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Endocrine Disrupting Effects of Airborne Particle Matter and Their Responsible Constituents

Research Project

Project/Area Number 13672342
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental pharmacy
Research InstitutionKanazawa University

Principal Investigator

KIZU Ryoichi  Kanazawa Univeristy Graduate School of Natural Science and Technology Associate Professor, 大学院・自然科学研究科, 助教授 (80143915)

Co-Investigator(Kenkyū-buntansha) HAYAKAWA Kazuichi  Kanazawa Univeristy Graduate School of Natural Science and Technology Professor, 大学院・自然科学研究科, 教授 (40115267)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordsairborne suspended participate matter / antiestrogenic effect / antiandrogenic effect / antagonist / aryl hydrocarbon receptor / polycyclic aromatic hydrocarbon / MCF-7 cell / PC3 cell / 多環芳番族炭化水素 / PC-3細胞 / α-ナフトフラボン / ディーゼル車排気粉じん / エストロゲン様作用
Research Abstract

There is an increasing environmental concern on exogenious chemicals that exhibit adverse effects on endocrine systems of human and wild life, so-called endocrine disrupting substances. Endocrine disrupting effects of airborne suspended particulate matter (SPM) has also attracted much attention. Therefore, wecollected SPM in Kanazawa, Ishikawa, and Sapporo, Hokkaido, at April, July, October and January. Then, we assayed their endocrine disrupting effects in cultured human-originated cell lines and investigated the responsible constituents. The results obtained are described below. 1. Everey SPM samples exhibited the antiestrogenic and antiandrogenic effects. 2. At both Kanazawa and Sapporo, SPM samples showed greater effect in winter than in summer. This was considered to be due to increased energy consumption in winter compared with that in summer. 3. The endocrine disrupting effects of SPM samples were redued when the cells were treated with SPM samples in combination with an aryl hydrocarbon receptor (AhR) agonist. This results indicated that the endocrine disrupting effects were due in part to constituents acting as an AhR agonists. 4. Since polycyclic aromatic hydrocarbons (PAHs) are typical AhR agonists in environment, several PAHs were determined for their concentration in SPM samples and assayed for their endocrine disrupting effects. The results revealed that the endocrine disrupting effects of SPM samples are based in part on PAHs. 5. Competitive hormone receptor binding assay and yeast two-hybrid assay revealed that SPM samples contains the constituents capable to bind to estrogen receptor or androgen receptor as antagonists.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Ryoichi Kizu: "A Role of Aryl Hydrocarbon Receptor in the Antiandrogenic Effects of Polycyclic Aromatic Hydrocarbons in LNCaP Human Prostate Carcinoma Cells"Archives of Toxicology. 77(in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ning Tang: "Improvement of an Authentic HPLC System for Nitropolycyclic Aromatic Hydrocarbons : Removal of an Interfering Peak and Increasing in the Number of Analytes"Analytical Science. 19. 249-253 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Akira Toriba: "Method for Determining Monohydroxybenzo[a]pyrene Isomers Using Column-switchihg High-Performance Liquid Chromatography"Analytical Biochemistry. 312. 14-22 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kazuichi Hayakawa: "Comparison of Polycyclic Aromatic Hydrocarbons and Nitropolycyclic Aromatic Hydrocarbons in Airborne Particulates Collected in Downtown and Suburban Kanazawa, Japan"Atmospheric Environment. 36. 5535-5541 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hideki Sasaki: "Simultaneous Determination of Monohydroxybenzo[a]pyrene Positional Isomers by Reversed Phase Liquid Chromatography Connected Online to Electrospray Ionization Mass Spectrometry"Biomedical Chromatography. 16. 432-436 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kazumasa Okamura: "Antiestrogenic Activity of Extracts of Diesel Exhaust Particulate Matter in MCF-7 Breast Carcinoma cells"Polycyclic Aromatic Compounds. 22. 747-759 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 木津 良一: "機器分析化学"丸善. 155 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ryoichi Kizu: "A Role of Aryl Hydrocarbon Receptor in the Antiandrogenic Effects of Polycyclic Aromatic Hydrocarbons in LNCaP Human Prostate Carcinoma Cells"Archives of Toxicology. 77,in press.. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ning Tang: "Improvement of an Authentic HPLC System for Nitropolycyclic Aromatic Hydrocarbons : Removal of an Interfering Peak and Increasing in the Number of Analytes"Analytical Science. 19. 249-253 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Akira Toriba: "Method for Determining Monohydroxybenzo[a]pyrene Isomers Using Column-switching High-Performance Liquid Chromatography"Analytical Biochemistry. 314. 14-22 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kazuichi Hayakawa: "Comparison of Polycyclic Aromatic Hydrocarbons and Nitropolycyclic Aromatic Hydrocarbons in Airborne Particulates Collected in Downtown and Suburban Kanazawa, Japan"Atmospheric Environment. 36. 5533-5541 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hideki Sasaki: "Simultaneous Determination of Monohydroxybenzo[a]pyrene Positional Isomers by Reversed Phase Liquid Chromatography Connected Online to Electrospray Ionization Mass Spectrometry"Biomedical Chromatography. 16. 432-436 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kazumasa Okamura: "Antiestrogenic Activity of Extracts of Diesel Exhaust Particulate Matter in MCF-7 Breast Carcinoma cells"Polycyclic Aromatic Compounds. 22. 747-759 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ryoichi Kizu: "A role of aryl hydrocarbon receptor in the antiandrogenic effects of polycyclic aromatic hydrocarbons in LNCaP human prostate carcinoma cells"Archives of Toxicology. 77(印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Akira Toriba: "Method for determining monohydroxybenzo[a]pyrene isomers using column-switching high-performance liquid chromatography"Analytical Biochemistry. 312・1. 14-22 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ning Tang: "Improvement of an authentic HPLC system for nitropolycyclic aromatic hydrocarbons : removal of, an interfering peak and increasing in the number of analytes"Analytical Science. 19・2. 249-253 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kazuichi Hayakawa: "Comparison of polycyclic aromatic hydrocarbons and nitropolycyclic aromatic hydrocarbons in airborne particulates collected in downtown and suburban Kanazawa, Japan"Atmospheric Environment. 36. 5535-5541 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hideki Sasaki: "Simultaneous determination of monohydroxybenzo[a]pyrene positional isomers by reversed phase liquid chromatography connected online to electrospray ionization mass spectrometry"Biomedical Chromatography. 16. 432-436 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hitoshi Kakimoto: "Comparison of atmospheric polycyclic aromatic hydrocarbons and nitropolycyclic aromatic hydrocarbons in an industrialized city (Kitakyushu) and two commercial cities (Sapporo and Tokyo)"Journal of Health Science. 48・4. 370-375 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kazumasa Okamura: "Antiestrogenic activity of extracts of diesel exhaust particulate matter in MCF-7 human breast carcinoma cells"Polycyclic Aromatic Compounds. 22(1)(印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Toshiharu Hirose: "Estrogenic/antiestrogenic activities of benzo[α]pyrene monohydroxy derivatives"Journal of Health Science. 47(6). 552-558 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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