Gene expressions of IGP-I, FGFs, and MyoD following denervation and compensatory overload.
| Project/Area Number |
13680044
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
体育学
|
|---|
| Research Institution | Health Sciences University of Hokkaido |
|---|
Principal Investigator |
YAMAGUCHI Akihiko Health Sciences University of Hokkaido, School of Dentistry, Assistant professor, 歯学部, 助教授 (50244869)
|
|---|
| Project Period (FY) |
2001 – 2002
|
| Project Status |
Completed (Fiscal Year 2002)
|
| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥1,000,000 (Direct Cost: ¥1,000,000)
|
|---|
| Keywords | compensatory overload / muscle hypertrophy / myosin heavy chain isoforms / IGF-I / FGF / MyoD |
|---|
| Research Abstract |
This study was undertaken to investigate the gene expressions of IGF-I, FGFs and MyoD following denervation and compensatory overload. 10 wk-olds Wistar strain rats were used in this study. The rats were divided into 4 groups, innervated and compensatory overloaded group (NM-OL), innervated and sham operated group (NM-Cont), denervated and compensatory overloaded group (DN-OL), and denervated and sham operated group (DN-Cont). IGF-I, PGFs and MyoD mRNA expressions as well as weights of plantaris muscle were measured 3, 7 and 21 days, and myosin heavy chain isoforms were measured 21 days after the treatments. At 21 days, type I myosin heavy chain isoform in NM-OL and DN-OL were significantly increased as compared with NM-Cont and DN-Cont, respectively. At 3, 7 and 21 days, muscle weights of the plantaris in NM-OL and DN-OL were significantly increased as compared with NM-Cont and DN-Cont, respectively. At 3, 7 and 21 days, FGF-2 mRNA and FGF-6 mRNA expressions in NM-OL and DN-OL were increased as compared with NM-Cont and DN-Cont, respectively. At 3 and 7 days, IGF-I mRNA and MyoD mRNA expressions in NM-OL and DN-OL were increased as compared with NM-Cont and DN-Cont, respectively. The plantaris muscles were strophied by the denervation. The expressions of FGF-2 mRNA and FGF-6 mRNA in DN-Cont and DN-OL were lower than the NM-Cont and NM-OL, respectively, however, the expressions of IGF mRNA and MyoD mRNA in DN-Cont and DN-OL were higher or unchanged. Thus it would seem to be likely that IGP-I, FGFs and MyoD were completely associated with the morphological changes of plantaris muscle following the denervation and compensatory overload.
|
Report
(3 results)
Research Products
(2 results)
