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Basic research of gene therapy combination with radio, chemo and hyperthermic treatment on oral carcinoma

Research Project

Project/Area Number 13672110
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionNara Medical University

Principal Investigator

KIRITA Tadashi  Nara Medical University, Oral and Maxillofacial Surgery, Professor, 医学部, 教授 (70201465)

Co-Investigator(Kenkyū-buntansha) IMAI Yuichiro  Nara Medical University, Oral and Maxillofacial Surgery, Research Associate, 医学部, 助手 (80347567)
OHNISHI Takeo  Nara Medical University, Biology, Professor, 医学部, 教授 (60094554)
杉村 正仁  奈良県立医科大学, 医学部, 教授 (20028749)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2001: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsChemical Chaperone / Glycerol / Radiotherapy / Hyperthermic therapy / Chemotherapy / Apoptosis / p53
Research Abstract

Objects: We designed to elevate therapeutic efficacy of radiation by addition of glycerol on an oral squamous cell carcinoma cell line (Ca9-22) bearing mutation in a tumor suppressor gene, p53 (mp53).
Methods: To examine the effectiveness of glycerol in radiation-induced lethality for Ca9-22 cells, we performed colony formation assay. Apoptosis was analyzed with Hoechst 33342 staining, Western blotting and DNA ladder formation assay. Ca9-22 cells became radiosensitive when glycerol was added to culture medium before X-ray irradiation.
Results: In accordance with the cell survived rate, apoptosis (apoptotic body and DNA fragmentation) was induced by X-rays irradiation after glycerol treatment while there was little apoptosis induced by X-ray irradiation or glycerol addition alone. Bax protein was accumulated after combined treatment of X-ray irradiation plus glycerol, but not after X-ray irradiation.
Conclusions: These findings suggested that pre-treatment with glycerol might be effective in therapy against mp53 cancer cells.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (57 results)

All Other

All Publications (57 results)

  • [Publications] Yasumoto, J., et al.: "Analysis of apoptosis-related gene expression after-X-ray irradiation in Human tongue squamous cell carcinoma cells harboring wild-type or mutation-type p53 gene"J.Rad.Res.. (In press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohnishi, K., et al.: "P53-dependent hyperthermic enhancement of tumour inhibition by Xray or Carbon-ion beam irradiation"Int.J.Hyperthermia.Mol.Cancer. (In press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masunaga S., et al.: "Usefulness of combined treatment with mild hyperthremia and/or tirapazamine in the treatment of solid tumors : its independence of p53 status"Cancer Science. (In press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Asakawa, I., et al.: "Growth inhibitionin transplanted human tongue tumors bearing different p53 gene status with X-ray or C-beam irradiation"Anticancer Res.. 22. 2037-2043 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohnishi, K., et al.: "Transfection of mutant p53 gene depresses X-ray-or CDDP-induced apoptosis in a human squamous cell carcinoma of the head and neck"Apoptosis. 7. 367-372 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masunaga, S., et al.: "Impact of p53 status of the tumor cells on the effect of reactor neutron beam irradiation with emphasis on the response of intratumor quiescent cells"Jpn.J.Cancer Res.. 93. 1366-1377 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takahashi, A., et al.: "Radiation-induced apoptosis in scid mouse spleen after low dose-rate irradiation"Int.J.Radiat.Biol.. 78. 689-693 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Harima, Y., et al.: "Human papilloma virus (HPV) DNA associated with prognosis of cervical cancer after radiotherapy"Int.J.Radiat.Oncol.Biol.Phys.. 52. 1345-1351 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Harima, Y., et al.: "Mutation of the PTEN gene in advanced cervical cancer correlated with tumor progression and poor outcome after radiotherapy"Int.J.Oncol.. 18. 493-497 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takahashi, A., et al.: "p53-dependent thermal enhancement of cellular sensitivity in human squamous cell carcinomas in relation to LET"Int. J. Radiat. Biol.. 77. 1043-1051 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takahashi, A., et al.: "Radiation response of apoptosis in C57BL/6N mouse spleen after whole-body irradiation"Int.J.Radiat.Biol.. 77. 939-946 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Harima, Y., et al.: "Chromosome 6p21.2, 18q21.2 and human papilloma virus (HPV) DNA can predict prognosis of cervical cancer after radiotherapy"Int.J.Cancer. 96. 286-296 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohnishi, T., et al.: "Tumor suppressor p53 response is blunted by pre-irradiation at a low-dose-rate radiation"Physica Medica (Eur. J. Med. Physics). 17 Suppl.1. 215-216 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Harima, Y., et al.: "Polymorphism of the WAF1 gene is related to susceptibility cervical cancer in Japanese women"Int.J.Mol.Medicine. 7. 261-264 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohnishi, K., T.Ohnishi: "Heat-induced p53-dependent signal transduction and its role in hyperthermic cancer therapy"Int.J.Hyperthermia. 17. 415-427 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsumoto, H., et al.: "Induction of radioresistance by a nitric oxide-mediated bystander effect"Radiat.Res.. 155. 387-396 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kirita, T., et al.: "A new strategy for cancer therapy based on a predictive indicator"Human Cell. 14. 1-6 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 大西健他: "集学的癌治療の研究と臨床"篠原出版新社. 7 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 太田一郎他: "癌放射線療法2002"篠原出版新社. 12 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 桐田忠昭他: "脳の科学"星和書店. 7 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yasumoto, J., et al.: "Analysis of apoptosis-related gene expression after X-ray irradiation in Human tongue squamous cell carcinoma cell harboring wild-type or mutation-type p53 gene"J. Rad. Res.. in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohnishi, K., et al: "P53 -dependent hyperthermic enhancement of tumor inhibition by Xray or Carbon-ion beam irradiation"Int. J. Hyperthermia. Mol. Cancer. in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masunaga S., et al: "Usefulness of combined treatment with mild hyperthermia and/or tirapazamine in the treatment of solid tumors: its independence of p53 status"Cancer Science. in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Asakawa, I., et al.: "Growth inhibition in transplanted human tongue tumors bearing different p53 gene status with X-ray or C-beam irradiation"Anticancer Res.. 22. 2037-2043 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohnishi, K., et al.: "Transfection of mutant p53 gene depresses X-ray- or CDDP-induced apoptosis in a human squamous cell carcinoma of the head and neck"Apoptosis. 7. 367-372 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masunaga, S., et al.: "Impact of p53 status of the tumor cells on the effect of reactor neutron beam irradiation with emphasis on the response of intratumor quiescent cells"Jpn. J. Cancer Res.. 93. 1366-1377 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takahashi, A., et al.: "Radiation-induced apoptosis in scid mouse spleen after low dose-rate irradiation"Int. J. Radiat. Biol.. 78. 689-693 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Harima, Y., et al.: "Human papilloma virus (HPV) DMA associated with prognosis of cervical cancer after radiotherapy"Int. J. Radiat. Oncol. Biol. Phys.. 52. 1345-1351 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Harima, Y., et al.: "Mutation of the PTEN gene in advanced cervical cancer correlated with tumor progression and poor outcome after radiotherapy"Int. J. Oncol.. 18. 493-497 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takahashi, A., et al.: "p53-dependent thermal enhancement of cellular sensitivity in human squamous cell Carcinomas in relation to LET"Int. J. Radiat. Biol.. 77. 1043-1051 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takahashi, A., et al.: "Radiation response of apoptosis in C57BL/6N mouse spleen after whole- body irradiation"Int. J. Radiat. Biol.. 77. 1043-1051 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Harima, Y., et al.: "Chromosome 6p21.2. 18q21.2 and human papilloma virus (HPV) DNA can predict prognosis of cervical cancer after radiotherapy"Int. J. Cancer. 96. 286-296 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohnishi, T., et al.: "Tumor suppressor p53 response is blunted by pre-irradiation at a low-does-rate radiation"Physica Medica (Eur. J. Med Physics). 17 Suppl., 1. 215-216 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Harima, Y., et al.: "Polymorphism of the WAF gene is related so susceptibility to cervical cancer in Japan women"Int. J. Mol. Medicine. 7. 261-264 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohnishi, K & T, Ohnishi: "Heat-induced p53-dependent signal transduction and its role in hypertheramis cancer therapy"Int. J. Hyperthemia. 17. 415-427 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsumoto, H., et al: "Induction of radioresistance by a nitric oxide-mediated bystander effect"Radiat. Res.. 155. 387-396 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kirita, T., et al.: "A new strategy for cancer therapy based on a predictive indicator"Human Cell. 14. 1-6 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yasumoto, J., et al.: "Analysis of apoptosis-related gene expression after X-ray irradiation in Human tongue squamous cell carcinoma cells harboring wild-type or mutation-type p53 gene"J. Rad. Res.. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ohnishi, K., et al.: "P53-dependent hyperthermic enhancement of tumour inhibition by Xray or Carbon-ion beam irradiation"Int. J. Hyperthermia. Mol. Cancer.. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Masunaga, S,. et al.: "Usefulness of combined treatment with mild hyperthermia and/or tirapazamine in the treatment of solid -its independence of p53 status"Cancer Science,. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Asakawa, I., et al.: "Growth inhibition in transplanted human tongue tumors bearing different p53 gene status with X-ray or C-beam irradiation"Anticancer Res.. 22. 2037-2043 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ohnishi, K., et al.: "Transfection of mutant p53 gene depresses X-ray-or CDDP-induced apoptosis in a human sauamous cell carcinoma of the head and neck"Apoptosis. 7. 367-372 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Masunaga, S., et al.: "Impact of p53 status of the tumor cells on the effect of reactor neutron beam irradiation with emphasis on the response of intratumor quiescent cells"Jpn. J. Cancer Res.. 93. 1366-1377 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takahashi, A., et al.: "Radiation-induced apoptosis in scid mouse spleen after low dose-rate irradiation"Int. J. Radiat. Biol.. 78. 689-693 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Harima, Y., et al.: "Human papilloma virus (HPV) DNA associated with prognosis of cervical cancer after radiotherapy"Int. J. Radiat. Oncol. Biol. Phys.. 52. 1345-1351 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Harima, Y., et al.: "Mutation of the PTEN gene in advanced cervical cancer correlated with tumor progression and poor outcome after radiotherapy"Int. J. Oncol.. 18. 493-497 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takahashi, A., et al.: "p53-dependent thermal enhancement of cellular sensitivity in human squamous cel carcinomas in relation to LET"Int. J. Radiat. Biol.. 77. 1043-1051 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takahashi, A., et al.: "Radiation response of apoptosis in C57BL/6N mouse spleen after whole-body irradiation"Int. J. Radiat. Biol.. 77. 939-946 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Harima, Y., et al.: "Chromosome 6p21.2, 18q21.2 and human papilloma virus (HPV) DNA can predict prognosis of cervical cancer after radiotherapy"Int. J. Cancer. 96. 286-296 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Olinishi, T., et al.: "Tumor suppressor p53 response is blunted by pre-irradiation at a low-dose-rate radiation"Physica Medica (Eur. J. Med. Physics). 17 Suppl.1. 215-216 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Harima, Y., et al.: "Polymorphism of the WAF1 gene is related to susceptibility to cervical cancer in Japanese women"Int. J. Mol. Medicine. 7. 261-264 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ohnishi, K., T.Ohnishi: "Heat-induced p53-dependent signal transduction and its role in hyperthermic cancer therapy"Int. J. Hyperthmnia.. 17. 415-427 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Matsumoto, H., et al.: "Induction of radioresistance by a nitric oxide-mediated bystander effect"Radiat. Res.. 155. 387-396 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kirita, T., et al.: "A new strategy for cancer therapy based on a predictive indicator"Human Cell. 14. 1-6 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 大西健 他: "集学的癌治療の研究と臨床"篠原出版新社. 7 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 太田一郎 他: "癌放射線療法2002"篠原出版新社. 12 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 桐田忠昭 他: "脳の科学"星和出版. 7 (2001)

    • Related Report
      2002 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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