• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Evaluation and improvement for poor bioavailability of aminoglycosides in the intestinal tract after oral administration

Research Project

Project/Area Number 13672330
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 医薬分子機能学
Research InstitutionDepartment of Pharmacokinetics, Kyoto Pharmaceutical University

Principal Investigator

HAKADA Kanji  Kyoto Pharmaceutical University Department of Pharmacokinetics Professor, 薬物動態, 教授 (30102106)

Co-Investigator(Kenkyū-buntansha) YOSHIKAWA Yukako  Kyoto Pharmaceutical University Department of Pharmacokinetics Associate Professor, 薬物動態, 助手 (30278444)
SHIBATA Nobuhito  Kyoto Pharmaceutical University Department of Pharmacokinetics Associate Professor, 薬物動態, 助教授 (60319449)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsGlycopeptides / Labrasol / Vitamine E TPGS / Vancomycin / Gentamicin / Bioavailability / LC-MS-MS / Pharmacokinetics / ゲンタマイシン / 自己乳化型製剤 / 低バイオアベイラビリティ / 腸管排泄機構
Research Abstract

We evaluated the mechanisms for poor permeability of aminoglycosides, vancomycin (VCM) and gentamicin (GM) in rats, and developed pharmaceutics of VCM and GM using an active surfactant, Labrasol. In addition, we developed a high sensitive LC/MS/MS method to determine VCM in plasma. The bioavailability of GM in self-micro-emulsifying drug delivery system (SMEDDS) using Labrasol increased by 50-100 folds as compared with a standard solution without Labrasol. The SMEDDS for VCM prepared by Labrasol and vitamin E TPGS at a weight ratio of 4:1 or addition of Eudragit also provided marked improvement in the VCM bioavailability after oral administration.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] YV Rama Prasad, SP Puthli, S Eaimtrakarn, M Ishida, et al.: "Enhanced intestinal absorption of vancomycin with Labrasol and D-α-tocopheryl PEG 1000 succinate in rats"International Journal of Pharmaceutics. 250. 181-190 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] N Shibata, M Ishida, YV Rama Prasad, et al.: "Highly sensitive quantitation of vancomycin in plasma samples using liquid chromatography-tandem mass spectrometry and oral bioavailability in rats"Journal of Chromatography B. 789. 211-218 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Z Hu, YV Pama Prasad, R Tawa, T Konishi, M Ishida et al.: "Diethyl ether fraction of Labrasol having a stronger absorption enhancing effect on gentamicin than Labrasol itself"International Journal of Pharmaceutics. 234. 223-235 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Z Hu, R Tawa, T Konishi, N Shibata, M Ishida et al.: "A novel emulsifir, Labrasol, enhances gastrointestimal absorption of gentamicin"Life Science. 69. 2899-2910 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] YV Rama Prasad, SP Puthli, S Eamtrakarn, M Ishida, et al.: "Enhanced intestinal absorption of Vancomycin with Labrasol and D-α tocopheryl PEG 1000 succunate in rats"Int J Pharmaceu.. 250. 181-190 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] N Shibata, M Ishida, YV Rama Prasad, et al.: "Highly sensitive quantitation of vancomicin in plasma samples using liquid chromatography-tandem mass spectrometry and oral bioavailability in rats"J Chromatogra B. 789. 211-218 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Z Hu, YV Rama Prasad, R Tawa, T Konishi, M Ishida, et al.: "Dirthyl ether fraction of Labrasol Hhaving a stronger absorption enhancing effect on gentamicin than Labrasol itself"Int J Pharmaceu.. 2002. 223-235 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Z Hu, R Tawa, T Konishi, N Shibata, et al.: "A Novel emulsifer, Labrasol, enhances gastro intestinal absorption of gentamicin"Life Science. 69. 2899-2910 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] YV Rama Prasad, SP Puthli, S Eaimtrakarn, M ishida, et al.: "Enhanced intestinal absorption of vancomycin with Labrasol and D-α-tocopheryl PEG 1000 succinate in rats"International Journal of Pharmaceutics. 250. 181-190 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] N Shibata, M Ishida, YV Rama Prasad, et al.: "Highly sensitive quantitation of vancomycin in plasma samples using liquid chromatography-tandem mass spectrometry and oral bioavailability in rats"Journal of Chromatography B. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Zhaopeng Hu, YV Pama Prasad, R Tawa, T Konishi, M Ishida et al.: "Diethyl ether fraction of Labrasol having a stronger absorption enhancing effect on gentamicin than Labrasol itself"International Journal of Pharmaceutics. 234. 223-235 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Zhaopeng Hu et al.: "A novel emulsifir, Labrasol, enhances gastrointestinal absorption of gentamicin"Life Science. 69. 2899-2910 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Zhaopeng Hu et al.: "Diethyl ether fraction of Labrasol having a stronger absorption enhancing effect on gentamicin than Labrasol itself"International Journal of Pharmaceutics. 234. 223-235 (2002)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi