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Study on the role of small G proteins in mechanotransduction

Research Project

Project/Area Number 13672138
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 矯正・小児・社会系歯学
Research InstitutionTohoku University

Principal Investigator

IGARASHI Kaoru  Tohoku University, Graduate School of Dentistry, Professor, 大学院・歯学研究科, 教授 (70202851)

Co-Investigator(Kenkyū-buntansha) SAEKI Shuichi  Tohoku University, Graduate School of Dentistry, Instructor, 大学院・歯学研究科, 助手 (60271954)
WOO Je-tae  Chubu University, College of Bioscience and Biotechnology, Associated Professor, 応用生物学部, 助教授 (20272693)
三谷 英夫  東北大学, 大学院・歯学研究科, 教授 (50014220)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsSmall G Protein / Rho / Mechanical Stimulus / Bone / Bone Formation / Osteoblast / International Exchange of Researcher / United States of America
Research Abstract

Mechanical stimulus is an essential factor for the maintenance of bone homeostasis. It is important to clarify the signal transduction pathway of mechanical stimulation (mechanotransduction pathway) leading to bone formation. In the present study, we investigated the role of Rho, a small G protein that regulates cellular configuration, in the mechanotransduction using pharmacological approach.
1.Development of a micromechanical testing device and establishment of the assay system
We developed an improved loading device capable of delivering loads with milli-Newton resolution and micrometer displacements to viable bone explants and determined conditions of loading which give the maximum anabolic responses.
2.Pharmacological study using a tissue culture system and the micromechanical testing device
It was suggested that small G proteins of Rho family might act as a negative regulator in the mechanotransduction pathway that leads to bone formation.
3.Pharmacological study using a cell culture system and the Flexercell Strain Unit
It was suggested that small G proteins of Rho family are involved in the nitric oxide production by osteoblasts. However, it is not known whether they have any role in the signal transduction of a cyclic tensional stimulus in osteoblastic cells.
4.Future prospect
Although the present results suggest that small G proteins of Rho family, are involved in the mechanotransduction pathway in bone, their role remains unclear and roles of the other families of small G proteins are not known. Further studies using molecular biological approach as well as pharmacological approach would be required to solve these questions.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Kunnel JG, Igarashi K: "Bone anabolic responses to mechanical load in vitro involve COX-2 and constitutive NOS."Connective Tissue Research. 45. 1-10 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Singh ATK: "Regulation of parathyroid hormone-stimulated phospholipase D in UMR-106 cells by calcium, MAP kinase, and small G proteins."Journal of Bone and Mineral Research. 18. 1453-1460 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kunnel JG: "In vitro mechanical and cellular responses of neonatal mouse bones to loading using a novel micromechanical-testing device."Calcified Tissue International. 71. 499-507 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kunnel JG, Igarashi K: "Bone anabolic responses to mechanical load in vitro involve COX-2 and constitutive NOS"Connective Tissue Research. 45. 1-10 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Singh ATK: "regulation of parathyroid hormone-stimulated phospholipase D in UMR-106 cells by calcium, MAP kinase, and small G proteins"Journal of Bone and Mineral Research. 18. 1453-1460 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kunnel JG: "In vitro mechanical and cellular responses of neonatal mouse bones to loading using a novel micromechanical-testing device"Calcified Tissue International. 71. 499-507 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kunnel JG, Igarashi K: "Bone anabolic responses to mechanical load in vitro involve COX-2 and constitutive NOS"Connective Tissue Research. 45. 1-10 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Singh ATK: "Regulation of parathyroid hormone-stimulated phospholipase D in UMR-106 cells"Journal of Bone and Mineral Research. 18. 1453-1460 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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