| Project/Area Number |
13672138
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
矯正・小児・社会系歯学
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| Research Institution | Tohoku University |
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Principal Investigator |
IGARASHI Kaoru Tohoku University, Graduate School of Dentistry, Professor, 大学院・歯学研究科, 教授 (70202851)
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| Co-Investigator(Kenkyū-buntansha) |
SAEKI Shuichi Tohoku University, Graduate School of Dentistry, Instructor, 大学院・歯学研究科, 助手 (60271954)
WOO Je-tae Chubu University, College of Bioscience and Biotechnology, Associated Professor, 応用生物学部, 助教授 (20272693)
三谷 英夫 東北大学, 大学院・歯学研究科, 教授 (50014220)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Small G Protein / Rho / Mechanical Stimulus / Bone / Bone Formation / Osteoblast / International Exchange of Researcher / United States of America |
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| Research Abstract |
Mechanical stimulus is an essential factor for the maintenance of bone homeostasis. It is important to clarify the signal transduction pathway of mechanical stimulation (mechanotransduction pathway) leading to bone formation. In the present study, we investigated the role of Rho, a small G protein that regulates cellular configuration, in the mechanotransduction using pharmacological approach.
1.Development of a micromechanical testing device and establishment of the assay system
We developed an improved loading device capable of delivering loads with milli-Newton resolution and micrometer displacements to viable bone explants and determined conditions of loading which give the maximum anabolic responses.
2.Pharmacological study using a tissue culture system and the micromechanical testing device
It was suggested that small G proteins of Rho family might act as a negative regulator in the mechanotransduction pathway that leads to bone formation.
3.Pharmacological study using a cell culture system and the Flexercell Strain Unit
It was suggested that small G proteins of Rho family are involved in the nitric oxide production by osteoblasts. However, it is not known whether they have any role in the signal transduction of a cyclic tensional stimulus in osteoblastic cells.
4.Future prospect
Although the present results suggest that small G proteins of Rho family, are involved in the mechanotransduction pathway in bone, their role remains unclear and roles of the other families of small G proteins are not known. Further studies using molecular biological approach as well as pharmacological approach would be required to solve these questions.
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