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Preparation of transgenic mouse of PHBP which is activated specifically in hepatic injury

Research Project

Project/Area Number 13672298
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionShowa University

Principal Investigator

CHOI-MIURA Nam-Ho  School of Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (10146904)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsPHBP / serine protease / hapatic failure / urokinase / factor VII / transgenic mouse / セソンプロテアーゼ
Research Abstract

1) Construction of transgene
Mouse PHBP cDNA coding region (1.6kbp) was ligated in pCAGGA vector which has cytomegalovirus enhancer, chiken β-actin promoter and rabbit β-globin 3'-flanking sequence in the sense direction and the transgene was named pCAPHGS. To confirme the expression of PHBP from the transgene, pCAPHGS was transfected in COS-7 and CHO cells using LIPOFECTAMINE2000 (Invitrogen) and the expression of PHBP was analyzed by western- blot analysis. The results indicated clearly the expression of PHBP from pCAPHGS.
2) Preparation and analysis of transgenic mouse
pCAPHGS was microinjected into the fertilized oocytes from C57BL/6 and the eggs were transplanted in the oviduct of female mouse. We prepared the genomic DNAs from the tails of newborne mice and analyzed them wheter they contained the transgene. As the result, we obtained 1 transgenic mouse (F0). In comparison of wild-type and F1 mice, we failed to detect any diffecences in body mass, out-looking, behavioral action and sex ratio, however, the number of newborn mice from F1 was less than from wild-type. In addition, homozygotes from F1 parents died within 1-2 days.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] M.Yoda-Murakami: "Change in expression of adiponectin in carbon tetrachloride-administrated mouse liver"Biochem. Biophys. Res. Commun.. 285. 372-377 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Ishikawa et al.: "Immunolocalization of apolipoproteins in aortic atherosclerosis in American youths and young adults"Atherosclerosis. 158. 215-225 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Tozaki et al.: "Population study and validation of paternity testing for thoroughbred horses by 15 microsatellite loci"J. Vet. Med. Sci.. 63. 1191-1197 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Tozaki et al.: "SNP analysis of the IHRP gene by a fluorescence-adapted SSCP method"BMC Medical Genetics. 3. 1-8 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Matsuura et al.: "Increased expression of vascular endothelial growth factor in placentas of p57Kip2 null embryos"FEBS Lett.. 532. 283-288 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Ishikawa et al.: "Changes in the distribution pattern of adiponectin in myocardial remodelling after ischaemic injury"Histopathology. 42. 43-52 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M. Yoda-Murakami et al.: "change in expression of adiponectin in carbon tetrachloride-administrated mouse liver"Biochem. Biophys. Res. Commun.. 285. 372-377 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y. Ishikawa et al.: "Immunolocalization of apolipoproteins in aortic atherosclerosis in American youths and young adults"Atherosclerosis. 158. 215-225 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Tozaki et al.: "Populatin study and validation of paternity testing for thoroughbred horses by 15 microsatellite loci"J.Vet. Med. Sci.. 63. 1191-1197 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Tozaki et al.: "SNP analysis of the IHRP gene by a fluorescence-adapted SSCP method"BMC Medical Genetics. 3. 1-8 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Matsuura et al.: "icreased expression of vascular endothelial growth factor in placentas of p57Kip2 null mbryos"FEBS Lett.. 532. 283-288 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y. Ishikawa et al.: "Changes in the distribution pattern of adiponectin in myocardial remodelling after ischaemic injury"Histopathology. 42. 43-52 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Yoda, Murakami et al.: "Change in expression of adiponectin in carbon tetrachloride-administrated mouse liver"Biochem. Biophys. Res. Commun.. 285. 372-377 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Y.Ishikawa et al.: "Immunolocalization of apolipoproteins in aortic atherosclerosis in American youths and young adults"Atherosclerosis. 158. 215-225 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Tozaki et al.: "Poplatin study and validation of paternity testing for thoroughbred horses by 15 microsatellite loci"J. Vet. Med. Sci.. 63・11. 1191-1197 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Tozaki et al.: "SNP analysis of the IHRP gene by a fluorescence-adapted SSCP method"BMC Medical Genetics. 3・6. 1-8 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Matsuura et al.: "Increased expression of vascular endothelial growth factor in placentas of p57Kip2 null embryos"FEBS Lett.. 532. 283-288 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Y.Ishikawa et al.: "Changes in the distribution pattern of adiponectin in myocardial remodelling after ischaemic injury"Histopathology. 42. 43-52 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nam-Ho Choi-Miura: "Proteolytic activation and inactivation of the serine protease activity of plasma hyaluronan binding protein"Biol. Pharm. Bull.. 24(5). 448-452 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nam-Ho Choi-Miura: "Hepatic injury-specific conversion of mouse plasma hyaluronan binding protein to the active hetero-dimer form"Biol. Pharm. Bull.. 24(8). 892-896 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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