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CTGF gene delivery for enhancement the healing of mandibular ramus fracture

Research Project

Project/Area Number 13672152
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 矯正・小児・社会系歯学
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

KURODA Shingo  Okayama University Graduate School of Medicine and Dentistry, Research assistant, 大学院・医歯学総合研究科, 助手 (40332796)

Co-Investigator(Kenkyū-buntansha) KURATANI Tsuyoshi  Okayama University Graduate School of medicine and dentistry, Research assistant, 大学院・医歯学総合研究科, 助手 (90311802)
KAMIOKA Hiroshi  Okayama University Dental Hospital, Assistant Professor, 歯学部附属病院, 講師 (80253219)
YAMAMOTO Teruko (TAKANO Teruko)  Okayama University Graduate School of Medicine and Dentistry, Professor, 大学院・医歯学総合研究科, 教授 (00127250)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2002: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2001: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywordscondylar fracture / in situ hybridization / immunohisto-chemistry / gene introduction / HVJ-liposome / ラット / connective tissue growh factor / Collagen type I / 骨折治癒 / connective tissue growth factor
Research Abstract

The purpose of the present study was to investigate the CTGF gene delivery for enhancing the repair of fractured condylar and mandibular ramus. Using six-week-old wistar rat, 12 hours, 1, 3, 5, 7, and 14 days after the fracture, mandible including fractured condylar and ramus were dissected and decalcified with EDTA. After embedded in paraffin, 5 μ, β horizontal and sagittal sections were made. RNA probes of CTGF, collagen type I, II, III, X, Cbfal and TGF 0 were used for in situ hybridization to examine the expression of these factors during fracture healing. CTGF were expressed not only hypertrophic but hyperplastic chondrocyte in the healing site and this expression was changed in the time course. The present findings suggest that CTGF may play an important roll for repair and healing in condylar fracture and this model is useful as a model of fracture healing.
Following experiments were performed in vitro and in vivo, to examine the optimum conditions of HVJ liposome method for the condylar fracture model. (1) Luciferase gene was delivered to mesenchymal cell, isolated from the bone marrow in mouse femur, by using HVJ-liposome method in vitro. Luciferase reporter assay was performed to evaluate delivery rate. (2) After the fracture, β-galactosidase gene was delivered by using HVJ-liposome method. Three days after gene introduction, X-gal staining was performed to evaluate the delivery rate. These pilot studies indicated that the gene delivery by using HVJ-liposome was efficient to the condylar fracture model.
CTGF gene delivered by using HVJ-liposome in rat is performed and both in situ hybridization and immuno-histochemistry were used to analyze the expression of CTGF during fracture healing. We are preparing the paper about these observations to submit to the international journals, and some of them have been accepted.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Fukunaga T: "Immunolocalization and gene expression of connective tissue growth factor during healing of mandibular ramus fracture"J Okayama Dent Soc. 21・1. 143-154 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshimichi G: "CTGF/Hcs24 interacts with the cytoskeletal protein actin in chondrocytes"Biochem Biophys Res Commun. 299・5. 755-761 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yoshimichi G: "CTGF/Hcs24 induces chondrocyte differentiation through p38 mitogen-activated protein kinase(p38MAPK), and proliferation through p44/42 MAPK/extracellular-signal regulated kinase(ERK)"Eur J Biochem. 268・23. 6058-6065 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamashiro, T.: "Mechanical stimulation induces CTGF expression in rat osteocytes"Journal of Dental Research. 80・2. 461-465 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kamioka, H.: "A three-dimensioned distribution of osteocyte processes revealed by the combination of CLS and DIC microscopy"Bone. 28・2. 145-149 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamashiro T: "Gene and Protein Expression of Brain-Derived Neurotrophic Factor and TrkB in Bone and Cartilage"Bone. 28・4. 404-409 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takano-Yamamoto T: "Orthodontics in the 21st Century-Where are We Now? Where Are We Going?"Osaka University Press. 216 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kamioka H, Honjo T, Takano-Yamamoto T: "A three-dimensional distribution of osteocyte processes revealed by the combination of confocal laser scanning microscopy and differential interference microscopy"Bone. 28(2). 145-149 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sugimoto T, Takeyama A, Fujita M, Ichikawa H, Takano-Yamamoto T: "Peripheral neurogliai death induced by cisplatin administration in neuborn rats"Neuro Report. 12(1). 137-140 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ye Q, Ohsaki K, Li K, Li D-J, Zhu C-S, Ogawa T, Tenshin S, Takano-Yamamoto T: "Histological reaction to hydroxyapatite in the middle ear of rats"Auris Nasus Larynx. 28(2). 131-136 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tsuboi Y, Nakanishi T, Takano-Yamamoto T, Miyamoto M, Yamashiro T, Takigawa M: "Mitogenic effects of neurotrophines on a periodontal ligament cell line"J Dent Res. 80(3). 881-886 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamashiro T, Fukunaga T, Kobashi N, Kamioka H, Nakanishi T, Takigawa M, Takano-Yamamoto T: "Mechanical stimulation induces CTGF expression in rat osteocytes"J Dent Res. 80(2). 461-465 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamashiro T, Takano-Yamamoto T: "Influences of ovariectomy on experimental tooth movement in the rat"J Dent Res. 80(9). 1858-1861 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamashiro T, Fukunaga T, Kabuto H, Ogawa N, Takano-Yamamoto T: "Activation of the Bulbospinal Serotonergic System during Experimental Tooth Movement in the Rat"J Dent Res. 80(9). 1854-1857 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamashiro T, Fukunaga T, Yamashita K, Kobashi N, Takano-Yamamoto T: "Gene and Protein Expression of Brain-Derived Neurotrophic Factor and TrkB in Bone and Cartilage"Bone. 28(4). 404-409 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ichikawa H, Yamashita K, Takano-Yamamoto T, Sugimoto T: "Osteopontin-immunoreactivity in the rat trigeminal ganglion and trigeminal sensory nuclei"Brain Res. 919(1). 147-154 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yosimichi G, Nakanishi T, Nishida T, Hattori T, Takano-Yamamoto T, Takigawa M: "CTGF/Hcs24 induces chondrocyte differentiation through p38 mitogen-activated protein kinase (p38MAPK), and proliferation through p44/42 MAPK/extracellular-signal regulated kinase (ERK)"Eur J Biochem. 268(23). 6058-6065 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yosimichi G, Kubota S, Hattori T, Nishida T, Nawachi K, Nakanishi T, Kamada M, Takano-Yamamoto T, Takigawa M: "CTGF/Hcs24 interacts with the cytoskeletal protein actin in chondrocytes"Biochem Biophys Res Commun. 299(5). 755-761 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fukunaga T, Takano-Yamamoto T: "Immunolocalization and gene expression of connective tissue growth factor during healing of mandibular ramus fracture"J Okayama Dental Soc. 21(1). 143-154 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takano-Yamamoto T, Ed. Kenji Takada and William R. Proffit: "Introductory Remarks : Current views of molecular biology in Orthodontics, Orthodontics in the 21st Century - Where are We Now? Where Are We Going?"Osaka University Press. 81-88 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamashiro T, Takano-Yamamoto T, Ed. Kenji Takada and William R. Proffit: "Pain and Orthodontics, Orthodontics in the 21st Century - Where are We Now? Where Are We Going?"Osaka University Press. 101-107 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Miyamoto M, Takano-Yamamoto T, Ed. Kenji Takada and William R. Proffit: "Molecular diagnosis of Japanese familial cases of Crouzon syndrome, Orthodontics in the 21st Century - Where are We Now? Where Are We Going?"Osaka University Press. 165-170 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kamioka, H.: "A three-dimensional distribution of osteocyte processes revealed by the combination of CLS and DIC microscopy"Bone. 28・2. 145-149 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamashiro, T.: "Mechanical stimulation induces CTGF expression in rat osteocytes"Journal of Dental Research. 80・2. 461-465 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamashiro, T.: "Inferior alveolar nerve transection inhibits increase in osteoclast appearance"Bone. 26・6. 663-669 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2022-01-20  

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