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Tissue patterning mechanisms of facial prominences in chicken embryo

Research Project

Project/Area Number 13672146
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 矯正・小児・社会系歯学
Research InstitutionNara Medical University

Principal Investigator

KAWAKAMI Masayoshi  Nara Medical University, Department of Oral and Maxillofacial Surgery, Research Associate, 医学部, 助手 (20244717)

Co-Investigator(Kenkyū-buntansha) TAKADA Kenji  Osaka University Graduate School of Dentistry, Department of Orthodontics and Dentofacial Orthopedics, Professor, 大学院・歯学研究科, 教授 (50127247)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥2,800,000 (Direct Cost: ¥2,800,000)
Keywordschick embryo / frontonasal mass / cleft lip / Msx-1 / craniofacial / epithelial / facial primodial / chondrogenesis / Msx-1 / embryo / limb bud / 顔面骨格 / 軟骨形成
Research Abstract

Local patterning events occur within the frontonasal mass, one of the facial prominences that surrounds the embryonic oral cavity. In the chicken embryo, the upper beak is primarily formed by the frontonasal mass, the prominence that lies between the nasal slits. In the present study we dissociate mesenchyme from the lateral and central thirds of the frontonasal mass and compare the ability to form a single cartilage rod. We find that central third cell pellets combined with facial epithelium have a tendency to form a straight cartilage rod whereas the cells from the lateral third produce more branched outgrowths. In the reconstituted cell pellets with lateral and central cells mixed together, the central cells aggregate in the cartilage condensations while lateral cells are distributed throughout the graft. The central cell pellets contain greater numbers of potential chondrogenic cells than lateral cell pellets.
To see whether medio-lateral patterning is re-established in the cell pellets, we used Msx-2 as a marker for the lateral mesenchyme at stage 24. Central cell pellet recombinations do not express the gene or turn on bilateral expression. Grafts of lateral cells maintain and up-regulate Msx-2 expression 48 hours after grafting, but are not able to establish bilateral symmetry. The cell pellets that are mixtures of central and lateral third mesenchyme often express three patches of Msx-2 transcripts. Thus the central region of the frontonasal mass probably contains the majority of prechondrogenic cells at stage 24 and these cells have a tendency to segregate from the surrounding non-chondrogenic mesenchyme. Msx-2 may help to set up the differences between central and lateral mesenchyme prior to cell differentiation.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Kawakami M, Richman JM: "Local patterning with chicken frontonasal mass"Journal of Dental Research. 80(Special Issue). 634 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Lee SH, Fu KK, Hui JN, Richman JM: "Noggin and retinoic acid transform the identity avian facial prominences"Nature. 414(6866). 909-912 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hyomoto M, Kawakami M, Hanamoto S, Kirita T: "A clinicopathologic study of 184 dentigerous cysts"Journal of Nara Medical Association. 52(5). 181-187 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 俵本眞光, 川上正良, 華本真悟, 桐田忠昭: "含歯性嚢胞における関連埋伏歯とその萌出に関する研究"奈良医学雑誌. 52(6). 229-235 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ashique AM, Fu K, Richman JM: "Endogeneous bone morphogenic proteins regulate outgrowth and epithelial survival during avian lip fusion"Development. 129. 4667-4660 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kawakami M, Yagi T, Takada K: "Maxillary expansion and protraction in correction of a midface retrusion in a complete unilateral cleft lip and palate"Angle Orthodontist. 72. 355-361 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ashique AM, Fu K, Richman JM: "Signaling via type IA and type IB bone morphogenic protein receptors (BMPR) regulates intramembranous bone formation chondrogenesis and feather formation"International Journal of Developmental Biology. 46. 243-253 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yagi T, Kawakami M, Takada K: "Surgical orthodontic correction of acromegaly with mandibular prognathism"Angle Orthodontist. 73(In press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kawakami M, Richman JM: "Local patterning with chicken frontonasal mass"Journal of Dental Research. 80 (Special Issue). 634 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Lee SH, Fu KK, Richman JM: "Noggin and retinoic acid transform the identify avian facial prominences"Nature. 414(6866). 909-912 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hyomoto M, Kawakami M, Hanamoto S, Kirita T: "A clinicopathologic study of 184 dentigerous cysts"Journal of Nara Medical Association. 52. 181-187 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hyomoto M, Kawakami M, Hanamoto S, Kirita T: "A study of the impacted tooth associated with dentigerous cyst"Journal of Nara Medical Association. 52. 229-235 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ashique AM, Fu KK, Richman JM: "Endogeneous bone morphogenic proteins regulate outgrowth and epithelial survival avian lip fusion"Development. 129. 4647-4600 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kawakami M, Yagi T, Takada K: "Maxillary expansion and protraction in correction of a midface retrusion in a complete unilateral cleft lip and palate"Angle Orthodontist. 72. 355-361 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ashique AM, Fu KK, Richman JM: "Signaling via type IA and type IB bone morphogenic protein receptors (BMPR) regulates intramembranous bone formation, chondrogenesis and feather formation"International Journal of Developmental Biology. 46. 243-253 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yagi T, Kawakami M, Takada K: "Surgical orthbdontic correction of acromegaly with mandibular prognathism"Angle Orthodontist. 73, in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ashique AM, Fu K, Richman JM: "Endogeneous bone morphogenic proteins regulate outgrowth and epithelial survival during avian lip fusion"Development. 129. 4647-4660 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kawakami M, Yagi T, Takada K: "Maxillary expansion and protraction in correction of a midface retrusion in a complete unilateral cleft lip and palate"Angle Orthodontist. 72. 355-361 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ashique AM, Fu K, Richman JM: "Signaling via type IA and type LB bone morphogenic trotein receptors (BMPR) regulates intramembranous bone formation chondrogenesis and feather formation"International Journal of Developmental Biology. 46. 243-253 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yagi T, Kawakami M, Takada K: "Surgical orthodontic correction of acromegaly with mandibular prognathism"Angle Orthodontist. 73(in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kawakami M, Richman JM: "Local patterning with chicken frontonasal mass"Journal of Dental Research. 80(Special Issue). 634 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Lee SH, Fu KK, Hui JN, Richman JM: "Noggin and retinoic acid transform the identity avian facial prominences"Nature. 414(6866). 909-912 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hyomoto M, Kawakami N, Hanamoto S, Kirita T: "A clinicopathologic study of 184 dentigerous cysts"Journal of Nara Medical Association. 52(5). 181-187 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 俵本眞光, 川上正良, 華本真悟, 桐田忠昭: "含歯性襄胞における関連埋伏歯とその萌出に関する研究"奈良医学雑誌. 52(6). 229-235 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kawakami M, Yagi T, Takada K: "Maxillary expansion and protraction in correction of a midface retrusion in a complete unilateral cleft lip and palate"Angle Orthodontist. (in press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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