Effects of combined intervention of soy isoflavone and moderate exercise on bone loss in ovariectomized mice

• Project/Area Number: 13680175
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 食生活
• Research Institution: National Institute of Health and Nutrition
• Principal Investigator: ISHIMI Yoshiko National Institute of Health and Nutrition, Lab Chief, 室長 (50154159)
• Co-Investigator(Kenkyū-buntansha): HIGUCHI Mitsuru Waseda University, Professor, 教授 (20192289)
• Project Period (FY): 2001 – 2003
• Project Status: Completed (Fiscal Year 2003)
• Budget Amount: ¥3,400,000 (Direct Cost: ¥3,400,000); Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000); Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000); Fiscal Year 2001: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: Osteoporosis / Soybean isoflavone / Soymilk / Exercise / Estrogen / 老化促進マウス / 大豆 / イソフラボン

Research Abstract

Body fat accumulation and bone loss are both often associated with estrogen deficiency following menopause. In this study, we examined whether soy isoflavone, one of the phytoestrogens, and moderate exercise interventions exhibit cooperative effects on body composition and bone mass in ovariectomized (OVX) mice. Eight week-old female mice were assigned to six groups : (1)sham-operated (sham) ; (2)OVX ; (3)OVX with received a soy isoflavone diet (OVX+ISO) ; (4)OVX with exercised on a treadmill (OVX+EX) ; (5)OVX with given both isoflavone and exercise (OVX+ISO&EX) ; and (6)OVX with treated with 17 β-estradiol subcutaneously (OVX+E2). Body composition and bone mineral density (BMD) were estimated by dual-energy X-ray absorptiometry (DXA). After the 6-week intervention, whole body fat (%) in the OVX group showed significantly higher than that in the sham group. Intervention of exercise and isoflavone alone partially inhibited OVX-induced body fat gain, and the combined intervention as well as E2 treatment completely restored fat mass to the sham level. Lean body mass in the whole body was not different in OVX group compared with that in OVX+ISO, OVX+EX and OVX+E2 groups, but it was significantly higher in OVX+ISO&EX than in other groups. BMD of the whole body, lumbar spine or femur showed significantly reduced by OVX, and the bone loss was partially inhibited by intervention of exercise or isoflavone alone. However, the combined intervention completely restored the bone mass to the level of sham, as did E2. Serum total cholesterol was significantly increased by OVX, and which was normalized by the combined intervention or E2 treatment. These results demonstrate that combined intervention of soybean isoflavone and exercise prevented body fat accumulation in the whole body with an increase in lean body mass and restoration of bone mass, and reduced high serum cholesterol in OVX mice.

Research Products

• [Journal Article] Combined intervention of soy isoflavone and moderate exercise prevents fat elevation and bone loss in ovariectomized mice. (2004)
  • Author(s): Wu J, Wang X, Chiba H, Higuchi M, Tnakatani N, Ezaki 0, Cui H, Yamada K, Ishimi Y
  • Journal Title: Metabolism 54 : 1536-154,2004 53.7 Pages: 942-948
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] 「研究成果報告書概要(欧文)」より (2004)
  • Author(s): Wu, J., Wang, X., Chiba, H., Higuchi, M., Tnakatani, N., Ezaki, O., Cui, H., Yamada, K., Ishimi, Y.
  • Journal Title: Metabolism 53 Pages: 942-948
• [Journal Article] Combined intervention of exercise and genistein prevented androgen deficiency-induced bone Loss in mice. (2003)
  • Author(s): Wu J, Wang XX, Chiba H, Higuchi M, Takasaki M, Ohta A, Yamada K, Ishimi Y.
  • Journal Title: J Appl Physiol 94.10 Pages: 335-342
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A combination of dietary fructooligosaccharides and isoflavone conjugates increases femoral bone mineral densiy and equal production in ovariectomized mice. (2003)
  • Author(s): Ohta A, Uehara M, Sakai K, Adlercreutz H, Morohashi T, Ishimi Y.
  • Journal Title: J Nutr 133.6 Pages: 1892-1897
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Puerariae Radix Prevents Bone Loss in Ovariectomized Mice (2003)
  • Author(s): Wang X, Wu J, Chiba H, Umegaki, Yamada K, Ishimi Y
  • Journal Title: J.Bone Miner.Metab. 21.5 Pages: 268-275
  • NAID: 50000288926
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] 「研究成果報告書概要(欧文)」より (2003)
  • Author(s): Wu, J., Wang, XX., Chiba, H., Higuchi, Takasaki, M., Ohta, A., Yamada, K., Ishimi, Y.
  • Journal Title: J Appl Physiol 94 Pages: 335-342
• [Journal Article] 「研究成果報告書概要(欧文)」より (2003)
  • Author(s): Ohta, A., Uehara, M., Sakai, K., Adlercreutz, H., Morohashi, T., Ishimi, Y.
  • Journal Title: J Nutr 133 Pages: 1892-1897
• [Journal Article] 「研究成果報告書概要(欧文)」より (2003)
  • Author(s): Wang, X., Wu, J., Chiba, H., Umegaki, K., Yamada, K., Ishimi, Y.
  • Journal Title: J.Bone Miner.Metab. 21 Pages: 268-275
• [Journal Article] Genistein, a soybean isoflavone, affects bone marrow lymphopoiesis and prevents bone loss in castrated male mice (2002)
  • Author(s): Ishimi Y, Yoshida M, Wakimoto S, Wu J, Chiba H, Wang XX, Takeda K, Miyaua C.
  • Journal Title: Bone 31.7 Pages: 180-185
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] 「研究成果報告書概要(欧文)」より (2002)
  • Author(s): Ishimi, Y., Yoshida, M., Wakimoto, S., Wu, J., Chiba, H., Wang, XX, Takeda, K., Miyaua, C.
  • Journal Title: Bone 31 Pages: 180-185
• [Journal Article] Cooperative effects of exercise and genistein administration on bone mass in ovariectomized mice. (2001)
  • Author(s): Wu J, Wang XX, Takasaki M, Ohta A, Higuchi M, Ishimi Y
  • Journal Title: J.Bone Miner.Res.16 : 1829-1836, 16.10 Pages: 1829-1836
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] 「研究成果報告書概要(欧文)」より (2001)
  • Author(s): Wu, J., Wang, XX., Takasaki, M., Ohta, A., Higuchi, M., Ishimi, Y.
  • Journal Title: J.Bone Miner.Res. 16 Pages: 1829-1836
• [Publications] Wu J, Wang X, Chiba H, Higuchi M, Yamada K, Ishimi Y: "Combined intervention of soy isoflavone and moderate exercise prevents body fat elevation and bone loss in OVX mice."Metabolism. in press. (2004)
• [Publications] Wu J, Wang X, Chiba H, Higuchi M, Takasaki M, Ohta A, Ishimi Y: "Combined intervention of exercise and genistein prevented androgen-deficiency-induced bone loss in mice."J.Appl.Physiol.. 94・10. 335-342 (2003)
• [Publications] Chiba H, Uehara M, Wu J, Wang X, Masuyama R, Suzuki K, Kanazawa K, Ishimi Y: "Hesperidin, a citrus flavonoid, inhibits bone loss and decreases serum and hepatic lipids in OVX mice."J.Nutr.. 133・6. 1892-1897 (2003)
• [Publications] Wang X, Wu J, Chiba H, Umegaki K, Yamada K, Ishimi Y: "Puerariae radix prevents bone loss in ovariectomized mice"J.Bone Miner.Metab.. 21・5. 268-275 (2003)
• [Publications] 石見佳子: "骨代謝と運動・ホルモン"体育の科学. 54・1. 37-42 (2004)
• [Publications] Ishimi Y, Yoshida M, Wakimoto S, Wu J, Chiba H, Way X, Takodu K, Miyamura C: "Genistein, a Soybean Isoflavone, Affects Bone Mallow Lymphopoiesis and Prevents Bone Loss in Castrated Male Mice"Bone. 31. 180-185 (2002)
• [Publications] Ohta A, Uehara M, Sakai K, Adlercreutz H, Morohashi, T, Ishimi, Y: "A combination of dietary fructoologosaccharides and isoflavone conjugates increases femoral bone mineral density and equol production in ovariectomized mice"J, Nutr. 132. 2048-2054 (2002)
• [Publications] Way XX, Wu J, Shidoji Y, Muto Y, Ohidhi N, Yagi K, Shinki T, Suda S, Ishimi Y: "Effects of geranylgeranoic acid in bone : induction of osteoblast differentiation and inhibition of osteoclast formation"J. Bone Miner. Res. 17. 91-100 (2002)
• [Publications] Wu J, Way XX, Chiba H, Higuchi M, Takasaki M, Ohta A, Ishimi Y: "Combined intervention of exercise and genistein prevented androgen deficiency-induced bone loss in mice"J. Appl Physiol.. 94. 335-342 (2003)
• [Publications] Wu J, Wany X, Takasaki M, Ohta A, Higuchi M, Ishimi Y: "Cooperative effects of exercise and genistein administration on bone mars in ovariectomized mice"J. Bone Miner. Res. 16(10). 1829-1836 (2001)
• [Publications] 石見佳子: "大豆イソフラボンと骨粗鬆症"治療. 83(6). 128-129 (2001)
• [Publications] Ishimi Y, Yoshida M, Wakimoto S, Wu J, Chiba H, Wang X, Takeda L, Hiyama C: "Genistein, a soybean isoflavone, effects bone marrow lymphopoiesis and prevents bone loss in castrated male mice"Bone. (in press). (2002)
• [Publications] Ohta A, Uehara M, Sakai K, Takasaki M, Adlecreutx H, Morohashi T, Ishimi Y: "Combination of dietory fluctooligosaccharides and isoflavone conjugates increases femoral bone mineral density and equal production in ovariectomixed mice"J Nutr. (in press). (2002)
• [Publications] 石見佳子(分担): "食品大百科辞典"食品総合研究所編、朝倉書店. 527-531 (2001)