| Project/Area Number |
13672114
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
MUTO Toshitaka Health Sciences University of Hokkaido, Sch. of Dentistry, Associate Professor, 歯学部, 助教授 (00143298)
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| Co-Investigator(Kenkyū-buntansha) |
KAKU Tohru Health Sciences University of Hokkaido, Sch. of Dentistry, Professor, 歯学部, 教授 (60133253)
YAJIMA Toshihiko Health Sciences University of Hokkaido, Sch. of Dentistry, Professor, 歯学部, 教授 (10018749)
KAWAKAMI Johji Health Sciences University of Hokkaido, Sch. of Dentistry, Assistant Professor, 歯学部, 助手 (20244858)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | Temporomandibular joint / Hypermobility / Synovitis / Rat / Early response / Light microscopy / Electronmicroscopy |
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| Research Abstract |
The experiments consist of two study.
Synovitis of the temporomandibular joints of 8-week-old male Wister rats was induced by forced condylar hypermobility, which was administered 1.0 times successively once a day for 10 days. Pathologic changes were observed microscopically at 2 and 5 days and 2, 4, 6, 8, 10 and 20 weeks after treatment. Histokogic findings from the 5th day to the 6th week after treatment were characterized by multiple layers of synovial cells, an increase of dilated vasculature, fibrin deposits, and synovial adhesion. These findings were in good agreement with those reported f or the human temporomandibutar joint. The pathologic changes decreased with advancing time, and synovitis was not observed at 20 weeks after treatment. The present procedure for including synovitis of the temporomandibular joint in rats will be useful in understanding the pathogenesis of synovitis in human temporomandibular joints.
Electron microscopy was used to examine the histologic effect of trauma on the rat temporomandibular joint synovial membrane. Trauma to the TMJ in male Wister rats (100-200g) was introduced through repeated forced condylar hypermobility. Ultrastructural observations were made 5 days and 6 weeks after the trauma. The early response of t he synovial membrane was synovial hyperplasia, type A synovial cell loss, dilation of the r-ER in the type B synovial cells and fibrin deposition on the synovial surfaces. The late, response included degeneration of synovial cells with swollen mitochondria and cell projections, and cell fragmentation. Large amount of fibrin deposition on opposing surface layers was also niticed. The type A cell loss and fibrin deposition followed by the occurrence of fibrinous materials at opposing surface layers of the synovial membrane suggest that traumatic synovitis causes synovial adhesions.
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