Gene expressions of IGP-I, FGFs, and MyoD following denervation and compensatory overload.

• Project/Area Number: 13680044
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 体育学
• Research Institution: Health Sciences University of Hokkaido
• Principal Investigator: YAMAGUCHI Akihiko Health Sciences University of Hokkaido, School of Dentistry, Assistant professor, 歯学部, 助教授 (50244869)
• Project Period (FY): 2001 – 2002
• Project Status: Completed (Fiscal Year 2002)
• Budget Amount: ¥1,700,000 (Direct Cost: ¥1,700,000); Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000); Fiscal Year 2001: ¥1,000,000 (Direct Cost: ¥1,000,000)
• Keywords: compensatory overload / muscle hypertrophy / myosin heavy chain isoforms / IGF-I / FGF / MyoD

Research Abstract

This study was undertaken to investigate the gene expressions of IGF-I, FGFs and MyoD following denervation and compensatory overload. 10 wk-olds Wistar strain rats were used in this study. The rats were divided into 4 groups, innervated and compensatory overloaded group (NM-OL), innervated and sham operated group (NM-Cont), denervated and compensatory overloaded group (DN-OL), and denervated and sham operated group (DN-Cont). IGF-I, PGFs and MyoD mRNA expressions as well as weights of plantaris muscle were measured 3, 7 and 21 days, and myosin heavy chain isoforms were measured 21 days after the treatments. At 21 days, type I myosin heavy chain isoform in NM-OL and DN-OL were significantly increased as compared with NM-Cont and DN-Cont, respectively. At 3, 7 and 21 days, muscle weights of the plantaris in NM-OL and DN-OL were significantly increased as compared with NM-Cont and DN-Cont, respectively. At 3, 7 and 21 days, FGF-2 mRNA and FGF-6 mRNA expressions in NM-OL and DN-OL were increased as compared with NM-Cont and DN-Cont, respectively. At 3 and 7 days, IGF-I mRNA and MyoD mRNA expressions in NM-OL and DN-OL were increased as compared with NM-Cont and DN-Cont, respectively. The plantaris muscles were strophied by the denervation. The expressions of FGF-2 mRNA and FGF-6 mRNA in DN-Cont and DN-OL were lower than the NM-Cont and NM-OL, respectively, however, the expressions of IGF mRNA and MyoD mRNA in DN-Cont and DN-OL were higher or unchanged. Thus it would seem to be likely that IGP-I, FGFs and MyoD were completely associated with the morphological changes of plantaris muscle following the denervation and compensatory overload.

Research Products

• [Publications] Akihiko Yamaguchi: "Local changes of IGF-I mRNA, GH receptor mRNA, and fiber size in rat plantaris muscle following compensatory overload"Japanese journal of Physiology. 53. 53-60 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Akihiko Yamaguchi, Yasuhiro Ikeda, Toshihiro Hirai, Takahiko Fujikawa, and Isao Morita: "Local changes of IGF-I mRNAm GH receptor mRNA, and fiber size in rat plantaris muscle following compensatory overload"Japanese journal of Physiology. 53. 53-60 (2003)
  • Description: 「研究成果報告書概要(欧文)」より