• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

A new regenerative therapy of periodontal tissue using periostium and molecular chaperones induction

Research Project

Project/Area Number 13672187
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Periodontal dentistry
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

KAWAGUCHI Hiroyuki  Hiroshima University, Graduate School of Biomedical Sciences, Associate Professor, 大学院・医歯薬学総合研究科, 助教授 (10224750)

Co-Investigator(Kenkyū-buntansha) MIZUNO Noriyoshi  Hiroshima Univ. Graduate Sch. Biomed. Sci., Research Associate, 大学院・医歯薬学総合研究科, 助手 (60325181)
SHIBA Hideki  Hiroshima Univ. Graduate Sch. Biomed. Sci., Research Associate, 大学院・医歯薬学総合研究科, 助手 (60260668)
OGAWA Tetsuji  Hiroshima Univ. Dent. Hospital, Professor, 歯学部附属病院, 教授 (50112206)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsmolecular chaperones / stress proteins / gingival fibroblast / gingival epithelial cell / periodontal regeneration / periostium
Research Abstract

This research has shown clearly that constitutively proteins are inducted at 37 ℃, 3 hours heat treatment, and that Hsp (heat shock proteins) of stress proteins (chaperon molecule) are inducted at 42℃, 3 hours in both a human gingival epithelium cell (HGK) and a human gingival fibroblast (HGF).
After acting A.actinomycetemcomitans on HGK, expressions of stress proteins were analyzed by RT-PCR method and the western blotting method. Expression of Hsp27, Hsp60, and Hsp70 was recognized. These expressions were found in cell layer. As for Hsp90, expression was not accepted by heat treatment of 37℃ nor 42℃ at HGK. When Geranylgeranylacetone, inductive agent of chaperon molecule, was made to act on HGK, expression of mRNA of COX-2 and IL-8 was induced. In HGK, even 37℃ and heat treatment of 3 hours expression of mRNA of COX-2 was increased. In the heat treatment to HGF, expression of Hsp104 was not detected at 37℃ or 42℃. On the other hand, expression of Hsp90 increased at 42℃. Expression of Hsp70 and Hsp40 also increased at 42℃ heat treatment similarly.
It is necessary to catch the influence of heat treatment in periosteum connective tissue in vivo based on the effect of warm temperature to gingival tissue.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Sakata M, Shiba H, Kawaguchi H et al.: "Osteoprotegerin levels increased by interleukin-1beta in human periodontal ligament cells are suppressed through prostaglandin E2 synthesized de novo"Cytokine. 18(3). 133-139 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fujita T, Shiba H et al.: "SPARC stimulates the synthesis of OPG/OCIF, MMP-2 and DNA in human periodontal ligament cells"Oral Pathol. Med. 31(6). 345-352 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fujita T, Shiba H et al.: "Effects of transforming growth gactor-b1 and fibronectin on SPARC expression in cultures of human periodontal ligament cells"Cell Biol. Int. 26(12). 1065-1072 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hasegawa N, Kawaguchi H et al.: "Immunohistochemical characteristics of epithelial cell rests of Malassez during cementum repair"J Periodont Res. 38. 51-56 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sakata M, Shiba H, Kawaguchi H., et.al.: "Osteoprotegerin levels increased by interleukin-1 beta in human periodontal ligament cells are suppressed through prostaglandin E2 synthesized de novo"Cytokine. 18. 133-139 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fujita T, Shiba H, Kurihara H et.al.: "SPARC stimulates the synthesis of OPG/OCIF, MMP-2 and DNA in human periodontal ligament cells"Oral Pathol.Med.. 31. 345-352 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fujita T, Shiba H, Kurihara H et.al.: "Effects of transforming growth gactor-b1 and fibronectin on SPARC expression in cultures of human periodontal ligament cells"Cell Biol.Int.. 26. 1065-1072 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hasegawa N, Kawaguchi H et.al.: "Immunohistochemical characteristics of epithelial cell rests of Malassez during cementum repair"J Periodont Res. 38. 51-56 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sakata M, Shiba H, Kawaguchi H et al.: "Osteoprotegerin levels increased by interleukin-1 beta in human periodontal ligament cells are suppressed through prostaglandin E2 synthesized de novo"Cytokine. 18(3). 133-139 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fujita T, Shiba H et al.: "SPARC stimulates the synthesis of OPG/OCIF, MMP-2 and DNA in human periodontal ligament cells"Oral Pathol. Med. 31(6). 345-352 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fujita T, Shiba H et al.: "Effects of transforming growth gactor-b1 and fibronectin on SPARC expression in cultures of human periodontal ligament cells"Cell Biol. Int. 26(12). 1065-1072 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hasegawa N, Kawaguchi H et al.: "Immunohistochemical characteristics of epithelial cell rests of Malassez during cementum repair"J Periodont Res. 38. 51-56 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 河口 浩之: "自家骨髄間葉系幹細胞を用いた歯周治療の可能性"日本歯周病学会会誌. 44(秋季特別号). 60 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Uchida U, Shiba H et al.: "Expression of IL-1 and IL-8 by human gingival epithelial cells in response to Actinbacillus actinomycetemcomitans"Cytokine. 14. 152-161 (2001)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi