• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Liver-selective delivery of 5-fluorouracil utilizing the absorption on the liver surface in rats

Research Project

Project/Area Number 13672255
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical pharmacy
Research InstitutionNagasaki University

Principal Investigator

NAKAMURA Junzo  Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (30115901)

Co-Investigator(Kenkyū-buntansha) NAKASHIMA Mikiro  Nagasaki University, School of Medicine, Department of Hospital Pharmacy, Associate Professor, 医学部附属病院, 助教授 (00260737)
SAKAEDA Toshiyuki  Kobe University, Department of Hospital Pharmacy, School of Medicine, Associate Professor, 医学部附属病院, 助教授 (00304098)
NISHIDA Koyo  Graduate School of Biomedical Sciences, Associate Professor, 大学院・医歯薬学総合研究科, 助教授 (20237704)
MUKAI Takahiro  Kyoto University Graduate School of Medicine, Assistant Professor, 医学研究科, 助手 (30284706)
川上 茂  長崎大学, 薬学部, 助手 (20322307)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2001: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsLiver / Drug targeting / Liver surface application / 5-Fluorouracil / Drug delivery system / Rat / Anticancer agent / Cancer chemotherapy / 抗癌剤
Research Abstract

The liver plays an important role in maintaining homeostasis, thus many liver diseases such as hepatitis, cirrhosis and hepatoma are lethal. Although the anticancer drugs are administered orally, intravenously and intraarterially in the chemotherapy of hepatoma, the anticancer drugs are distributed to the whole body via the blood stream following the administration, leading to the inadequate liver- and lobe-selective drug delivery. The present study was undertaken to elucidate the liver- and lobe-selective delivery of 5-fluorouracil (5-FU) following the liver surface application in rats. We selected an experimental system utilizing a cylindrical diffusion cell attached to the liver surface. After liver surface application of 5-FU, 5-FU was absorbed approximately 70 % in 360 min. A semi-log plot of the remaining amount of 5-FU in the diffusion cell gave a straight line. The 5-FU concentration at lobes of the liver after intravenous administration was similar. In contrast, 5-FU was site-selectively delivered in the liver after liver surface application. From these results, we demonstrated that the absorption of 5-FU on the liver surface in rats is explained mostly by passive diffusion. Furthermore, it is elucidated the liver- and lobe-selective delivery of 5-FU following the liver surface application in rats.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Koyo Nishida: "Effect of instillation method on the absorption of phenolsulphonphthalein as a model drug from the liver and small intestinal serosal surface in rats"Journal of Pharmacy and Pharmacology. 53. 1341-1346 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Koyo Nishida: "Absorption characteristics of model compounds with different molecular weights from the serosal caecal surface in rats"Journal of Pharmacy and Pharmacology. 54. 1005-1009 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shigeru Kawakami: "Kidney-and site-selective delivery of 5-fluorouracil utilizing the absorption on the kidney surface in rats"Biological & Pharmaceutical Bulletin. 25. 928-930 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shigeru Kawakami: "Liver-and lobe-selective gene transfection following the instillation of plasmid DNA to the liver surface in mice"Biochemical and Biophysical Research Communications. 294. 46-50 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takahiro Mukai: "A novel method for preparation of animal models of liver damage : Liver targeting of carbon tetrachloride in rats"Biological & Pharmaceutical Bulletin. 25. 1494-1497 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ryu Hirayama: "Development of the liver-and lobe-selective nonviral gene transfer following the instillation of naked plasmid DNA using catheter on the liver surface in mice"Pharmaceutical Research. 20. 328-332 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Koyo Nishida: "Effect of instillation method on the absorption of phenolsulphonphthalein as a model drug from the liver and small intestinal serosal surface in rats"Journal of Pharmacy and Pharmacology. 53(10). 1341-1346 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Koyo Nishida: "Absorption characteristics of model compounds with different molecular weights from the serosal caecal surface in rats"Journal of Pharmacy and Pharmacology. 54(7). 1005-1009 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shigeru Kawakami: "Kidney- and site-selective delivery of 5-fluorouracil utilizing the absorption on the kidney surface in rats"Biological & Pharmaceutical Bulletin. 25(7). 928-930 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shigeru Kawakami: "Liver- and lobe-selective gene transfection following the instillation of plasmid DNA to the liver surface in mice"Biochemical and Biophysical Research Communications. 294(1). 46-50 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takahiro Mukai: "A novel method for preparation of animal models of liver damage : Liver targeting of carbon tetrachloride in rats"Biological & Pharmaceutical Bulletin. 25(11). 1494-1497 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ryu Hirayama: "Development of the liver- and lobe-selective nonviral gene transfer following the instillation of naked plasmid DNA using catheter on the liver surface in mice"Pharmaceutical Research. 20(2). 328-332 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Koyo Nishida: "Absorption characteristics of model compounds with different molecular weights from the serosal caecal surface in rats"Journal of Pharmacy and Pharmacology. 54・7. 1005-1009 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shigeru Kawakami: "Kidney-and site-selective delivery of 5-fluorouracil utilizing the absorption on the kidney surface in rats"Biological & pharmaceutical Bulletin. 25・7. 928-930 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shigeru Kawakami: "Liver-and lobe-selective gene transfection following the instillation of plasmid DNA to the liver surface in mice"Biochemical and Biophysical Research Communications. 294・1. 46-50 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takahiro Mukai: "A novel method for preparation of animal models of liver damage : liver targeting of carbon tetrachloride in rats"Biological & Pharmaceutical Bulletin. 25・11. 1494-1497 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ryu Hirayama: "Development of the liver-and love-selective nonviral gene tranfer following the instillation of naked plasmid DNA using catheter on the liver surface in mice"Pharmaceutical Research. 20・2. 328-332 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Koyo Nishida: "Effect of instillation method on the absorption of phenolsulphonphthalein as a model drug from the liver and small intestinal serosal surface in rats"Journal of Pharmacy and Pharmacology. 53巻10号. 1341-1346 (2001)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi