001: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Oral mucous membrane diseases / Oral lichen planus / Superoxide anion / Biscoclaurine alkaloids / Cytokine / Antioxidant enzyme / スーパーオキサイド |
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| Research Abstract |
The purpose of this study was to investigate the pathogenesis of various oral membrane diseases, in particular oral lichen planus, by examining the relationships between effects of biscoclaurine alkariods (BA; cepharanthin^<(R)>) on clinical symptoms and imunohistichemical stainings of cytokines or antioxidant enzymes. 30 tissue samples were obtained by biopsy from the patients with oral lichen planus. Immunostaining was performed using the streptavidin-biotin method. Correlation between Clinical improvement by treatment of biscoclaurine alkaloids and expressions of cytokines (TNF-α, IFN-γ, IL-1β) or antioxidant enzymes (manganese-containing superoxide dismutase : Mn-SOD, glutathione peroxidase : GPX) were evaluated using x^2-test and logistic regression. The results were as follow.
1. The expressions of TNF-α, IFN-γ and IL-1β were detected in 17/30 (56.7%), 221/30 (73.3%) and 13/30 (43.3%) of the cases.
2. TNF-α expression had significant relationship between IFN-γ or IL-1β expression.
3. The negativity of TNF a was statistically correlated with effects of BA (P=0.046).
4. The expressions of Mn-SOD and GPX were detected in 16/30 (53.3%) and 17/30 (56.7%) of the cases.
5. The expression of Mn-SOD was a significant factor for the effects of BA on clinical symptoms.
These results suggest that some kinds of cytokines and antioxidant enzymes may be related to pathogenesis of OLP and action of BA on OLP.
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