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Development of laboratory testing methods through isolation of pituitary specific genes.

Research Project

Project/Area Number 13672422
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionOsaka University

Principal Investigator

TATSUMI Keita  Osaka University Graduate School of Medicine, Instructor, 医学系研究科, 講師 (00222109)

Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordspituitary / lymphocytic hypophysitis / autoantibodies / PGSF1a / PGSF1 / PGSF2 / GH / Body Map / 遺伝子
Research Abstract

To isolate novel transcripts expressed specifically in this gland, identified three novel pituitary-specific transcripts which encode putative proteins: pituitary gland specific factor 1a (PGSF1a), PGSF1b and PGSF2 through generating pituitary BodyMap database. To understand the pathogenesis of lymphocytic hypophysitis and to diagnose this disease efficiently, we developed laboratory testing methods. By developing radioligand assay using recombinant human 35S-labeled protein, presence of autoantibodies against three pituitary-specific proteins, GH, PGSF1a and PGSF2, and 5 enzymes expressed in the pituitary gland, prohormone convertase (PC) 1/3, PC2, carboxypeptidase E (CPE), alpha enolase, and PC2 regulatory protein, 7B2 were analyzed. Anti-human GH, anti-PGSF1a, and anti-PGSF2 antibodies (Ab) were detected in patients with lymphocytic hypophysitis and other hypopituitarism, but were not detected in patients with non-functioning pituitary macroadenoma. Patients positive for either anti-PC1/3 or anti-7B2 Ab were significantly frequent among patients with nonfunctioning pituitary macroadenoma than in other pituitary diseases and healthy controls. None of the patients was positive for anti-PC2 Ab or anti-CPE Ab. Anti-alpha-enolase Ab were not suitable for specific diagnosis of lymphocytic hypophysitis or pituitary non-functioning adenoma. Detection of antibodies against GH, PGSF1a, PGSF2, PC1/3 and 7B2 may be useful for the diagnosis of lymphocytic hypophysitis.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Tatsumi K: "Frequent appearance of autoantibodies against prohormone convertase 1/3 and neuroendocrine protein 7B2 in patients with non-functioning pituitary macroadenoma."Endocrine. 22,3. 335-340 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tanaka S: "Anti-alpha-enolase antibodies in pituitary disease."Endocr J. 50,6. 697-702 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tanaka S: "Novel autoantibodies to pituitary gland specific factor 1a in patients with rheumatoid arthritis."Rheumatology (Oxford). 42,2. 353-356 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tanaka S: "Autoantibodies against four kinds of neurotransmitter receptors in psychiatric disorders."J Neuroimmunol. 141,1-2. 155-164 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tanaka S: "Autoantibodies against muscarinic cholinergic receptor in chronic fatigue syndrome."Int J Mol Med. 12,2. 225-230 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Iwasaki M: "Validation of the Loop-Mediated Isothermal Amplification Method for Single Nucleotide Polymorphism Genotyping with Whole Blood."Genome Letters. 2,3. 119-126 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tatsumi K, Tanaka S, Takano T, Tahara S, Murakami Y, Takao T, Hashimoto K, Kato Y, Teramoto A, Amino N: "Frequent appearance of autoantibodies against prohormone convertase 1/3 and neuroendocrine protein 7B2 in patients with non-functioning pituitary macroadenoma."Endocrine. 22,3. 335-340 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tanaka S, Tatsumi K, Takano T, Murakami Y, Takao T, Yamakita N, Tahara S, Teramoto A, Hashimoto K, Kato Y, Amino N: "Anti-alpha-enolase antibodies in pituitary disease."Endocr J. 50,6. 697-702 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tanaka S, Tatsumi K, Tomita T, Kimura M, Takano T, Yoshikawa H, Amino N: "Novel autoantibodies to pituitary gland specific factor la in patients with rheumatoid arthritis."Rheumatology (Oxford). 42,2. 353-356 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Miyai K, Minekawa T, Tsutsumi S, Tatsumi K, Amino N: "Development of a radiotransporter assay for determination of iodide: preliminary studies."Clinica Chimica Acta. 339,1-2. 49-55 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Iwasaki M, Yonekawa T, Otsuka K, Suzuki W, Nagamine K, Hase T, Tatsumi K, Horigome T, Notomi T, Kanda H: "Validation of the Loop-Mediated Isothermal Amplification Method for Single Nucleotide Polymorphism Genotyping with Whole Blood."Genome Letters. 2,3. 119-126 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tanaka S, Matsunaga H, Kimura M, Tatsumi K, Hidaka Y, Takano T, Uema T, Takeda M, Amino N: "Autoantibodies against four kinds of neurotransmitter receptors in psychiatric disorders."J Neuroimmunol. 141,1-2. 155-164 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tanaka S, Kuratsune H, Hidaka Y, Hakariya Y, Tatsumi K, Takano T, Kanakura Y, Amino N: "Autoantibodies against muscarinic cholinergic receptor in chronic fatigue syndrome."Int J Mol Med. 12,2. 225-230 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tatsumi K: "Frequent appearance of autoantibodies against prohormone convertase 1/3 and neuroendocrine protein 7B2 in patients with non-functioning pituitary macroadenoma"Endocrine. 22,3. 335-340 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tanaka S: "Anti-alpha-enolase antibodies in pituitary disease"Endocr J. 50,6. 697-702 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tanaka S: "Novel autoantibodies to pituitary gland specific factor 1a in patients with rheumatoid arthritis"Rheumatology (Oxford). 42,2. 353-356 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tanaka S: "Autoantibodies against four kinds of neurotransmitter receptors in psychiatric disorders"J Neuroimmunol. 141,1-2. 155-164 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tanaka S: "Autoantibodies against muscarinic cholinergic receptor in chronic fatigue syndrome"Int J Mol Med. 12,2. 225-230 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Iwasaki M: "Validation of the Loop-Mediated Isothermal Amplification Method for Single Nucleotide Polymorphism Genotyping with Whole Blood"Genome Letters. 2,3. 119-126 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tanaka S: "Expression profile of active genes in the human pituitary gland"J Mol Endocrinol. 28,1. 33-44 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tanaka S: "Detection of autoantibodies against the pituitary-specific proteins in patients with lymphocytic hypophysitis"Eur J Endocrinol. 147,6. 767-775 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kimura M: "Anti-CYP2D6 antibodies detectied by quantitative radioligand assay and relation to antibodies to liver-specific arginase in patients with autoimmune hepatitis"Clin Chim Acta. 316,1-2. 155-164 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Izumi Y: "Development of liver dysfunction after delivery is possibly due to postpartum autoimmune hepatitis. A report of three cases"J Intern Med. 252,4. 361-367 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Izumi Y: "Simple and practical parameters for differentiation between destruction-induced thyrotoxicosis and Graves'thyrotoxicosis"Clin Endocrinol(Oxf). 57,1. 51-58 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 巽 圭太: "先天性下垂体ホルモン複合欠損症"最新医学. 57. 2657-2662 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tanaka, S.: "Expression profile of active genes in the human pituitary gland"J. Mol. Endocrinol.. 28・1. 33-34 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kimura, M.: "Anti-CYP2D6 antibodies detected by guantative radioligand assay and relation to antibodies to liver-specific arginase in patients with autoimmune hepatitis"Clin. Chim. Acta.. 316・1-2. 155-164 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Izumi, Y.: "Analysis of the KAL1 gene 19 Japanese patients with Kallmann syndrome"Endocr. J.. 48・2. 143-149 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Watanabe, K.: "Characteristics of experimental autoimmune hypophysitis in rats : major antigens are growth hormone, thyrotropin, and Latinizing hormone in this model"Autoimmunity. 33・4. 265-274 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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