| Project/Area Number |
13672145
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
矯正・小児・社会系歯学
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| Research Institution | Osaka University |
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Principal Investigator |
TANAKA Muneo (2002) Osaka University, Dental Hospital, Assistant Professor, 歯学部附属病院, 講師 (90263300)
片岡 宏介 (2001) 大阪大学, 大学院・歯学研究科, 助手 (50283792)
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| Co-Investigator(Kenkyū-buntansha) |
SHIZUKUISHI Satoshi Osaka University, Graduate School of Dentistry, Professor, 大学院・歯学研究科, 教授 (00028789)
KUBONIWA Masae Osaka University, Graduate School of Dentistry, Research Associate, 大学院・歯学研究科, 助手 (00303983)
田中 宗雄 大阪大学, 大学院・歯学研究科, 助手 (90263300)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2002: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | statherin / F. nucleatum / bindingsite / Fusobacterium nucleatum / 結合部位 |
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| Research Abstract |
The purpose of this study was to identify the binding sites of salivary, statherin (amino acid residues 1 to 43) to Fusobacterium nucleatum IN our preliminary experiments, we showed that statherin had the strangest binding ability to 125Ilabeled F. nucleatum (125IF. nudeatum) in the various salivary proteins. To determine the binding site of statherin in the binding of 125I-F. nucleatum to stalherincoated hydroxyapatite (sHAP) beads, we performed inhibition assays by using synthetic analogous peptides. As a result, peptide 19-26, 32-39 inhibited F. nudeatum binding to sHAP beads by 77 , 68% respectively. Although, peptide 1-6 , 6-14 did not inhibit. Furthermore, synthetic peptides were prepared by serial deletions of individual residues from N and C termini of peptide GPYQPVPE and QPYQPQYQ. Peptide YQFVPE and PYQPQYQ were found as inhibitory as peptide GPYQPVPE and QPYQPQYQ, respectively. However, further deletions of the residues fiom N and C termini resulted in significant loss of the inhibitory effect. These results suggest that peptide YQPVPE and PYQPQYQ may be the minimal active segment for binding to F. nucleatum which is considered to be related with senile pneumonia
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