• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The mechanistic study of the interaction between α-tocopherol and biological oxidative radicals

Research Project

Project/Area Number 13672262
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical pharmacy
Research InstitutionKyoritsu College of Pharmacy

Principal Investigator

ITOH Yoshiko  Kyoritsu College of Pharmacy, Department of Physical Chemistry, Assistant Professor, 薬学部, 助手 (40228036)

Co-Investigator(Kenkyū-buntansha) KANAZAWA Hideko  Kyoritsu College of Pharmacy, Department of Physical Chemistry, Associate Professor, 薬学部, 助教授 (10240996)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsα-Tocopherol / Nitric oxide / Oxidative stress / Radical / Antioxidant
Research Abstract

α-Tocopherol (α-Toc) is a ubiquitous antioxidant in biological systems and protects biological molecules from the oxidation induced by various kinds of active oxygens. Nitric oxide (NO), which is a biological oxidative radical, has been known to fill the roles of mediators in many physiological phenomena. In addition, NO and its oxygenated derivatives bring about cytotoxicity when they are generated in relatively high concentration.
Since these active species derived from NO are supposed to give oxidative damages to the body, it is important to investigate their interaction with α-Toc. In this study, the reactions of NO with α-Toc was carried out using limited amounts of NO and molecular oxygen in order to reveal a reactivity and a productive interaction between them. Since vitamin E exists in lipid part of the biological system, these reactions were carried out in various organic solvents. The high yields of five major products, αptocquinone (α-TQ) (max21%), tocored (max52%), 5-formyl-7, 8-dimethyltocol (5-FDT) (max29%), 2, 3-dihydro-7a-(3-hydroxy-3,7,11,15-tetramethyl-6, 10, 14-hexadecatrienyl)-3, 5, 6, 9, 10, 11a-hexamethyl-3(4, 8, 12-trimethyl-3, 7, 11-tridecatrienyl)-1H-pyrano[2,3a]xanthene-8(7a-H), 11(11a-H)-dione(Toc-dimer) (max10%), 2-(3-hydroxy-3, 7, 11, 15-tetramethylhexadecyl)-5, 6-dimethyl-3-nitrosoxymethyl-[1, 4] benzoquinone (3-Nitrite), were obtained from the reaction of α-Toc. These were analyzed by the gradient elution method with the high performance liquid chromatograhy.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] 永田佳子, 金澤秀子, 望月正隆, 松島美一, 松本茂信: "α-TocopherolとNOの酸素存在下での反応における溶媒効果について"ビタミンE研究の進歩X. 10巻. 1-6 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Nagata., H.Kanazawa., Mochizuki., Y.Matsushima., S.Matsumoto.: "Effects of solvents of the reaction of α-tocopherol and nitric oxide in the presence of oxygen"Bitaminkenkyu no sinpo. 10. 1-6 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 永田佳子, 金澤秀子, 望月正隆, 松島美一, 松本茂信: "α-TocopherolとNOの酸素存在下での反応における溶媒効果について"ビタミンE研究の進歩X. 10巻. 1-6 (2002)

    • Related Report
      2002 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi