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Significance of Alteration of Phospholipase C-δ1 in the Brain Tissues through Oxidative Stress

Research Project

Project/Area Number 13672313
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionKyoto Pharmaceutical University

Principal Investigator

FUJIMOTO Sadaki  Department of Environmental Biochemistry, Kyoto Pharmaceutical University, Professor, 薬学部, 教授 (80090182)

Co-Investigator(Kenkyū-buntansha) SHIMOHAMA Shun  Department of Environmental Biochemistry, Kyoto Pharmaceutical University, Professor, 医学部, 助教授 (60235687)
TANINO Hiroko  Department of Environmental Biochemistry, Kyoto Pharmaceutical University, Assistant, 薬学部, 助手 (90298688)
NAGASAWA Kazuki  Department of Environmental Biochemistry, Kyoto Pharmaceutical University, Assistant Lecturer, 薬学部, 講師 (30228001)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsPhospholipase C isozyme / Phospholipase C-δ1 / Lipid peroxidation / Oxidative stress / Hyperoxia / Acrylonitrile / Rat heart / Rat cerebral cortex / Caspase family / イノシトールリン脂質代謝 / ラット / 脳 / アルツハイマー
Research Abstract

When oxidative stress is induced in the rat cerebral cortex through hyperoxia, the protein levels of phospholipase C (PLC)-β1 and -δ1 were significantly increased in the cerebral cortex, although that of PLC-γ1 was not altered. These results suggest that hyperoxia exerts oxidative stress on the rat cerebral cortex, and the protein levels of PLC-β1 and -δ1, but not PLC-γ1, were changed. Acrylonitrile (CAN) appears to induce astrocytomas through induction of oxidative stress on the rat brain selectively. Treatment of rats with CAN, but not hyperoxia, resulted in lipid peroxidation in the heart and cerebral cortex, and the effect on the latter was greater than on the former. In the CAN-treated cerebral cortex, significant increases in the PLC-β1 and -δ1 in the cytosol, and PLC-γ1 in the cytosolic and particulate fractions, and lysate were observed. In the rat heart, in which PLC-β1 could not be detected, PLC-γ1 and -δ1 were increased and decreased in the cytosolic and particulate fractions, respectively, by hyperoxia. In addition, the expression level of PLC-γ1 was decreased in the lysate by the treatment. In the heart treated with CAN, there was no change in the level of PLC-γ1, while PLC-δ1 was elevated in all fractions. We have demonstrated that oxidative stress induced by hyperoxia is apparently less than that induced by CAN administration in the heart as a peripheral tissue and the cerebral cortex as a central one. With both forms of oxidative stress, the protein levels of PLC isozymes were altered, but there was apparent difference in the altered protein levels between the different levels of oxidative stress, and between the organs. In order to obtain the information about contribution of PLC isozymes to the central nervous system development and aging, the expression of rat brain caspase family proteins in development and aging, and their subcellular distribution were also assessed using immunochemical detection.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Shun Shimohama et al.: "Differential subcellular localization of caspase family proteins in the adult rat brain"Neurosci.Lett.. 315. 125-128 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hiroko Tanino et al.: "Alteration of phsphpolipase C isozymes in rat cerebral cortex through hyperoxia"Biol.Pharm.Bull.. 24. 1241-1245 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shun Shimohama et al.: "Differential expression of rat brain caspase family proteins during development and aging"Biochem.Biophys.Res.Commun.. 289. 1063-1066 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kazuki Nagasawa et al.: "Effects of hyperoxia and acrylonitrile on the phospholipase C isozyme protein levels in rat heart and brain"Life Sci.. (in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shun Shimohama, Hiroko Tanino, Sadaki Fujimoto: "Differential subcellular localization of caspase family proteins in the adult rat brain"Neurosci. Lett.. 315. 125-128 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hiroko Tanino, Toshihiro Kusuda, Kazuki Nagasawa, Shun Shimohama, Sadaki Fujimoto: "Alteration of phsphpolipase C isozymes in rat cerebral cortex through hyperoxia"Biol. Pharm. Bull.. 24. 1241-1245 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shun Shimohama, Hiroko Tanino, Sadaki Fujimoto: "Differential expression of rat brain caspase family proteins during development and aging"Biochem. Biophys. Res. Commun.. 289. 1063-1066 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kazuki Nagasawa, Hiroko Tanino, Shun Shimohama, Sadaki Fujimoto: "Effects of hyperoxia and acrylonitrile on the phospholipase C isozyme protein levels in rat heart and brain"Life Sci.. in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shun Shimohama et al.: "Differential subcellular localization of caspase family proteins in the adult rat brain"Neurosci.Lett.. 315. 125-128 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hiroko Tanino et al.: "Alteration of phsphpolipase C isozymes in rat cerebral cortex through hyperoxia"Biol.Pharm.Bull.. 24(11). 1241-1245 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shun Shimohama et al.: "Differential expression of rat brain caspase family proteins during developmment and aging"Biochem.Biophys.Res.Commun.. 289. 1063-1066 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kazuki Nagasawa et al.: "Effects of hyperoxia and acrylonitrile on the phospholipase C isozyme protein levels in rat heart and brain"Life Sci.. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tanino H., et al.: "Alterations of phospholipase C isozymes in rat cerebral cortex through hyperoxia"Biol. Pharm. Bull.. 24(11). 1241-1245 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shimohama S., et al.: "Differential expression of rat brain caspase family proteins during development and aging"Biochem. Biophys. Res. Commun.. 289(5). 1063-1066 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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