| Project/Area Number |
13680146
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
食生活
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| Research Institution | Aichi University of Education |
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Principal Investigator |
HAYASE Kazutoshi Aichi University of Education, Department of Home Economics, Professor, 教育学部, 教授 (10144180)
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| Co-Investigator(Kenkyū-buntansha) |
YOKOGOSHI Hidehiko The University of Shizuoka, School of Food and Nutritional Sciences, Professor, 食品栄養科学部, 教授 (70109320)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2001: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | estrogen / dietary protein / ovariectomy / isoflavone / protein synthesis / brain / rats |
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| Research Abstract |
The dietary protein and deficiency of sex hormones affect brain function, but relatively insufficient information is available on dietary and hormonal factors that moderate the molecular mechanism responsible for the changes.
The purpose of this study was to determine whether sex hormone, dietary isoflavone and dietary protein affects the brain protein synthesis in ovariectomized female rats and aged male rats. The fractional rates of protein synthesis in brain regions of ovariectomized rats treated with estrogen or genistein were significantly greater than that in ovariectomized rats. In ovariectomized female rats, the fractional rates of protein synthesis in the brain declined with a decrease of the quality of dietary protein, and increased with an addition of dietary methionine to low soy protein. In brain of all experiments, the RNA activity [g protein synthesized/(g RNA・d)] was significantly correlated with the fractional rate of protein synthesis. The RNA concentration (mg RNA/g p
… More
rotein) was not related to the fractional rate of protein synthesis in any organ. The aggregation in brain ribosomes declined with a decrease of the quantity and quality of dietary protein in aged rats.
The results strongly suggest that estrogen or isoflavone treatments of ovariectomized female rats are likely to increase the protein synthesis rate in the brain, and that the rate of protein synthesis in the brains depends on the quality of dietary protein and amino acids in ovariectomized female rats. The RNA activity is at least partly related to the fractional rate of brain protein synthesis. On the other hands, the higher quantity and quality of dietary protein improves the polysome profile in the brain of aged rats. Therefore, the changes in dietary protein may have controlled the translational phase of protein synthesis and been one of the factors affecting brain protein synthesis in aged rats. The present results also indicate the effects of estrogen, isoflavone and dietary protein on brain protein synthesis in ovariectomized female rats and aged rats are of importance in understanding the relationship among aging, nutrition, sex hormones and brain function in mammals. Less
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